Vladimir Benes
EMBL Heidelberg
Germany
EMBO Practical Course
Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.
This course includes a robust theoretical part on the fundamentals of cell sorting delivered by world-leading experts and practical sessions on using standard cuvette sorters. In addition, the course program will include principles, applications, and hands-on sessions on Image Cell Sorting (ICS), allowing attendees to get in touch with this exciting technology!
The course is intended for Ph.D. students and postdocs who are starting to use or already using cell sorting in their research projects. Applicants with previous active knowledge of basic principles of flow cytometry and regular use of flow cytometers in daily research will profit most from the course content.
The topics that will be covered include; instrument set-up and validation, correct operation to avoid technical insufficiency, sample quality, the essentials of gating, performing single-cell sorts for sequencing, and sorting based on morphological traits.
The course will provide knowledge transfer from EMBLs Gene Core regarding single-cell genomics. Our main goal is to provide our attendees with the proper knowledge and experience about cell sorting so that they can go back to their research with high confidence about their sorts.
The principle objectives are to deliver the theory of cell sorting and the practical sides of this high-end technology.
This course is kindly supported by The Flow Cytometry Core Facility at EMBL Heidelberg.
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Cambridge
UK
Amsterdam UMC, location VUmc
The Netherlands
The Francis Crick Institute
UK
Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW
Denmark
EMBL Heidelberg
Germany
Babraham Institute
UK
EMBL Heidelberg
Germany
BD Biosciences
USA
EMBL Heidelberg
Germany
Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW
Denmark
The Francis Crick Institute
UK
Newcastle University
UK
EMBL Heidelberg
Germany
Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW
Denmark
German Cancer Research Center (DKFZ)
Germany
Babraham Institute
UK
Course and Conference Manager
EMBL Heidelberg
Germany
Training Laboratory Manager
EMBL Heidelberg
Germany
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Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
08:55 – 09:10 | Registration | Flex Lab A & B |
09:10 – 09:55 | Welcome, house notes and course overview and Safety Overview | Flex Lab A & B |
09:55 – 10:30 | Flashtalks | Flex Lab A & B |
10:30 – 11:00 | Coffee break | Flex Lab A & B |
11:00 – 11:45 | Lecture: Fluidics and optics Gelo de la cruz – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | Flex Lab A & B |
11:45 – 12:30 | Lecture: Signal Generation Malte Paulsen – Novo Nordisk Foundation Center for Stem Cell Medicine – reNEW, Denmark | Flex Lab A & B |
12:30 – 13:30 | Lunch | Canteen |
13:30 – 14:15 | Lecture: Stream, Nozzles & Drops Derek Davies – Newcastle University, UK | Flex Lab A & B |
14:15 – 15:00 | Lecture: Drop Delay Joana Cerveira – Cambridge University, UK | Flex Lab A & B |
15:00 – 16:30 | Poster session 1 (odd numbers) and coffee break | |
16:30 – 18:30 | Practical session Group 1: Drop delay and Rmax Group 2: Laser alignment All Instructors | Training Lab B |
18:30 – 18:45 | Departure to Hotel | EMBL Bus Stop / ATC Entrance |
18:45 – 19:45 | Dinner | ISG Hotel |
Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:05 – 09:15 | Overview of the day | Flex Lab A & B |
09:15 – 10:00 | Lecture: Cell Sorting Applications Michael Bonadonna – EMBL Heidelberg, Germany | Flex Lab A & B |
10:00 – 10:45 | Lecture: Sample Quality Impact Christopher Hall – Babraham Institute Cambridge University, UK | Flex Lab A & B |
10:45 – 11:15 | Break | Flex Lab A & B |
11:15 – 12:30 | Lecture: The essentials of gating, FMO and gating controls Beata Ramasz – EMBL Heidelberg, Germany | Flex Lab A & B |
12:30 – 13:30 | Lunch | EMBL Canteen |
13:30 – 14:15 | Lecture: Cell Sorting Systems Diana Ordonez – EMBL Heidelberg, Germany | Flex Lab A & B |
14:15 – 15:00 | Lecture: Sorting modes and Index cell sorting Daniel Gimenes – EMBL Heidelberg, Germany | Flex Lab A & B |
15:00 – 16:30 | Poster session 2 (even numbers) and coffee break | |
16:30 – 18:30 | Practical session Group 1: Laser alignment Group 2: Drop delay and Rmax All Instructors | Training Lab B |
18:30 – 19:30 | Dinner | EMBL Canteen |
19:30 – 19:45 | Departure to Hotel | EMBL Bus Stop / ATC Entrance |
Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:05 – 09:15 | Overview of the day | |
09:15 – 10:15 | Lecture: Image cell sorting Aaron Middlebrook – BD Biosciences, USA | Flex Lab A & B |
10:15 – 11:00 | Coffee break and transfers to DKFZ | |
