Marco Beccuti
University of Turin
Italy
EMBL Course
Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.
This course will teach the biological researchers how to analyse biological data sets using open-source software. Most of the analysis will be performed with docker4seq package, which was developed to facilitate the use of computing demanding applications in the field of NGS data analysis. Docker4seq package uses docker containers that embed demanding computing tasks (e.g. short reads mapping) into isolated containers. This approach provides multiple advantages: the user does not need to install all the software on its local server, the results generated by different containers can be organised in pipelines and reproducible research is guarantee by the possibility of sharing the docker images used for the analysis.
Computers for hands-on exercises will be provided along with demo data sets.
This course was developed for biological researchers as the intended audience. Basic knowledge of massively parallel sequencing (MPS) is desirable, however R-coding skills and deep knowledge of statistics are not necessary.
“My research focus is on RNA sequencing, infectious diseases and next generation sequencing. Attending this course helped me understand the basics, got me career advice and extended my network. Overall, it made me a better researcher.” Abhay Deep Pandey, Translational Health Science And Technology Institute, India.
“Excellent course on the complete spectrum of RNA-Seq. I would highly recommend this course to all biologists.” Issaac Rajan, King Abdullah University of Science and Technology, Saudi Arabia.
University of Turin
Italy
EMBL Heidelberg
Germany
University of Turin
Italy
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Time | Speaker | Location |
---|---|---|
13:30 | Bus from ISG to EMBL | ISG Hotel entrance |
14:00-14:30 | Arrival, welcome, course overview and safety briefing Vladimir Benes – EMBL Heidelberg, Germany Inna Bösselmann – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany | Courtyard Room A&B |
14:30-15:00 | Participants flash talks | Courtyard Room A&B |
15:00-16:00 | RNA-seq, cool but is it that simple? Vladimir Benes – EMBL Heidelberg, Germany | Courtyard Room A&B |
16:00-16:30 | Coffee Break | Courtyard Foyer |
16:30-18:00 | RNA-seq, cool but is it that simple? Vladimir Benes – EMBL Heidelberg, Germany | Courtyard Room A&B |
18:00-19:00 | Dinner | EMBL Canteen |
19:00 | Shuttle bus to ISG Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG to EMBL | ISG Hotel entrance |
09:00-10:00 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
10:00-10:30 | Coffee Break | ATC Foyer |
10:30-11:00 | Experimental design Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
11:00-12:00 | Docker4seq/rCASC/4SeqGUI overview Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
12:00-13:00 | Lunch | EMBL Canteen |
13:00-13:30 | Docker container and reproducibility Marco Beccuti – University of Turin, Italy | Computer Training Lab |
13:30-14:30 | QC bulk Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
14:30-15:00 | Coffee Break | ATC Foyer |
15:00-16:00 | QC single cells Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
16:00-17:30 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
17:30 – 18:30 | Social programme: Facility tour | |
18:30-20:00 | Dinner | EMBL Canteen |
20:05 | Shuttle bus to ISG Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG to EMBL | ISG Hotel entrance |
09:00-10:30 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
10:30-11:00 | Coffee Break | ATC Foyer |
11:00-12:30 | Counting reads and data reduction Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
12:30-13:30 | Lunch | EMBL Canteen |
13:30-15:00 | Counting reads and data reduction Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
15:00-16:00 | Counting reads miRNA Francesca Cordero – University of Turin, Italy | Computer Training Lab |
16:00-16:30 | Coffee Break | ATC Foyer |
16:30-18:00 | Exercises | Computer Training Lab |
18:00-19:00 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
19:00-20:30 | Dinner | EMBL Canteen |
20:30 | Shuttle bus to ISG Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG to EMBL | ISG Hotel entrance |
09:00-10:00 | Differential expression analysis for bulk RNAseq Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
10:00-11:00 | Sub-population discovery in single cell RNAseq: clustering Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
11:00-11:30 | Coffee Break | ATC Foyer |
11:30-12:30 | Differential expression analysis for multiple groups: ANOVA-like Raffaele Calogero – University of Turin | Computer Training Lab |
12:30-13:30 | Lunch | EMBL Canteen |
13:30-15:00 | Exercises Bulk RNAseq | Computer Training Lab |
15:00-16:00 | Exercises single cell RNAseq | Computer Training Lab |
16:00-16:30 | Coffee Break | ATC Foyer |
16:30-18:00 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
