NicE-RNA sequencing for multiomics on open chromatin and transcription

Overview

Course overview

This course will focus on the latest state-of-the-art novel library preparation protocols. We will start with experimental design, focusing on hands-on library preparation (including best practices, standards, and potential pitfalls), concluding with sequencing of libraries, bioinformatic evaluation & best practices.

Multiomic data in scientific projects have become more and more important. NicE-Seq is a novel method enabling detection of open chromatin in fixed low numbers of cells without any additional instrumentation. Combining open chromatin with transcriptome information is crucial for interpretation of cellular states. Therefore, the course participants will also prepare mRNA libraries from the same cell populations. 

Participants will carry out a complete workflow, from isolation of DNA & RNA from fixed cells, library preparation for both applications and shallow sequencing. The course will discuss alternative novel approaches, various cell fixatives and their impact on analysis. In addition, we will cover basic bioinformatic tools required for data processing, quality control and initial data analysis.

Beside the practical exercises, lectures and discussion sessions, the course will offer the opportunity to learn more about transferring the knowledge gained during the course to the participants’ home institutes.

Audience

This course is directed towards wet-lab biologists, scientists and technicians who want to apply the here offered technology.

Modules/resources

• Hands-on training on NGS multiome library preparations with upcoming state-of-the-art reagents
• Setting up a sequencing run
• Basics in data handling and analysis

Learning outcomes

Multiome genomic analyses have become important to understand genome dynamics. Methodology to explore this is still challenging and requires a lot of experience and hands on time.

During the course, its participants will:

• learn to apply novel NEBNext NGS library preparation reagents and protocols
• learn about the benefits and bottlenecks of different library preparations
• be exposed to cutting-edge sequencing technology

What past participants say about this course

“I really enjoyed this course (both practical and invited lectures). This was an interesting course format and I will apply the techniques I learned during the course in my project. This is an extremely valuable course and I recommend this course to my colleagues. Thanks to the EMBL organizers for making such a wonderful event successful.” – Kesava Priyan Parmasamy, Ulmea University, Sweden

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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Media kit

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