In situ CLEM at room temperature and in cryo

Time (Europe/Berlin)	Speaker	Location
14:00 – 15:00	Welcome, house notes and course overview	ISG Hotel
15:00 – 16:00	Lecture 1: How to choose your CLEM approach Ori Avinoam – Weizmann Institute of Science, Israel	ISG Hotel
16:00 – 16:30	Coffee Break	ISG Hotel
16:30 – 18:00	Flash Talks	ISG Hotel
18:00 – 19:00	Lecture 2: In situ CLEM Workflow Rachel Mellwig – EMBL Heidelberg, Germany	ISG Hotel
19:00	Dinner	ISG Hotel

Time (Europe/Berlin)	Speaker	Location
08:40 – 09:00	Transfer to EMBL from ISG Hotel	ISG Hotel
09:00 – 09:30	Check in, safety briefing and presentation on responsible conduct in research (RCR)	Flex Lab A & B
09:30 – 10:30	Lecture 3: Correlative multi-modal imaging in three dimensions Yannick Schwab – EMBL Heidelberg, Germany	Flex Lab A & B
10:30 – 10:45	Coffee break + Meet the Speakers	Flex Lab A & B
10:45 – 11:45	Lecture 4: Tips and tricks to adapt on-section CLEM to diverse biological questions Mandy Boermel – EMBL Heidelberg, Germany	Flex Lab A & B
11:45 – 12:45	Lecture 5: Strategies for cryoEM sample preparation Evgenia Zagoriy – EMBL Heidelberg, Germany	Flex Lab A & B
12:45 – 13:30	Lunch	EMBL Canteen
13:30 – 18:30	Practical 1A: High pressure freezing Anna Steyer – EMBL Heidelberg, Germany Andreia Pinto – Leica Microsystems, Germany Practical 1B: Freeze substitution Paolo Ronchi – EMBL Heidelberg, Germany Ori Avinoam – The Weizmann Institute of Science, Israel Practical 1C: Plunge freezing Zhengyi Yang – EMBL Heidelberg, Germany Léa Swistak – EMBL Heidelberg, Germany Practical 1D: Micropatterning Ievgeniia Zagoriy – EMBL Heidelberg, Germany Herman Fung – University of Michigan, USA
18:30 – 19:30	Dinner	EMBL Canteen
19:30 – 19:45	Transfer to ISG Hotel from EMBL	ATC Entrance

Time (Europe/Berlin)	Speaker	Location
08:40 – 09:00	Transfer to EMBL from ISG Hotel	ISG Hotel
09:00 – 10:00	Lecture 6: Tricks and pitfalls in ultramicrotomy Viola Oorschot – EMBL Heidelberg, Germany Andreia Pinto – Leica Mycrosystems, Germany	Flex Lab A & B
10:00 – 11:00	Lecture 7: Cryo-CLEM to study viruses Petr Chlanda – University of Heidelberg, Germany	Flex Lab A & B
11:00 – 11:15	Coffee break + Meet the Speakers	Flex Lab A & B
11:15 – 12:15	Lecture 8: In block fluorescence imaging for FIB-SEM targeting Paolo Ronchi – EMBL Heidelberg, Germany	Flex Lab A & B
12:15 – 13:00	Lunch	EMBL Canteen
13:00 – 19:00	Practical 2A: Ultramicrotomy at room temperature Andreia Pinto – Leica Microsystems, Germany Viola Oorschot – EMBL Heidelberg, Germany Practical 2B: Light microscopy on plastic sections Paolo Ronchi – EMBL Heidelberg, Germany Practical 2C: Cryo light microscopy (LSM900 Zeiss) Anna Steyer – EMBL Heidelberg, Germany Zhengyi Yang – EMBL Heidelberg, Germany Practical 2D: Cryo light microscopy (Leica Stellaris) Martin Fritsch – Leica Microsystems, Germany Ievgeniia Zagoriy – EMBL Heidelberg, Germany
19:00 – 19:20	Transfer to ISG Hotel from EMBL	ATC Entrance
19:20 – 20:20	Dinner	ISG Hotel

Time (Europe/Berlin)	Speaker	Location
08:40 – 09:00	Transfer to EMBL from ISG Hotel	ISG Hotel
09:00 – 10:45	Lecture 9: Quantifying UA within your sample using EDS Pedro Machado – Oxford Instruments, UK	Flex Lab A & B
09:45 – 10:45	Practical 3A: SerialEM demonstration with Remote TEM session Ori Avinoam – The Weizmann Institute of Science, Israel Mandy Boermel – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany	Computer Training Lab
10:45 – 11:00	Coffee break + Meet the Speakers	Computer Training Lab
11:00 – 12:00	Practical 3B: SerialEM hands-on Mandy Boermel – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Ori Avinoam – The Weizmann Institute of Science, Israel Giulia Mizzon – Universitaetsklinikum Heidelberg, Germany	Computer Training Lab
12:00 – 13:00	Lunch	EMBL Canteen
13:00 – 15:00	Practical 3C: Icy and ec-CLEM Perrine Paul-Gilloteaux – University of Nantes, France Nadav Scher – EMBL Heidelberg, Germany	Computer Training Lab
15:00 – 15:15	Coffee break + Meet the Speakers	Computer Training Lab
15:15 – 15:45	Debrief: Wrap up of first round of practicals that have introduced the entire workflow, Q&A	Computer Training Lab
15:45 – 16:00	Group photo
16:00 – 16:45	Transfer to Downtown (with a stop at ISG Hotel)	ATC Entrance
16:45 – 19:00	Free time in Heidelberg city centre
19:00 – 22:00	Course dinner	Vetter´s Alt Heidelberger Brauhaus

