Digital PCR

Overview

Course Overview

Real-time PCR has been adopted as the gold standard for nucleic acid target quantification in genomics research and is increasingly used for clinical analysis. However, there are some applications, such as precise measurement of copy number variation, low-abundance sequence detection, detection of rare mutations, including distinguishing rare sequences in tumours, and low-level gene expression analysis, where real-time PCR shows limitations. Digital PCR overcomes those limitations by enabling researchers to directly quantify nucleic acids, without the need for a standard curve, and provides higher precision than real-time PCR.

Audience

Researchers entering the field of ddPCR.

Modules/Resources

• ddPCR
• Copy Number Variation
• Rare Event Detection

Learning Outcomes

This course will show you how to plan, perform, and analyse digital PCR experiments and how digital PCR can help your research to overcome the limitations of real-time PCR.

Speakers

Jim Huggett

LGC Group

UK

Mojca Milavec

National Institute of Biology

Slovenia

Valerie Taly

University Paris Descartes

France

Wim Trypsteen

Ghent Universit

Belgium

Jo Vandesompele

Ghent University

Belgium

Alexandra Whale

LGC Group

UK

Scientific Organisers

Jim Huggett

LGC Group

UK

Jo Vandesompele

Ghent University

Belgium

Course Organisers

Maria Mercedes Bacadare Goitia

EMBL Heidelberg

Germany

Programme