Christian Eggeling
University of Oxford
UK
Overview
Course Overview
This six day advanced course on fluorescence super-resolution microscopy will cover the theory and practical application of current super-resolution microscopy techniques to biological questions. The participants will have sufficient time to use current super-resolution microscopes for Localisation Microscopy (e.g. dSTORM /GSDIM) and STED Microscopy on a variety of fixed and living samples prepared during the course. Invited guest researchers together with experts from the EMBL and Leica Microsystems will ensure that all aspects are covered in depth.
Audience
This course is aimed at researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level) with access to super-resolution microscopy equipment. The participants should have an immediate need to apply these techniques for their biological questions. The course will strongly focus on practical work and is therefore especially suited for people who rely on advanced microscopy for their research.
Modules/Resources
The course will cover the entire range of skills needed to apply super-resolution microscopy in biology. The following topics will be covered in lectures as well as in practical workshops:
- Sample preparation for super-resolution microscopy
- Multi-Colour 3D STED microscopy
- Multi-Colour 3D Localisation Microscopy
- Data analysis for Localisation Microscopy
- Deconvolution of STED images
- Super-Resolution Imaging of living specimen (STED / PAINT)
Learning Outcomes
At the end of the course, the participants will be able to plan an experiment using super-resolution microscopy, acquire high quality data at the microscope and analyse the images with state-of-the-art computational methods.
This course is organised in cooperation with Leica Microsystems.
Speakers
Speakers
Jan Ellenberg
EMBL Heidelberg
Germany
Mike Heilemann
Goethe University Frankfurt
Germany
Jürgen Reymann
Leica Microsystems CMS GmbH
Germany
Luc Reymond
EPFL Lausanne
Switzerland
Davide Gambarotto
University of Geneva
Switzerland
Giuseppe Vicidomini
IIT Central Research Lab Genova
Italy
Rifka Vlijm
Max Planck Insitute for Medical Research
Germany
Kai Walter
Leica Microsystems CMS GmbH
Germany
Katrin Willig
MPI of Experimental Medicine
Germany
Speakers and Trainers
Francesca Bottanelli
FU Berlin
Germany
Susan Cox
King´s College London
UK
Jana Döhner
University of Zurich
Switzerland
Jens-Peter Gabriel
Leica Microsystems CMS GmbH
Germany
Marko Lampe
Marko Lampe
Germany
Sebastian Schnorrenberg
EMBL Heidelberg
Germany
Ulf Schwarz
Leica Microsystems CMS GmbH
Germany
David Unnersjö Jess
KTH Royal Institute of Technology
Sweden
Trainers
Philipp Hoess, EMBL Heidelberg, Germany
Yiming Li, EMBL Heidelberg, Germany
Ulf Matti, EMBL Heidelberg, Germany
Julia Roberti, Leica Microsystems CMS GmbH, Germany
Falk Schneider, University of Oxford, UK
Erdinc Sezgin, University of Oxford, UK
Christoph Spahn, Johann Wolfgang Goethe-University Frankfurt, Germany
Scientific Organisers
Marko Lampe
EMBL Heidelberg
Germany
Rainer Pepperkok
EMBL Heidelberg
Germany
Ulf Schwarz
Leica Microsystems CMS GmbH
Germany
Course Organisers
Tim Nürnberger
EMBL Heidelberg
Germany
Programme
Date: 8 - 13 Jul 2019
Location: EMBL Heidelberg