In situ structural biology: expanding the toolbox for structural cell biology

Time (Europe/Berlin)	Speaker
12:00 – 13:30	Registration and light refreshments
13:30 – 13:45	Opening remarks
13:45 – 14:45	Keynote lecture Exploring cell biology across scales Jennifer Lippincott-Schwartz – Janelia Research Campus, USA
14:45 – 18:30	Session 1: Molecular machines and macromolecular assemblies
14:45 – 15:00	Stepwise assembly of axonemes visualised through cryo-electron tomography of the ciliary tip. Helen Foster – Human Technopole, Italy
15:00 – 15:15	Explorations with cryo-ET, AI structure prediction, and XL/MS to reveal the molecular components of motile cilia Caitlyn McCafferty – University of Basel, Switzerland
15:15 – 15:30	MINFLUX tracking for monitoring stepping dynamics of kinesin-1 in live cells Takahiro Deguchi – EMBL Heidelberg, Germany
15:30 – 16:00	Biomolecular interactions and structures probed in their physiological environment using site-directed spin labeling electron paramagnetic resonance Enrica Bordignon – University of Geneva. Switzerland
16:00 – 16:30	Coffee Break and Meet the speakers
16:30 – 17:00	Integrative modeling of compositional and conformationally heterogeneous protein assemblies Ignacia Echeverria – University of California, San Francisco, USA
17:00 – 17:15	From 2D live-cell images to a 3D model: how to get structural information in living cells Altair Chinchila Hernandez – Pompeu Fabra University, Spain
17:15 – 17:30	Bridging scales, methods, and expertise: an integrative computational modeling strategy to study structure, dynamics, and function of molecular machines Till Rudack – University of Regensburg, Germany
17:30 – 18:00	How do molecular modules act in concert to generate complex cellular functions? Martin Beck – Max Plank Institute of Biophysics, Germany
18:00 – 18:15	Mechanism of RNA polymerase II elongation complex condensation Richard Stefl – Masaryk University, Czech Republic
18:15 – 18:30	In situ structure and arrangement of the mitochondrial oxidative phosphorylation machinery in whole cells Lea Dietrich – Max Planck Institute for Brain Research, Germany
18:30 – 20:00	Dinner in Canteen
20:00 – 22:00	Pub quiz and after dinner drinks

Time (Europe/Berlin)	Speaker
09:00 – 12:00	Session 2: Novel Technologies 1: advanced imaging
09:00 – 09:30	Combined DNA nanotechnology with single-molecule fluorescence methods for high-resolution microscopy techniques Ralf Jungmann – Max Plank Institute of Biochemistry and LMU, Germany
09:30 – 09:45	Correlating cryo-electron tomography ultrastructure with super-resolution fluorescence imaging using post-acquisition expansion microscopy Nikolai Klena – Human Technopole, Italy
09:45 – 10:00	Registration-free approach for the guidance of cryogenic focused ion beam milling beyond the axial diffraction limit Peter Dahlberg – Stanford University, USA
10:00 – 10:30	Coffee Break and Meet the speakers
10:30 – 11:00	High speed in-liquid atomic force microscopy: direct visualization of proteins in action Toshio Ando – Kanazawa University, Japan
11:00 – 11:15	An optimized cryo-lift-out procedure for studying biomolecular condensates by in situ cryo-electron tomography Gaia Perone – human Technopole, Italy
11:15 – 11:30	Phase contrast in cryo-STEM tomography Peter Kirchweger – Weizmann Institute of Science, Israel
11:30 – 12:00	Imaging intracellular components in situ using superresolution cryo-correlative light and electron microscopy Thom Sharp – University of Bristol, UK
12:00 – 13:30	Vegetarian Lunch and Meet the Editors
13:30-16:30	Session 3: Organelles and Compartments
13:30 – 14:00	High throughput super-resolution microscopy reveals the biophysical principles underlying the dynamic organization of cellular organelles and protein assemblies Suliana Manley – EPL – Switzerland
14:00 – 14:15	Structural elucidation of TORC2 regulation by plasma membrane macrodomains Elda Bauda – University of Geneva, Switzerland
14:15 – 14:30	The nanoscale architecture of ER-LD contact sites suggests a pressure-gated mechanism to control LD growth Veijo Salo – EMBL Heidelberg, Germany
14:30 – 15:00	Coffee break and meet the speakers
15:00 – 15:30	Deciphering the structural mechanisms governing centriole assembly using cryo-electron tomography, expansion and super-resolution microscopy Virginie Hamel – University of Geneva, Swtizerland
15:30 – 15:45	Topography and in-situ structure of neuronal AMPA receptor scaffolding complexes visualized by cryo-electron tomography Richard Held – Stanford University, USA
15:45 – 16:00	Decoding the molecular properties of selective autophagic cargo in situ Javier Lizarrondo Diaz de Cerio – Max Planck Institute of Biophysics, Germany
16:00 – 16:30	Flash talk session 1
16:30 – 18:30	Poster Session 1 (odd numbers)
18:30	Free evening

