Cryo-Electron Microscopy and 3D Image Processing

Overview

Course Overview

Cryo-EM combined with image processing is currently undergoing what has been referred to as a “resolution revolution”, making it one of the most powerful techniques to address this challenge. It also has unique capabilities to obtain structural information in situ within complex environments, and to deal with heterogeneous samples. The methods are complex and under constant development, so in depth, advanced training of users is essential to ensure future users can adapt new technologies to expand into new areas and solve new biological problems. This course provides the theoretical and practical training covering the full breadth of cryo-electron microscopy (cryo-EM) techniques in order develop novel image processing approaches.

Audience

The course is aimed at PhD students and PostDocs who already have experience in cryo-EM and who wish to learn cryo-EM image processing theory and practice with the aim of applying and developing these techniques.

Modules/Resources

• Single particle reconstruction (Imagic, cisTEM, Relion, SPHIRE, cryoSPARCS)
• Subtomogram averaging (Dynamo)
• Single particle helical reconstruction (Spring)
• Atomic model interpretation (Phenix, Refmac and Chimera)
• EM databank

Learning Outcomes

After this course, students should have learned advanced topics of cryo-EM data collection and image processing and be able to apply and adapt these techniques for challenging research projects.

Speakers

Speakers and Trainers

Bettina Böttcher

University of Würzburg

Germany

John Briggs

MRC Laboratory of Molecular Biology

United Kingdom

Radostin Danev

The University of Tokyo

Japan

José Miguel de la Rosa Trevin

SciLifeLab, Stockholm University

Sweden

Tim Grant

HHMI-Janelia Research Campus

USA

Nikolaus Grigorieff

HHMI-Janelia Research Campus

USA

Wim Hagen

EMBL Heidelberg

Germany

Richard Henderson

MRC Laboratory of Molecular Biology

United Kingdom

Arjen Jakobi

Kavli Institute for Nanoscience Delft

The Netherlands

Slavica Jonic

CNRS and Sorbonne Université

France

Jan Kosinski

EMBL Hamburg

Germany

Julia Mahamid

EMBL Heidelberg

Germany

Dustin Morado

MRC Laboratory of Molecular Biology

United Kingdom

Lori Passmore

MRC Laboratory of Molecular Biology

United Kingdom

Pawel A. Penczek

The University of Texas

USA

Paula Pérez Navarro

University of Basel

Switzerland

Jürgen Plitzko

Max Planck Institute for Biochemistry

Germany

Ali Punjani

University of Toronto

Canada

Carsten Sachse

EMBL Heidelberg

Germany

Sara Sandin

Nanyang Technological University

Singapore

Michael Schatz

Image Science

Germany

Sjors Scheres

MRC Laboratory of Molecular Biology

United Kingdom

Henning Stahlberg

University of Basel

Switzerland

Maya Topf

ISMB, Birkbeck, University of London

United Kingdom

Giulia Zanetti

ISMB, Birkbeck, University of London

United Kingdom

Scientific Organisers

Bettina Böttcher

University of Würzburg

Germany

John Briggs

MRC Laboratory of Molecular Biology

United Kingdom

Lori Passmore

MRC Laboratory of Molecular Biology

United Kingdom

Carsten Sachse

EMBL Heidelberg

Germany

Henning Stahlberg

University of Basel

Switzerland

Course Organisers

Christina Dollt

EMBL Heidelberg

Germany

Programme