Heiko Gaethje
Evident Scientific
Germany
EMBL Course
Light microscopy is the most widely used form of microscopy for life science research. The correct handling of the microscope and optimising image quality (e.g. contrast, resolution) as well as experimental design according to different applications are crucial to obtain the best results.
This course will provide the participants with an overview of the basics of microscopy (light and optics, contrast techniques and imaging). The confocal part will include multicolour, 3D and time-lapse imaging as well as spectral imaging, photobleaching (FRAP, FLIP, FLAP) and photoactivation techniques. Basic image analysis tools including measurement functions, colocalisation and spectral unmixing as well as 3D/4D image processing will be covered, both in theory and practice. The course will combine lectures with intense daily hands-on practices followed by discussions.
This course is directed towards PhD students and faculty searching for instruction on light microscopy and basic image processing.
Participants will get an overview about the basics of microscopy.
.
Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.
.
This course is organised in cooperation with Evident-Olympus.
Evident Scientific
Germany
Evident Scientific
Germany
EMBL Heidelberg
Germany
Evident Scientific
Germany
Evident Scientific
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
Training Lab Technician
EMBL Heidelberg
Germany
Predoctoral Fellow
EMBL Heidelberg
Germany
Are you on social media? Post using #EMBLWidefieldConfocal and don’t forget to tag @EMBLEvents
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:00 – 09:15 | Arrival and welcome, house notes | Flex Lab A & B |
09:15 – 13:00 | Basics of optics and microscopy • Theoretical basics of light and optics • Types of microscope frames – and types of optics • Set up bright field microscopes and the appropriate optics for different needs • Cleaning, centering adjusting and operating a microscope • Basic set-up and use of imaging devices Hands-on session 1 | Flex Lab A & B |
13:00 – 14:00 | Lunch | EMBL Canteen |
14:00 – 15:00 | Contrast methods • Introduction into contrast methods (phase contrast and differential interference contrast/DIC) | Flex Lab A & B |
15:00 – 17:30 | Contrast methods – set up and alignment • Set up and use of contrast methods: phase contrast and differential interference contrast/DIC Hands-on session 2 | Flex Lab A & B |
17:30 – 18:00 | Discussing open questions | Flex Lab A & B |
18:00 – 19:00 | Dinner | EMBL Canteen |
19:00 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
08:50 – 09:00 | Arrival and coffee | Flex Lab A & B |
09:00 – 12:30 | Basics of digital imaging • Adaptation of digital cameras and their alignment • Interpretation of image content, histogram and digital filter functions • Improving digital image contrast | Flex Lab A & B |
12:30 – 13:30 | Lunch | EMBL Canteen |
13:30 – 16:00 | Fluorescence microscopy I • Introduction to fluorescence microscopy • Setting up of microscopes and cameras for fluorescence observation • The different types of illumination sources (e.g. mercury, xenon, metal halide or LED) • Cubes, filters and objectives • Find the appropriate filter combination for a given fluorochrome and application Hands-on session 3 | Flex Lab A & B |
16:00 – 17:30 | Discussing open questions | Flex Lab A & B |
17:30 – 18:30 | Dinner | EMBL Canteen |
18:30 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | |
08:50 – 09:00 | Arrival and coffee | Flex Lab A & B |
09:00 – 11:30 | Fluorescence microscopy II • Auto-fluorescence – a source for noise • Digital imaging for fluorescence microscopy • Specific needs of digital cameras • Digital multicolor handling • Acquisition of multidimensional images (3D, time) Hands-on session 3 (continued) | Flex Lab A & B |
11:30 – 11:35 | Group picture | Flex Lab A & B |
11:35 – 12:30 | Guided tour of the EMBL Advanced light microscopy facility (ALMF) | ALMF |
12:30 – 13:30 | Lunch | EMBL Canteen |
13:30 – 15:00 | Introduction to the confocal microscope (Talk) | Flex Lab A & B |
15:00 – 18:00 | Hands on session (Group work) Different applications will be covered in small groups. Module 1: Multicolor imaging Module 2: Photomanipulation techniques – FRAP, FLIP and Photoconversion Module 3: 3D image collection and enhancement – zooming, resolution, SNR, z-stacks, IMARIS basic Module 4: Time-lapse imaging, bleaching and viability; Multi-Area Time-Lapses and Stitching | Microscopy rooms |
18:00 – 18:30 | EMBL Bus via ISG Hotel to downtown | ATC Entrance |
18:30 – 20:00 | Gluhwein at the Christmas markets | Weihnachtsmarkt, downtown Heidelberg |
20:00 – 22:00 | Dinner at Kulturbraurei | Christmas markets and then Kulturbraurei |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
08:50 – 09:00 | Arrival and coffee | Flex Lab A & B |
09:00 – 10:00 | FRAP and Photoconversion (Talk) | Flex Lab A & B |
10:00 – 13:00 | Hands-on session (Group work) Work in Modules | Microscopy rooms |
13:00 – 14:00 | Lunch | EMBL Canteen |
14:00 – 17:00 | Hands-on session (Group work) Work in Modules | Microscopy rooms |
17:00 – 18:00 | Comparative time-lapse overnight experiment on three different setups | Microscopy rooms |
18:00 – 19:00 | Dinner | EMBL Canteen |
19:00 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
08:50 – 09:00 | Arrival and coffee | Flex Lab A & B |
09:00 – 09:30 | Evaluation and summary of overnight timelapse acquisition | Flex Lab A & B |
09:30 – 12:30 | Hands on session (Group work) Work in Modules | Microscopy rooms |
12:30 – 13:00 | Tips and tricks for data presentation | Flex Lab A & B |
13:00 – 13:05 | Closing remarks | Flex Lab A & B |
13:05 – 13:15 | Packed lunch | ATC Foyer |
13:15 | EMBL Bus via ISG Hotel to main train station | ATC Main entrance |
The course is limited to 12 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €500 |
PhD Student | €500 |
Industry | €1,000 |
A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively, you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.
Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
– up to €1000 for participants travelling to an EMBL Course from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.
– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.
*Registration fees are only covered for EMBO Practical Courses
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
Applies to selected courses only. Availability will be indicated during the motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 5 nights (Sunday 3rd December – Friday 8th December 2023) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code MIC23-01). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and the location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.
Date: 4 - 8 Dec 2023
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Charlotte Courtney