Genome engineering: CRISPR/Cas in cells and mice

Overview

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Course overview

This course will provide training in genome editing and cell engineering in mammalian cells and mouse embryos using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knock-outs/knock-ins, and target validation using the most current technologies. The course will be split into two groups (mammalian cells OR mice) based on participants’ expertise.

Please note that all participants are expected to engage in all sessions. Participants should plan for approximately 1 hour extra per day for self paced learning modules.

Audience

This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and who want to learn how to create an engineered mammalian cell line or mouse model using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.

Modules/resources

• How to create a CRISPR/Cas9 experiment using various design tools
• Transfection optimisation for efficient gene editing
• Generation of knock-out and knock-in cell lines and mouse embryos
• Validation of genome-edited cell lines and embryos
  

Learning outcomes

After this course you should be able to:

• Design a CRISPR based project using editing tools for your target gene of interest
• Choose the right format of gene editing tool and delivery strategy for your cell type or embryos
• Edit, screen and validate the engineered models, embryo manipulation
  

What past participants say about the course

“The EMBL course about CRISPR applications for genome engineering was an incredible experience. From the quality of the lectures, the availability of the invited speakers to respond to all questions and doubts, the openness and diversity of participants to the great organization and the impressive facilities and core facility teams working there. It all created a perfect atmosphere for learning about this groundbreaking technique and the inspiring work being done with it as well as great networking. I highly recommend this course.” – Sofia Morazzo, International Clinical Research Center, Czech Republic

“The course covers all you need to organise your CRISPR experiments in great detail. The speakers are fantastic and eager to help with your specific questions. The organisation is also fantastic, where you only have to think on CRISPR and nothing else.” – Sergio G. San Miguel, Max Planck Institute for Infection Biology, Germany

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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