Jochen Barths
ProSort
France
EMBL Course
Flow cytometry is a highly effective method for analysing individual cells that has become widely used in the fields of life sciences and medicine. By utilising flow cytometry, researchers can accurately identify and measure specific cell populations within complex mixtures, as well as assess cell cycle progression, proliferation status, and expression of fluorescent markers.
Our course aims to provide a comprehensive understanding of the underlying principles of flow cytometry, while also emphasising practical considerations related to the most frequently used assays. Through a combination of theoretical instruction and hands-on training, students will gain the knowledge and skills necessary to effectively design, execute, and interpret flow cytometry experiments.
The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of flow cytometry, data acquisition or analysis software (DIVA + FlowJo) is of advantage, but not strictly a must.
Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing! We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.
“My experience at EMBL was lovely and stimulating. The knowledge I acquired through this short time has improved my learning and practical skills. EMBL is a great place for scientific resources; a friendly environment both socially and professionally.” – Ibrahim Malami, Usmanu Danfodiyo University Sokoto
“The course was excellently organized, with high quality theoretical and practical sessions! It is a must for everyone interested in basic and more specific aspects of flow cytometry techniques.” – Filipe Olim, University of Madeira
“I enjoyed the course a lot and learned what I was looking for: an opportunity to learn the principles of flow cytometry that are important for planning and performing experiments. Besides the theoretical part, I was able to run samples at the instruments by myself which was a great starting point to perform experiments by myself in the future.” – Maya Funk, German Cancer Research Center
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Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.
ProSort
France
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Copenhagen
Denmark
German Cancer Research Center
Germany
EMBL Heidelberg
Germany
FlowJo Informatics, BD
Germany
EMBL Rome
Italy
EMBL Heidelberg
Germany
German Cancer Research Center
Germany
Sony Biotechnology Inc.
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
German Cancer Research Center
Germany
Training Lab Technician
EMBL Heidelberg
Germany
Course and Conference Manager
EMBL Heidelberg
Germany
Are you on social media? Post using #EMBLCytometry and don’t forget to tag @EMBLEvents
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:00 – 09:30 | Welcome, Introduction and Safety briefing | ATC Flex Lab |
09:30 – 10:45 | Participants flash talks | ATC Flex Lab |
10:45 – 11:15 | Coffee Break | ATC Flex Lab |
11:15 – 12:00 | Lecture: Fluidics and optics Gelo dela Cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | ATC Flex Lab |
12:00 – 12:45 | Lecture: Fluorescence and PMTs detectors Steffen Schmitt – German Cancer Research Center, Germany | ATC Flex Lab |
12:45 – 13:45 | Lunch | EMBL Canteen |
13:45 – 14:30 | Lecture: Data generation and data display Gelo dela Cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | ATC Flex Lab |
14:45 – 15:45 | Lecture: The Essentials of gating, FMO and gating controls Beata Ramasz – EMBL Heidelberg, Germany | ATC Flex Lab |
15:45 – 16:15 | Coffee Break | Flex Lab A & B |
16:15 – 18:00 | Practical Session 1: Assessing instrument performance | Training Lab B |
18:00 – 19:00 | Dinner | EMBL Canteen |
19:00 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | |
09:00 – 10:00 | Lecture: Fluorescence spillover and compensation Gerald Pfister – EMBL Heidelberg Rome | ATC Flex Lab |
10:00 – 10:30 | Coffee Break | ATC Flex Lab |
10:30 – 12:00 | Practical Session 2: Absolute counting | Training Lab B |
12:00 – 13:00 | Lunch | EMBL Canteen |
13:00 – 14:00 | Lecture: Experimental design for multi-color flow Diana Ordonez – EMBL Heidelberg, Germany | |
14:00 – 16:00 | Practical 3: Multi- color staining (Group 1) Practical 4: Data analysis with FlowJo (Group 2) | Group 1: Training Lab B Group 2: Computer Training Lab |
16:00 – 16:30 | Coffee Break | ATC Flex Lab |
16:30 – 18:30 | Practical 4: Data analysis with FlowJo (Group 1) Practical 3: Multi- color staining (Group 2) | Group 1: Computer Training Lab Group 2: Training Lab B |
18:30 | Departure to hotel | |
19:00 – 21:30 | Offsite dinner |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | |
09:00 – 09:30 | Q&A session | ATC Flex Lab |
09:30 – 10:30 | Lecture: Spectral Flow Cytometry Neda Tafrishi – Sony Biotechnology Inc., Germany | ATC Flex Lab |
10:30 – 11:00 | Coffee break | ATC Flex Lab |
11:00 – 12:00 | Lecture: Cell Cycle Analysis Diana Ordonez – EMBL Heidelberg, Germany | ATC Flex Lab |
12:00 – 13:00 | Lunch | EMBL Canteen |
13:00 – 15:00 | Practical 5: Cell cycle samples (Group 1) Practical 6: Data analysis with FlowJo session 2 (Group 2) | Group 1: Training Lab B Group 2: Computer Training Lab |
15:00 – 15:30 | Coffee break | ATC Flex Lab |
15:30 – 17:30 | Practical 6: Data analysis with FlowJo session 2 (Group 1) Practical 5: Cell cycle samples (Group 2) | Group 1: Computer Training Lab Group 2: Training Lab B |
17:30 – 18:30 | Dinner | EMBL Canteen |
18:30 | Departure to hotel and free evening |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | |
09:00 – 10:00 | Lecture: Publishing your flow cytometry data Marcus Eich – German Cancer Research Center, Germany | ATC Flex Lab |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 12:00 | Practical 7: Preparing Flow Data for Publication – FlowJo | Computer Training Lab |
12:00 – 13:00 | Lunch | |
13:00 – 14:00 | Lecture: Assessing cell death Steffen Schmitt – German Cancer Research Center, Germany | ATC Flex Lab |
14:00 – 15:30 | Practical 8: Measuring apoptosis | Training Lab B |
15:30 – 16:00 | Coffee Break | Flex Lab A & B |
16:00 – 17:30 | Practical 9: Data acquisition/Data Analysis | Training Lab B / Computer Training Lab |
17:45 | Departure to restaurant | ATC Entrance |
18:00 – 22:00 | Social Activity and Dinner |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | |
09:00 – 10:00 | Lecture: Basics of cell sorting Jochen Barths – ProSort, France | ATC Flex Lab |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 12:30 | Practical 10: Cell sorting and reanalysis of sorted cells | Training Lab A & B |
12:30 – 13:30 | Lunch | |
13:30 – 14:30 | Lecture: High-dimensional data analysis with FlowJo Ioannis Panetas – BD, Germany | ATC Flex Lab |
14:30 – 15:00 | Feedback and Goodbye | ATC Flex Lab |
15:00 | Departure to Heidelberg Train Station | ATC Entrance |
The course is limited to 16 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €600 |
PhD Student | €600 |
Industry | €1100 |
A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.
Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
– up to €1000 for participants travelling to an EMBL Course from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.
– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.
*Registration fees are only covered for EMBO Practical Courses
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
For EMBO Practical Course participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early-stage researchers. A maximum amount of €500 can be awarded per participant applying for an EMBO childcare grant.
Applies to selected courses only. Availability will be indicated during the motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 5 nights (Sunday 12 November to Friday 17 November 2023) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code CYT23-01). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
(delete the technical requirements part if it is not a computer based course in the courtyard room, as well as change label of the details section to only Event Software)
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Event Partners
Event Sponsors
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
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Date: 13 - 17 Nov 2023
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Deadline(s):
Closed
Organisers:
Contact: Nathalie Sneider