Extracellular Vesicles: From Biology to Biomedical Applications

Overview

Course Overview

A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterization techniques and will address strategies to understand their role in biomedical applications.

Audience

This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.

Modules/Resources

• Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques)
• Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, structural and mechanical characterisation by atomic force microscopy, nanoparticle tracking analysis)
• Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
• Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)

Learning Outcomes

In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.

Speakers

Edit Buzas

Semmelweis University

Hungary

Olivier De Wever

Ghent University

Belgium

Bernd Giebel

Uniklinik Essen

Germany

An Hendrix

Ghent University

Belgium

Cecilia Lässer

Gothenburg University

Sweden

Sten Libregts

Utrecht University

The Netherlands

Suresh Mathivanan

La Trobe University

Australia

Pieter Mestdagh

Ghent University

Belgium

Esther Nolte-’t Hoen

Utrecht University

The Netherlands

Roberta Palmulli

Institut Curie

France

Michiel Pegtel

VU University Medical Center

The Netherlands

Willem Stoorvogel

University of Utrecht

The Netherlands

Johan Swinnen

KU Leuven

Belgium

Jan Van Deun

Ghent University

Belgium

Guillaume Van Niel

Institut Curie

France

Jo Vandesompele

Ghent University

Belgium

Marca Wauben

University of Utrecht

The Netherlands

Scientific Organisers

An Hendrix

Ghent University

Belgium

Esther Nolte-’t Hoen

Utrecht University

The Netherlands

Guillaume Van Niel

Institut Curie

France

Course Organisers

Christina Dollt

EMBL Heidelberg

Germany

Programme