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Macromolecular Crystallography

Beamline P14.EH2

Annual Call for Proposals 2025
The call for proposals to apply for beamtime between March and December 2025 is now CLOSED. Rolling proposal submission via the EMBL user portal will be available in spring 2025 again.

Time-resolved Crystallography ‘T-REXX’

A second endstation dedicated to pump-probe time-resolved macromolecular crystallography, T-REXX (Time-resolved experiments with crystallography), is in operation on the EMBL beamline P14 on PETRA III since late 2018. The development and the construction of the endstation was realized in a collaborative project between Hamburg University (Arwen Pearson) and EMBL Hamburg (Thomas R. Schneider) funded by a collaborative ‘Verbundforschung’ grant from the German Ministry for Research and Education, BMBF.

The endstation features a fixed energy (12.7 keV) beam with a flux of ~2 x 1012 ph/s through a 15 x 10 µm2 cross section. While the endstation is not equipped with a standard diffractometer, it offers various means for beam conditioning, and pre-installed lasers and laser-optics.  The sample space is open and accessible so that different sample presentation and/or pump-probe technologies can be installed.

As the T-REXX endstation is physically separated from the first endstation of P14, elaborate and time-consuming setup works for pump-probe time-resolved experiments can be performed without impeding the standard user operation on beamline P14. Only once the instrumentation for a time-resolved experiment is in place, the beam is then guided to the T-REXX endstation.

T-REXX Endstation with a crystallographic chip
T-REXX endstation with a crystallographic chip featuring ca. 20000 well for storing protein micro-crystals mounted on an xy-table. Foto: EMBL/Kinga Lubowiecka

External links

An explanation of time-resolved serial crystallography.

Contact

Beamline Scientist
David von Stetten
Tel: +49 (0) 40 89902-235
Email: Send a mail

MX@PETRA3 coordinator
Thomas R. Schneider
Tel.:+49 (0) 40 89902-111

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