Quantitative Proteomics: Strategies and Tools to Probe Biology

Overview

Course overview

This course will provide both theoretical and practical training in the use of quantitative proteomics approaches.  Participants will learn the principles of current methodologies including SILAC and TMT as well as targeted proteomics, and they will be trained in the use of bioinformatic tools for data analysis.

Audience

This course is aimed at researchers who know the basics of proteomics and who want to get a deeper understanding of quantitative methodologies, and at those who are familiar with wet-lab proteomic experimentation but want to gain experience in bioinformatic data interpretation. To enter the course a basic understanding of mass spectrometry-based proteomics is beneficial.

Modules/Resources

• The principles of proteome quantification by stable isotope labeling (SILAC and TMT), label-free, and targeted proteomics

• Introduction how various types of mass spectrometers can be used for proteome quantification

• Computer training sessions using software tools for the analysis of various quantitative proteomic data types

• Lectures demonstrating the application of quantitative proteomics in biology, clinical research and drug discovery

Learning outcome

• Understand the principles and use cases of quantitative proteomic methods

• Ability to design quantitative proteomic experiments, including selection of the most appropriate hardware and software

• Ability to analyse quantitative proteomic data by various software tools

Speakers

Speakers

Jürgen Cox

MPI of Biochemistry

Germany

Ileana Cristea

Princeton University

USA

Myriam Demant

Thermo Fisher Scientific

Germany

Ludovic Gillet

ETH Zurich

Switzerland

Anne-Claude Gingras

Lunenfeld-Tanenbaum Research Institute

Canada

Albert Heck

Utrecht University

The Netherlands

Dominic Helm

EMBL Heidelberg

Germany

Bernd Klaus

EMBL Heidelberg

Germany

Jeroen Krijgsveld

DKFZ

Germany

Angus Lamond

University of Dundee

UK

Lennart Martens

Ghent University

Belgium

Shabaz Mohammed

University of Oxford

UK

Paola Picotti

ETH Zurich

Switzerland

Sonja Radau

Thermo Fisher Scientific

Germany

Mikhail Savitski

EMBL Heidelberg

Germany

Tim Van Den Bossche

Ghent University

Belgium

Judit Villen

University of Washington

USA

Trainers

Frank Stein

EMBL Heidelberg

Germany

Scientific Organisers

Jeroen Krijgsveld

DKFZ

Germany

Paola Picotti

ETH Zurich

Switzerland

Mikhail Savitski

EMBL Heidelberg

Germany

Course Organisers

Maria Mercedes Bacadare Goitia

EMBL Heidelberg

Germany

Programme