EMBL Course
This course will focus on the latest state-of-the-art novel library preparation protocols. We will start with experimental design, focusing on hands-on library preparation (including best practices, standards, and potential pitfalls), concluding with sequencing of libraries, bioinformatic evaluation & best practices.
Multiomic data in scientific projects have become more and more important. NicE-Seq is a novel method enabling detection of open chromatin in fixed low numbers of cells without any additional instrumentation. Combining open chromatin with transcriptome information is crucial for interpretation of cellular states. Therefore, the course participants will also prepare mRNA libraries from the same cell populations.
Participants will carry out a complete workflow, from isolation of DNA & RNA from fixed cells, library preparation for both applications and shallow sequencing. The course will discuss alternative novel approaches, various cell fixatives and their impact on analysis. In addition, we will cover basic bioinformatic tools required for data processing, quality control and initial data analysis.
Beside the practical exercises, lectures and discussion sessions, the course will offer the opportunity to learn more about transferring the knowledge gained during the course to the participants’ home institutes.
This course is directed towards wet-lab molecular biologists, scientists and technicians who want to learn best practice in NGS library preparations from various sample types.
Multiome genomic analyses have become important to understand genome dynamics. Methodology to explore this is still challenging and requires a lot of experience and hands on time.
During the course, its participants will:
“I really enjoyed this course (both practical and invited lectures). This was an interesting course format and I will apply the techniques I learned during the course in my project. This is an extremely valuable course and I recommend this course to my colleagues. Thanks to the EMBL organizers for making such a wonderful event successful.” – Kesava Priyan Parmasamy, Ulmea University, Sweden
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
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Date: 29 Sep - 2 Oct 2025
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Organisers:
Contact: Karoline Wolkersdorfer