Justin Barr
Integrated DNA Technologies
USA
Overview
Course Overview
This course will provide hands-on training in genome editing and cell engineering in mammalian cells using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts/knock-ins, and target validation using the most current technologies.
Audience
This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell line using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.
Modules/Resources
During this course you will learn:
- How to design target specific CRISPR/Cas9 tools
- Generation of guide RNA (gRNA)
- Transfection optimization for efficient gene editing
- Generation of knock-out and knock-in cell lines
- Validation of genome-edited cell lines
Learning Outcomes
After this course you should be able to:
- Design CRISPR based editing tools for your target gene of interest
- Choose the right format of gene editing tool and delivery reagent for your cell type of interest
- Edit, screen and validate the engineered cell lines
This course is co-organised by Thermo Fisher.
For an impression of the course check the 2018 course programme.
Speakers
Speakers and Trainers
Vladimir Benes
EMBL Heidelberg
Germany
Silke Deckert
Thermo Fisher Scientific
UK
Victor Dillard
Desktop Genetics
UK
Neil Humphreys
EMBL Rome
Italy
Manuel Kaulich
Goethe University
Germany
Kristof Kersse
Goethe University
Germany
Birgit Koch
Max Planck Institute for Medical Research
Germany
Balca R. Mardin
BioMed X
Germany
Claudio Mussolino
Medical Center – University of Freiburg
Germany
Malte Paulsen
EMBL Heidelberg
Germany
Premnath Rajakannu
Thermo Fisher Scientific
Germany
Kim Remans
EMBL Heidelberg
Germany
Karl von Laer
New England Biolabs
Germany
Sibylle Vonesch
EMBL Heidelberg
Germany
Yvonne Yeboah
EMBL Heidelberg
Germany
Elisabeth Zielonka
EMBL Heidelberg
Germany
Scientific Organisers
Vladimir Benes
EMBL Heidelberg
Germany
Birgit Koch
Max Planck Institute for Medical Research
Germany
Marion Tynan
Thermo Fisher Scientific
UK
Elisabeth Zielonka
EMBL Heidelberg
Germany
Course Organisers
Maryann Heck
EMBL Heidelberg
Germany
Programme
Date: 17 - 22 Mar 2019
Location: EMBL Heidelberg