Jochen Barths
ProSort
France
EMBL Course
Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.
Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. By utilising Flow Cytometry we can identify and quantify specific cell populations from heterogeneous samples, analyse the cell cycle or proliferation state or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2019, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.
The course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry, data acquisition or analysis software (DIVA + FlowJo) is of advantage, but not strictly a must.
Our goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path.
“A comprehensive and interactive course that I would recommend to everyone that uses flow cytometry in their work.” Oana Baldasici, The Oncology Institute Prof. Dr.I. Chiricuta, Romania
ProSort
France
EMBL Heidelberg
Germany
Amsterdam UMC, location VUmc
The Netherlands
(Virtual Speaker)
Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW
Denmark
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
BD
Germany
(Virtual Speaker)
German Cancer Research Center
Germany
Sony Biotechnology Inc.
Germany
Newcastle University
UK
Amsterdam UMC, location VUmc
The Netherlands
EMBL Heidelberg
Germany
Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW
Denmark
German Cancer Research Center
Germany
Course and Conference Team Lead
EMBL Heidelberg
Germany
Training Laboratory Manager
EMBL Heidelberg
Germany
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Time | Speaker | Location |
---|---|---|
08:40 – 09:00 | Bus from ISG to EMBL | Hotel entrance |
09:00 – 10:00 | Welcome, Introduction and Safety briefing | Courtyard Room A & B |
10:00 – 10:45 | Participants flash talks | Courtyard Room A & B |
10:45 – 11:15 | Coffee Break | Flex Lab A & B |
11:15 – 12:00 | Lecture: Fluidics and optics Gelo dela Cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | Courtyard Room A & B |
12:00 – 13:30 | Lunch | EMBL Canteen |
13:30 – 14:15 | Lecture: Fluorescence and PMTs detectors, data generation and data display Steffen Schmitt – German Cancer Research Center, Germany | Courtyard Room A & B |
14:15 – 14:45 | Coffee Break | Flex Lab A & B |
14:45 – 15:30 | Lecture: Data generation and data display Gelo dela Cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | Courtyard Room A & B |
15:30 – 17:15 | Practical 1: Practical Session I: Assessing instrument performance | Training Lab A & B |
17:15 – 17:45 | Coffee break | Flex Lab A & B |
17:45 – 19:30 | Practical Session 2: Absolute counting and proliferation | Training Lab A & B |
19:30 – 21:00 | Dinner | EMBL Canteen |
21:00 – 21:15 | Departure to Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 – 09:00 | Bus from ISG to EMBL | Hotel entrance |
09:00 – 10:00 | Lecture: Fluorescence spillover and compensation Cora Chadick Amsterdam UMC, location VUmc, The Netherlands (Virtual Speaker) | Courtyard Room A & B |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 11:15 | Lecture: Experimental design for multi-color flow Diana Ordonez – EMBL Heidelberg, Germany | Courtyard Room A & B |
11:15 – 12:00 | Lecture: Spectral Flow Cytometry Neda Tafrishi – Sony Biotechnology Inc., Germany | Courtyard Room A & B |
12:00 – 13:30 | Lunch | EMBL Canteen |
13:30 – 15:30 | Practical: Multi- color staining (Group 1) Practical: Data analysis with FlowJo (Group 2) | Group 1: Training Lab A & B Group 2: Courtyard Room A & B |
15:30 – 16:00 | Coffee Break | |
16:00 – 18:00 | Practical: Data analysis with FlowJo (Group 1) Practical: Multi- color staining (Group 2) | Group 1: Courtyard Room A & B Group 2: Training Lab A & B |
18:00 – 19:00 | Lecture: Tips and tricks for intracellular staining Diana Ordonez – EMBL Heidelberg, Germany | Courtyard Room A & B |
19:00 – 19:15 | Departure to Restaurant | ATC Entrance |
19:15 – 21:30 | Dinner | Restaurant |
Time | Speaker | Location |
---|---|---|
08:40 – 09:00 | Bus from ISG to EMBL | Hotel entrance |
09:00 – 10:00 | Lecture: The Essentials of gating, FMO and gating controls Daniel Gimenes – EMBL Heidelberg, Germany | Courtyard Room A & B |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 12:00 | Lecture: Cell Cycle Diana Ordonez – EMBL Heidelberg, Germany | Courtyard Room A & B |
12:00 – 13:30 | Lunch | EMBL Canteen |
13:30 – 13:40 | Group Picture | |
13:40 – 15:40 | Practical: Measuring cell cycle samples (Group 1) Practical: Data analysis with FlowJo (Group 2) | Group 1: Training Lab A & B Group 2: Courtyard Room A & B |
15:40 – 16:10 | Coffee Break | Flex Lab A & B |
16:10 – 18:10 | Practical: Data analysis with FlowJo (Group 1) Practical: Measuring cell cycle samples (Group 2) | Group 1: Courtyard Room A & B Group 2: Training Lab A & B |
18:10 – 18:40 | Q&A Session | Courtyard Room A & B |
18:40 – 20:00 | Dinner | EMBL Canteen |
20:00 | Departure to Hotel | ATC Entrance |
Time | Speaker | Location |
---|---|---|
08:40 – 09:00 | Bus from ISG to EMBL | Hotel entrance |
09:00 – 10:00 | Practical: Data analysis cell cycle- FlowJo | Courtyard Room A & B |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 12:00 | Practical: Data analysis with FlowJo (Users’ data) | Courtyard Room A & B |
12:00 – 13:30 | Lunch | EMBL Canteen |
13:30 – 14:30 | Lecture: Assessing cell death Steffen Schmitt – German Cancer Research Center, Germany | Courtyard Room A & B |
14:30 – 16:00 | Practical: Measuring apoptosis labelling | Training Lab A & B |
16:00 – 16:30 | Coffee Break | Flex Lab A & B |
16:30 – 18:40 | Practical: Data analysis with FlowJo | Courtyard Room A & B |
18:40 | Departure to Hotel | ATC Entrance |
19:00 | Social Activity and Dinner | ISG Hotel |
Time | Speaker | Location |
---|---|---|
08:40 – 09:00 | Bus from ISG to EMBL | Hotel entrance |
09:00 – 10:00 | Lecture: Basics of cell sorting Jochen Barths – ProSort, France | Courtyard Room A & B |
10:00 – 10:30 | Coffee Break | Flex Lab A & B |
10:30 – 12:30 | Practical: Cell sorting and reanalysis of sorted cells | Training Lab A & B |
12:30 – 14:00 | Lunch | EMBL Canteen |
14:00 – 15:00 | Lecture: High-dimensional data analysis with FlowJo Ioannis Panetas – BD, Germany (Virtual Speaker) | Courtyard Room A & B |
15:00 – 15:30 | Feedback and Goodbye | Courtyard Room A & B |
15:30 – 16:00 | Departure to Hotel / Heidelberg Train Station | ATC Entrance |
The course is limited to 12 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration Fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.
Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €600 |
PhD Student | €600 |
Industry | €1100 |
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.
Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course or conference.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.
– up to €1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.
*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.
This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
View our list of external funding opportunities and information on attending a conference as an event reporter.
For further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 6 nights (1 – 6 May 2022) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code CYT22-01). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for onsite events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Sponsorship Opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
Date: 2 - 6 May 2022
Location: EMBL Heidelberg
Deadline(s):
Application: Closed
Organisers:
Contact: Maria Bacadare