Next Generation Sequencing: Whole Genome Sequencing Library Preparation

Overview

Course Overview

The goal of this course is to teach the fundamentals of NGS library preparation and validation. We will start with an introduction into NGS technology and the library preparation workflow. The main part will be the preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for whole genome sequencing data analysis.

Audience

This course is directed towards biologists, scientists and technicians who want to apply whole genome sequencing and learn the basics of library preparation. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

During this course we will focus on the following aspects:

• Best practices and tips and tricks for TruSeq DNA PCR-free library preparation
• Best practices of sequencing library validation
• Introduction to whole genome sequencing data analysis

Learning Outcomes

After this course you should be able to:

• Prepare libraries from genomic DNA 
• Quantify and quality control libraries
• Understand how whole genome sequencing data analysis is performed

Speakers

Speakers and Trainers

Nayara Azevedo

EMBL Heidelberg

Germany

Bianka Baying,

EMBL Heidelberg

Germany

Rajna Hercog

EMBL Heidelberg

Germany

Jonathan Landry

EMBL Heidelberg

Germany

Dinko Pavlinic

EMBL Heidelberg

Germany

Jan Provaznik

EMBL Heidelberg

Germany

Tobias Rausch

EMBL Heidelberg

Germany

Ashley Sanders

EMBL Heidelberg

Germany

Björn Textor

NEB (New England Biolabs)

Germany

Laura Villacorta

EMBL Heidelberg

Germany

Scientific Organisers

EMBL Heidelberg

Germany

EMBL Heidelberg

Germany

EMBL Heidelberg

Germany

Course Organisers

EMBL Heidelberg

Germany

Programme