Time (Europe/Berlin)	Speaker	Location
08:40 – 09:00	Transfer to EMBL from ISG Hotel	ISG Hotel
09:00 – 10:00	Lecture 10: Lessons Learned from 3D-CLEM Nadav Scher – Weizmann Institute of Science, Israel	Flex Lab A & B
10:00 – 10:15	Coffee break + Meet the Speakers	Flex Lab A & B
10:15 – 11:00	Lecture 11: Room temperature CLEM Success Story Mathilda Lennartz – Max Planck Institute of Molecular Cell Biology and Genetics, Germany	Flex Lab A & B
11:00 – 11:45	Lecture 12: Cryo-CLEM Success Story Léa Swistak – EMBL Heidelberg, Germany	Flex Lab A & B
11:45 – 12:20	Lunch	EMBL Canteen
12:20 – 13:00	EMBL Imaging Centre and TREC truck
13:00 – 19:00	Practical 4A: HPF and FS – more in depth Rachel Mellwig – EMBL Heidelberg, Germany Ori Avinoam – The Weizmann Institute of Science, Israel Viola Oorschot – EMBL Heidelberg, Germany Practical 4B: In-depth software, hands-on session (ICY, SerialEM, MoBIE) Perrine Paul-Gilloteaux – University of Nantes, France Herman Fung – University of Michigan, USA Nadav Scher – EMBL Heidelberg, Germany Practical 4C: Ultramicrotomy including serial sectioning Viola Oorschot – EMBL Heidelberg, Germany Mandy Boermel – EMBL Heidelberg, Germany Practical 4D: Cryo light microscopy hands-on session Anna Steyer – EMBL Heidelberg, Germany Martin Fritsch – Leica Microsystems, Germany Steffen Klein – EMBL Heidelberg, Germany Zhengyi Yang – EMBL Heidelberg, Germany Practical 4E: Relate software demo Pedro Machado – Oxford Instruments , UK Practical 4F: Demo of the cryo-super resolution microscope Soheil Mojiri – EMBL Heidelberg, Germany Practical 4G: In block fluorescence imaging Paolo Ronchi – EMBL Heidelberg, Germany
19:00 – 20:00	Dinner	EMBL Canteen
20:00 – 20:15	Transfer to ISG Hotel from EMBL	ATC Entrance

Time (Europe/Berlin)	Speaker	Location
08:40 – 09:00	Transfer to EMBL from ISG Hotel	ISG Hotel
09:00 – 10:30	Round table discussions on open questions, students explain implementations for their own projects	Flex Lab A & B
10:30 – 10:45	Coffee break + Meet the Speakers	Flex Lab A & B
10:45 – 11:45	Round table discussions on open questions, students explain implementations for their own projects	Flex Lab A & B
11:45 – 12:15	Lecture 13: Outlook: 3D CLEM in cryo Steffen Klein – EMBL Heidelberg, Germany	Flex Lab A & B
12:15 – 12:30	Coffee break + Meet the Speakers	Flex Lab A & B
12:30 – 13:00	Lecture 14: Outlook: Probes for cryo CLEM Herman Fung – University Michigan, USA	Flex Lab A & B
13:00 – 13:30	Lecture 15: Outlook: Super Resolution cryo CLEM Soheil Mojiri – EMBL Heidelber, Germany	Flex Lab A & B
13:30 – 13:45	Feedback and Farewell	Flex Lab A & B
13:45 – 14:00	Packed Lunch
14:00	Transfer from EMBL to Heidelberg train station and city center	ATC Entrance

The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, meals and coffee breaks.

This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue

Academia	€500
PhD Student	€500
Industry	€1100

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waivers

The fee waiver will cover the registration sum that you have paid to attend the course.

Childcare grants

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

If you are attending virtually, you can apply for financial assistance in the submission portal by the abstract deadline. Read the instructions on how to apply for financial assistance. Only submissions for financial assistance will be accepted. Presentation abstracts cannot be submitted here and will be declined. 

In your application you will be asked to summarise your current work,  answer questions regarding why your lab cannot fund your attendance, and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

The scientific organisers will select the recipients of registration fee waivers during the participant selection process for courses. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Further details

Check out this list of external funding opportunities.

For further information about financial assistance please refer to the FAQ page.

Hotel

Accommodation in twin rooms, including breakfast, is included in the registration fee. Accommodation has been booked for you at the ISG Hotel for the default dates of 8 to 14 February. In case you would like to have a single room, please let the hotel know and please note that you will be required to pay an additional cost of 32€ per night (availability of single rooms may be limited). This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you.

We recommend that all course participants stay at the dedicated hotel for the course.

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk..

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

• Map to walk from ISG Hotel to EMBL

Technical requirements

Basic familiarity with light and electron microscopy techniques

Additional software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course.

Other information

While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.

Guidelines

• Do not broadcast the course to unregistered participants.
• Do not share your login details with others.
• You are encouraged to tweet and post about the event. Tweet unless the speaker specifically says otherwise, but be mindful of unpublished data. 
• Please do not capture, transmit or redistribute data presented at the meeting.

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in eCampus for you to enjoy during the event.

How to ask questions

Questions during and after the talks can be asked in the live streaming platform. If time runs out or you think of a question later, you can use the EMBL eCampus forum or send a direct message to the speaker/trainer.

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.

Please find additional information including FAQs and terms and conditions on our Information for Participants page.