Transgenic Animals – Micromanipulation Techniques

Overview

Course Overview

30% of the course programme covers lectures and tutorials on generating transgenic mice, and corresponding microscopy and micromanipulation techniques by experts in the field. The other 70% focuses on hands-on practical work organised in small groups. These practicals will address injection of nucleic acids into the pronucleus and cytoplasm of fertilised mouse oocytes, embryonic stem cell transfer into blastocysts, piezo-assisted stem cell transfer into 8-cell stage embryos and microscopy training.

Audience

This course is aimed at technicians and scientists interested in the generation of transgenic animals. Participants should have basic experience with eukaryotic cells and microscopy.

Modules/Resources

• DNA/RNA Microinjection into zygotes
• Contrast techniques
• Embryonic stem cell transfer
• Piezo-assisted micromanipulation

Learning Outcomes

The aim of the course is to provide participants with hands-on tutorials and the theory behind generating transgenic animals. Microinjection into mouse zygotes will be covered, as well as cell transfer into embryos and blastocysts. This course is co-organised with Eppendorf.

Speakers

Speakers and trainers

Yvonne Petersen

EMBL Heidelberg

Germany

Sandra Stobrawa

Eppendorf AG

Germany

Frank van der Hoeven

Transgenic Service, German Cancer Research Center

Germany

Werner Wittke

Leica Microsystems CMS GmbH

Germany

Scientific Organisers

Yvonne Petersen

EMBL Heidelberg

Germany

Sandra Stobrawa

Eppendorf AG

Germany

Course Organisers

Maryann Heck

EMBL Heidelberg

Germany

Programme