Bianka Baying
EMBL Heidelberg
Germany
Overview
Course Overview
The analysis of single cells advances research in many fields. To properly deal with such minute amounts of sample, each step of the workflow is critical, from sampling to data analysis. Additionally, the integration of data from different approaches is becoming increasingly important to capture the whole picture. This course will cover established techniques from sampling through sequencing, protein analysis and data analysis. Samples will be collected via flow cytometry, gene expression will be analysed by qRTPCR and next generation sequencing, and protein analysis will be performed by proximity extension assays. In addition, alternative methods for the whole workflow will be covered in interactive sessions of lectures and Q&A with key experts in the field. Data collected by the different methods will be analysed during in-depth sessions, which are a strong component of the course.
Audience
This course targets young scientists entering the field of single cell analysis and wanting to learn about the benefits and limitations of the approach by performing a complete single cell analysis workflow.
Modules/Resources
- Single cell isolation with FACS
- Single-cell genomics using Strand-Seq
- Single-cell epigenetics using bisulfite sequencing
- Microdroplet-based single-cell transcriptomics
- Single cell NGS data analysis
Learning Outcomes
After finishing the course, attendees will have an overview of available methods in single cell omics and a basic overview of data analysis of single-cell NGS data sets (genomics, epigenetic and transcriptomics). Attendees will also be able to set up some methods in their lab with the knowledge of the detailed protocol and troubleshooting.
For an impression of the course check the 2017 course programme.
Speakers
Speakers & Trainers
Vladimir Benes
EMBL Heidelberg
Germany
Lars Borm
Karolinska Institutet
Sweden
Raffaele Calogero
University of Turin
Italy
Stephen Clark
Babraham Institute
UK
Francesca Cordero
University of Torino
Italy
Qiaolin Deng
Karolinska Institute
Sweden
Eileen Furlong
EMBL Heidelberg
Germany
Caroline Gallant
Uppsala University
Sweden
Karen Grimes
EMBL Heidelberg
Germany
Orane Guillaume-Gentil
ETH Zürich
Switzerland
Patrick Hasenfeld
EMBL Heidelberg
Germany
Susanne Heck
NIHR Biomedical Research Centre
UK
Hoybin Jeong
EMBL Heidelberg
Germany
Davis McCarthy
The University of Melbourne & St Vincent’s Institute of Medical Research
Australia
Katharina Danielski
10x Genomics
Germany
Malte Paulsen
EMBL Heidelberg
Germany
Dinko Pavlinic
EMBL Heidelberg
Germany
David Porubsky
University of Washington
USA
Benjamin Raeder
EMBL Heidelberg
Germany
Ashley Sanders
EMBL Heidelberg
Germany
Daniel Schraivogel
EMBL Heidelberg
Germany
Anders Ståhlberg
Sahlgrenska Academy at University of Gothenburg
Sweden
Oliver Stegle
EMBL-EBI
UK
Sergio Triana
EMBL Heidelberg
Germany
Yvonne Yeboah
EMBL Heidelberg
Germany
Scientific Organisers
Qiaolin Deng
Karolinska Institute
Sweden
Jan Korbel
EMBL Heidelberg
Germany
Anders Ståhlberg
Sahlgrenska Academy at University of Gothenburg
Sweden
Elisabeth Zielonka
EMBL Heidelberg
Germany
Course Organisers
Lea Hohmann
EMBL Heidelberg
Germany
Programme
Date: 12 - 18 May 2019
Location: EMBL Heidelberg