Monica Ballarino
Sapienza University of Rome
Italy
EMBO Practical Course
EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.
Over the past decades it became evident that a RNA transcripts specific amount and localisation are important in order to understand the biological function of its (non-) coding gene. Whereby state-of-the-art in situ hybridisation (ISH) technologies are key to visualise these RNAs in a given tissue or cell.
This course aims to highlight three powerful RNA visualisation techniques in histological tissue samples. During this course, the participants will learn to validate the cellular localisation of candidate genes from transcriptome studies through conventional ISH and, the more recent cutting-edge, smFISH (Single Molecule Fluorescent ISH) and HCR-ISH (Hybridization Chain Reaction), based RNA ISH hybridisation methods. Additionally, the course will also cover essential tissue embedding protocols that are adapted for use in visually interrogating RNA transcripts in mammalian tissues.
This course is directed to early stage scientists (PhD and Postdocs) from the fields of RNA and molecular biology as well as various fields with an interest in these methodologies.
Sapienza University of Rome
Italy
EMBL Heidelberg
Germany
Max Delbrück Center for Molecular Medicine
Germany
Centre of Reproductive Medicine and Andrology – University Hospital Münster
Germany
Institute of Molecular Biotechnology
Austria
EMBL Rome
Italy
Friedrich Miescher Institute for Biomedical Research
Switzerland
EMBL Heidelberg
Germany
Institute of Molecular Biotechnology
Austria
EMBL Rome
Italy
EMBL Heidelberg
Germany
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All times in the programme below are shown as the time in Europe/Berlin.
To find out the equivalent time zone in your location, enter Berlin, the programme time and date along with your city into the Time Zone Converter.
Time | Speaker |
---|---|
12:00 – 14:00 | Onboarding and technical troubleshooting |
Time | Speaker |
---|---|
12:45 – 13:00 | Session Chair: Elisabeth Zielonka – EMBL Heidelberg, Germany Check-in and overview of the day |
13:00 – 13:15 | Welcome and house notes and course overview |
13:15 – 14:30 | Icebreaker (e.g. flashtalks) |
14:30 – 14:45 | Break |
14:45 – 15:15 | Live Q&A session based on pre-recorded talk: Histology overview and applications Emerald Perlas – EMBL Rome, Italy |
15:15 – 15:45 | Live Q&A session based on pre-recorded talk : Fluorescent in situ RNA detection techniques Imre Gaspar – Institute of Molecular Biotechnology, Austria |
15:45 – 16:15 | Speed networking break |
16:15 – 17:15 | Student presentations |
Time | Speaker |
---|---|
12:45 – 13:00 | Session Chair: Imre Gaspar – Institute of Molecular Biotechnology, Austria Check-in and overview of the day |
13:00 – 13:30 | Live Q&A Session based on pre-recorded talk mRNA localization and local translation in neurons and neurodegeneration Marina Chekulaeva – Max Delbrück Center for Molecular Medicine, Germany |
13:30 – 14:00 | Live Q&A Session based on pre-recorded talk Probing the pairing paradigm Tim Pollex – EMBL Heidelberg, Germany |
14:00 – 14:15 | Break |
14:15 – 15:00 | Live interactive practical: Sample sectioning live lab demo Emerald Perlas – EMBL Rome, Italy |
15:00 – 15:30 | Break |
15:30 – 16:00 | Live interactive practical continues : Sample sectioning tips and tricks Emerald Perlas – EMBL Rome, Italy |
16:00 – 17:00 | Student presentations |
Time | Speaker |
---|---|
12:45 – 13:00 | Session Chair: Emerald Perlas – EMBL Rome, Italy Check-in and overview of the day |
13:00 – 13:30 | Interactive practical: Imaging conventional ISH- Live Q&A to pre-recorded video Emerald Perlas – EMBL Rome, Italy |
13:30 – 14:00 | Live Q&A Session based on pre-recorded talk Visualizing RNA-protein condensates in the Drosophila female germline Mainak Bose – EMBL Heidelberg, Germany |
14:00 – 14:15 | Break |
14:15 – 15:00 | Interactive practical: Introduction to Probe design- Live Q&A to pre-course assignment Imre Gaspar – Institute of Molecular Biotechnology, Austria |
15:00 – 15:30 | Social networking: Virtual pub quiz |
15:30 – 16:00 | Interactive practical: Introduction to Probe design- Live Q&A to pre-course assignment Imre Gaspar – Institute of Molecular Biotechnology, Austria |
16:00 – 17:00 | Student presentations |
Time | Speaker |
---|---|
12:45 – 13:00 | Session Chair: Imre Gaspar – Institute of Molecular Biotechnology, Austria Check-in and overview of the day |
13:00 – 13:30 | Live Q&A Session based on pre-recorded talk RNA FISH with long probes Ewa Piskadlo – Friedrich Miescher Institute for Biomedical Research, Switzerland |
13:30 – 14:00 | Interactive practical: Single Molecule Fluorescent ISH workflow- FAQs Imre Gaspar – Institute of Molecular Biotechnology, Austria |
14:00 – 14:15 | Break |
14:15 – 15:00 | Interactive practical : Single Molecule Fluorescent ISH Imaging- Demo Imre Gaspar – Institute of Molecular Biotechnology, Austria |
15:00 – 15:30 | Break |
15:30 – 16:00 | Live practical: continues Single Molecule Fluorescent ISH Imaging- Demo Imre Gaspar – Institute of Molecular Biotechnology, Austria |
16:00 – 17:15 | Student presentations |
Time | Speaker |
---|---|
12:45 – 13:00 | Session Chair: Elisabeth Zielonka – EMBL Heidelberg, Germany Check-in and overview of the day |
13:00 – 13:30 | Live Q&A Session based on pre-recorded talk The long non-coding RNA Charme in cardiac and skeletal muscle Monica Ballarino – Sapienza University of Rome, Italy |
13:30 – 14:15 | Interactive practical: Basics ISH image analysis- on provided data set |
14:15 – 14:30 | Break |
14:30 – 15:30 | Live interactive discussion: The solution Room: Experts and peer supported advice on your most pressing problems |
15:30 – 15:45 | Break |
15:45 – 16:15 | Live interactive discussion: The solution Room: Experts and peer supported advice on your most pressing problems |
16:15 – 16:30 | Interactive Session: Course wrap – up session – take away message for participants – short feedback round |
16:30 | Goodbye and end of course |
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to events in Heidelberg and will be indicated during the abstract or motivation letter submission process. A list of external funding opportunities can be found here, and information on attending a conference as an event reporter here.
Onsite events: Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
Virtual events: You are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting.
EMBO childcare grants are available for EMBO Practical Courses and Workshops at all EMBL sites.
The fee waiver will cover the registration sum that you have paid to attend the course.
For EMBO course participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course. Eligible costs include fees for a baby-sitter or child-care facility, travel costs for a care giver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant.
Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.
This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Inhelheim Fonds Fellowship, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
For further information about financial assistance please refer to the FAQ page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course.
While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.
Please do:
Please don’t:
Additional information can be found in our Code of Conduct.
It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the events Slack workspace for you to enjoy during the event.
Questions during and after the talks can be asked in live streaming platform. The chair moderates the questions and shares them with the speaker. If time runs out or you think of a question later, you can use Slack to ask your questions in the dedicated session channels or via direct message.
The programme is planned based on Central European Time (CET), unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accomodate as many timezones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
Date: 26 - 30 Jul 2021
Location: Virtual
Deadline(s):
Closed
Organisers:
Contact: Maryann Heck