11:00 – 12:30 | Practical Session: Running Different Sorter Types Group 1: Image cell sorter (EMBL) Group 2: Aria Fusion (EMBL) Group 3: Aria Fusion (DKFZ) | Training Lab B |
12:30 – 13:30 | Group Lunch | EMBL/DKFZ Canteen |
13:30 – 18:30 | Practical Session: Running Different Sorter Types Group 1: Image cell sorter (EMBL) Group 2: Aria Fusion (EMBL) Group 3: Aria Fusion (DKFZ) | Training Lab B |
18:30 | Free evening | |
18:30 | Departure to ISG Hotel / Downtown | EMBL Bus Stop / ATC Entrance |
Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:05 – 09:15 | Overview of the day | Flex Lab A & B |
09:15 – 10:00 | Lecture: Quality Single Cell Sorts Cora Chadick – Amsterdam UMC, location VUmc, The Netherlands | Flex Lab A & B |
10:00 – 10:45 | Lecture: Single Cell Genomics Vladimir Benes – EMBL Heidelberg | Flex Lab A & B |
10:45 – 11:30 | Coffee break and transfers to DKFZ | Flex Lab A & B |
11:30 – 13:00 | Practical Session: Running Different Sorter Types Group 1: Aria Fusion (DKFZ) Group 2: Image cell sorter (EMBL) Group 3: Aria Fusion (EMBL) | Training Lab B |
13:00 – 14:00 | Group Lunch | EMBL / DKFZ Canteen |
14:00 – 18:30 | Practical Session: Running Different Sorter Types Group 1: Aria Fusion (DKFZ) Group 2: Image cell sorter (EMBL) Group 3: Aria Fusion (EMBL) | Training Lab B |
18:30 | Departure to Downtown | EMBL Bus Stop / ATC Entrance |
19:00 – 22:00 | Dinner and Bowling |
Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:05 – 09:15 | Overview of the day | Flex Lab A & B |
09:15 – 10:15 | Lecture: Biosafety Considerations Steffen Schmitt – German Cancer Research Center (DKFZ), Germany | Flex Lab A & B |
10:15 – 11:00 | Genomic centre tour & single cell genomics workflow | |
11:00 – 11:30 | Coffee break and transfers to DKFZ | Flex Lab A & B |
11:30 – 13:00 | Practical Session: Running Different Sorter Types Group 1: Aria Fusion (EMBL) Group 2: Aria Fusion (DKFZ) Group 3: Image cell sorter (EMBL) | Training Lab B |
13:00 – 14:00 | Group Lunch | EMBL / DKFZ Canteen |
14:00 – 18:30 | Practical Session: Running Different Sorter Types Group 1: Aria Fusion (EMBL) Group 2: Aria Fusion (DKFZ) Group 3: Image cell sorter (EMBL) | Training Lab B |
18:30 | Departure to Downtown | EMBL Bus Stop / ATC Entrance |
19:00 | Dinner and networking downtown |
Time (Europe/Belin) | Speaker | Location |
---|---|---|
08:40 – 08:55 | Bus from hotel to EMBL ATC Entrance | ISG Hotel |
09:05 – 09:15 | Overview of the day | Flex Lab A & B |
09:15 – 10:30 | Lecture: Tips for publishing high quality cytometry data Derek Davies – The Francis Crick Institute, UK | Flex Lab A & B |
10:30 – 11:00 | Coffee break | Flex Lab A & B |
11:00 – 12:00 | Lecture: Data analysis: Correlating flow and image data Aaron Middlebrook – BD Biosciences, USA | Flex Lab A & B |
12:00 – 13:00 | Feedback and discussion | Flex Lab A & B |
13:00 – 13:15 | Packed lunches and departure | Flex Lab A & B |
13:15 – 13:15 | Departure to Heidelberg Train Station and Downtown | EMBL Bus Stop / ATC Entrance |
The course is limited to 12 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.
This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.
Academia | €400 |
PhD Student | €400 |
Industry | €1000 |
NO visa support letters will be issued until payment of the registration fee is confirmed.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.
Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course or conference.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.
– up to €1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.
*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
For EMBO Practical Course or EMBO Workshop participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course or conference. Eligible costs include fees for a babysitter or child-care facility, travel costs for a caregiver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early-stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant. In order to apply for this grant for EMBO Workshops, you must be registered by the abstract submission deadline.
Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and information on attending a conference as an event reporter.
For further information about financial assistance please refer to the FAQ page.
The registration fee includes accommodation for 6 nights (29 Oct – 4 Nov) at the ISG Hotel in a single room, including breakfast. Any additional nights need to be booked and paid for by you to the hotel. We recommend that all course participants stay at the ISG Hotel, which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Event Supporter:
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
Date: 30 Oct - 4 Nov 2022
Location: EMBL Heidelberg
Deadline(s):
Application: Closed
Organisers:
Contact: Maria Bacadare