18:10 | Shuttle bus to restaurant | ATC Entrance |
19:30 | Social Dinner at Oskar Restaurant |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG to EMBL | ISG Hotel entrance |
09:00-10:00 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
10:00-11:00 | Mining differential expression results for bulk RNAseq Raffaele Calogero – University of Turin, Italy | Computer Training Lab |
11:00-11:30 | Coffee Break | ATC Foyer |
11:30-12:30 | Depicting clusters specific biomarkers in single cell RNAseq data Francesca Cordero – University of Turin, Italy | Computer Training Lab |
12:30-13:30 | Lunch | EMBL Canteen |
13:30-16:00 | Exercises | Computer Training Lab |
16:00-16:30 | Coffee Break | ATC Foyer |
16:30-17:30 | Exercises | Computer Training Lab |
17:30-18:30 | R pills theory and practice Marco Beccuti – University of Turin, Italy | Computer Training Lab |
18:30-20:00 | Dinner | EMBL Canteen |
20:05 | Shuttle bus to ISG Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG to EMBL | ISG Hotel entrance |
09:00-11:00 | Individual exercises on bulk or single cell RNAseq Raffaele Calogero – University of Turin, Italy Marco Beccuti – University of Turin, Italy | Computer Training Lab |
11:00-11:30 | Coffee Break | ATC Foyer |
11:30-12:30 | Individual exercises on bulk or single cell RNAseq Raffaele Calogero – University of Turin, Italy Marco Beccuti – University of Turin, Italy | Computer Training Lab |
12:30-13:30 | Lunch, farewell and group photo | EMBL Canteen |
13:30-17:00 | Individual exercises on bulk or single cell RNAseq Raffaele Calogero – University of Turin, Italy Marco Beccuti – University of Turin, Italy | Computer Training Lab |
17:00-17:10 | Final remarks Vladimir Benes, Raffaele Calogero, Marco Beccuti | Computer Training Lab |
17:10 | Shuttle bus to Heidelberg Train Station | ATC Entrance |
The course is limited to 16 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration Fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.
Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €690 |
PhD Student | €690 |
Industry | €1100 |
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Registration fee waivers
All academic and student registrants are invited to apply for a registration fee waiver, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO. The registration fee waiver covers the registration sum that you have paid to attend the meeting. Conference participants are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting. If you have already paid the registration fee and are awarded a fee waiver, it will be reimbursed after the meeting. Course participants are required to pay the course fee in advance, which will then be reimbursed after the recipient has attended the course.
Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.
This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
Applications for financial assistance can be submitted via the submission portal* (for the submission of abstracts for conferences or the submission of motivation letters for courses) by completing the Financial Assistance Application Section (underneath the section for entering abstract/motivation letter information). The link to the portal can be found in the registration confirmation email that you will receive after registering for the conference or course.
For conferences, if you are not submitting an abstract, you can still apply for financial assistance in the submission portal. Read the instructions on how to apply for financial assistance.
Note that priority will be given to those submitting an abstract to present at the conference. In your application you will be asked to answer questions regarding your motivation for applying, and, for registration fee waivers, the reasons why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.
The scientific organisers will select the recipients of registration fee waivers during the abstract selection process for conferences and the participant selection process for courses. Results will be announced approximately 3 – 4 weeks before the event start date. Selection results do not impact your admission to the meeting. Registration fee waiver selection is based on your current work or study location, your motivation for applying, the reasons for needing financial support and the impact this event will have on your career. Childcare grants are allocated based on career stage, with priority given to early stage researchers.
Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.
For further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 5 nights (29 May – 3 June 2022) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code DAT22-01). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for onsite events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
Date: 29 May - 3 Jun 2022
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Inna Boesselmann