Jörg Bantin
BioRad,
Germany
Overview
Course Overview
Liquid biopsies have the advantage of being largely non-invasive and providing a vast amount of information for the detection and monitoring of diseases such as cancer. This course will provide an overview in the several techniques to detect and analyse circulating tumour cells (CTCs), cell free tumor DNA (ctDNA), miRNA in liquid biopsies, cell free tumor RNA (ctRNA) and proteins. Participants will be introduced to all aspects ranging from proper sampling through error free sequencing to data analysis. Additional lectures will cover extracellular vesicles and clinical aspects using liquid biopsies, especially in the field of cancer diagnostics.
Please note that all participants are expected to engage in all sessions.
Audience
This course targets young scientists entering the field of liquid biopsy analysis and wanting to learn about the benefits and limitations of different approaches.
Modules/Resources
- Ultra-sensitive mutation detection using sequencing
- Single cell analysis in liquid biopsies
- Digital PCR applications in the liquid biopsy workflow
- Protein analysis in liquid biopsies
- miRNA analysis in liquid biopsies
- Hands-on data analysis and interpretations
Learning Outcomes
Participants will receive an overview of various workflows in liquid biopsies, from sample preparation to data analysis. The course provides and overview of the current methods. Whereby a strong focus will be on experimental design, best practices, pitfalls as well as dos and don’ts. After the course, participants should be able to assess the best methods for their application as well as design their experiments.
What past participants say about the course:
“The Liquid Biopsy course gave me plenty of new insights for different applications of the technique in cancer. I learned new techniques, discussed my project with top peers and met a lot of highly motivated young professionals. I incorporated the learned applications in my research and this really improved my current project.” – Aleksei Tikhonov, Engelhardt Institute of Molecular Biology, RAS, Russian Federation
Speakers, Trainers and Organisers
Speakers and Trainers
Lei Chen
Uppsala University,
Sweden
Leonie de Visser
Roche Diagnostics International,
Switzerland
Anders Edsjö
Skåne University Hospital,
Sweden
Stefan Filges
Sahlgrenska Academy at University of Gothenburg,
Sweden
Amin Forootan
MultiD Analyses AB
Switzerland
Tony E. Godfrey
Boston University School of Medicine,
USA
An Hendrix
Ghent University
Belgium
Romy Kirsten
National Center for Tumor Diseases (NCT) Heidelberg,
Germany
Mikael Kubista
Institute of Biotechnology and TATAA Biocenter,
Czech Republic
Ulf Landegren
Uppsala University,
Sweden
Manuel Marceliano Luna Santa-María
Sahlgrenska Academy at University of Gothenburg,
Switzerland
Michael Pfaffl
Technical University of Munich,
Germany
Beata Ramasz
EMBL Heidelberg,
Germany
Anders Ståhlberg
Sahlgrenska Academy at University of Gothenburg,
Sweden
Valérie Taly
Paris Descartes University,
France
Thorsten Voss
QIAGEN,
Germany
Scientific Organisers
Vladimir Benes
EMBL Heidelberg,
Germany
Anders Ståhlberg
Sahlgrenska Academy at University of Gothenburg,
Sweden
Course Organiser
Lisa Trinh
EMBL Heidelberg
Germany
Programme
Date: 11 - 18 Sep 2020
Location: Virtual