Time (Europe/Berlin)	Speaker
09:00 – 11:45	Session 4: Single cell systems
09:00 – 09:30	Visualizing the Conformation and Dynamics of FG-Nucleoporins in Live Cells Miao Yu – Wuhan University, China
09:30 – 09:45	Human spermatogenesis leads to a reduced nuclear pore structure and function Alia dos Santos – MRC Laboratory of Molecular Biology, UK
09:45 – 10:00	Capturing endogenous ligands of lipid transfer proteins by native mass spectrometry Carla Kirschbaum, University of Oxford, UK
10:00 – 10:15	Harnessing whole-cell imaging to dissect organelle interdependencies and define cell anatomy: a two-pronged approach Mary Mirvis – University of California, USA
10:15 – 10:45	Coffee break and meet the speakers
10:45 – 11:00	Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons Jana Kroll – Max Delbrück Center, Germany
11:00 – 11:15	CoVET: Cryo-Electron Tomography guided by optical electrophysiology Mingyu Jung – Seoul National University, Republic of Korea
11:15 – 11:30	Visualizing the in-situ structure of tau filaments in an iPSC neurodegenerative disease model Maxwell Tucker – University of California, USA
11:30 – 12:00	Towards cell-scale molecular simulations Siewert-Jan Marrink – University of Groningen, The Netherlands
12:00 – 13:30	Lunch
13:30 – 18:45	Session 5: Cellular Interactions and multicellular systems
13:30 – 14:00	Structural proteomic based on limited proteolysis coupled to mass spectrometry (LiP-MS) with improved, amino acid level-resolution, to probe the mechanism of action of anti- amyloidogenic compounds in situ Tetiana Serdjuk – ETH Zurich, Switzerland
14:00 – 14:15	A generalisable and targeted molecular biopsy approach for in-situ cryo electron tomography of vitreous brain tissue Calina Glynn – Rosalind Franklin Institute, UK
14:15 – 14:30	Genetic and biochemical regulation of crystal morphogenesis in zebrafish iridophores Dvir Gur – Weizmann Institute of Science, Israel
14:30 – 15:00	Coffee break and meet the speakers
15:00 – 15:15	In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-electron tomography Sven Klumpe – Max Planck Institute of Biochemistry, Germany
15:15 – 15:30	In situ studies of plant invasion by fungal pathogens Juan Carlos De la Concepcion – Austrian Academy of Sciences, Austria
15:30 – 16:00	Combining cell biology and electron microscopy, image processing and physical modeling to understand macromolecules in complex scenarios Elizabeth Villa – UCSD, USA
16:00 – 16:45	Flash talk session 2
16:45 – 18:45	Poster session 2 (even numbers)
18:45 – 20:45	Conference dinner in the canteen
20:45 – 00:00	Party

Time (Europe/Berlin)	Speaker
09:00 – 13:00	Session 6: Novel Technologies 2: computational and data integration
09:00 – 09:30	Combining sequence and structure-based methods to predict the impact of protein variants with protein interaction network models of human cell biology Pedro Beltrao – ETH Zurich, Switzerland
09:30 – 09:45	Integrative, dynamic modeling of complex molecular mechanisms: from 3D animation to agent-based simulation Margot Riggi – Max Planck Institute of Biochemistry, Germany
09:45 – 10:15	Developing genetically encoded labels, integrators and recorders for high resolution imaging focusing on Electron Microscopy (EM) methods including correlative cryo-ET Felix Sigmund – Technical University of Munich, Germany
10:15 – 10:30	Surface morphometrics 2.0: mapping local membrane bilayer thickness in the cell Michaela Medina – The Scripps Research Institute, USA
10:30 – 11:00	Coffee break and meet the speakers
11:00 – 11:15	CryoSiam: self-supervised deep learning model for macromolecular identification of in-cell cryo-electron tomography Frosina Stojanovska – EMBL Heidelberg, Germany
11:15 – 11:45	Machine learning methods for computational and structural biology problems with a focus on protein structure determination by cryo-EM/ET Ellen Zhong – Princeton, USA
11:45 – 12:45	Keynote lecture Next generation hardware and software solutions for visual proteomics David Agard – UCSF and CZ Institute for Advanced Biological Imaging, USAp
12:45 – 13:00	Closing remarks and poster prize announcements
13:00	Packed lunch and departure

On-site registration fees include admission, conference materials, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.

Virtual registration fees include access to all of the talks (livestreamed and on demand) and facility to submit questions.

On-site Academia	€450
On-site PhD Student	€350
On-site Industry	€770
On-site Journal Editors*	€300
Virtual Academia	€200
Virtual PhD Student	€150
Virtual Industry	€275

* Editors from scientific journals are allowed to attend at a reduced rate, but asked to contribute to the conference in return by taking part in ‘meet the editors’ sessions or other planned activities within the programme.

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Accredited journalists may be eligible to register for complimentary press registration. Registrants may be required to provide accreditation or equivalent proof of press membership after registration. Please contact Christopher Stocks for more information. Please note that we do not offer complimentary registrations for editors of scientific journals.

Confirmation and payment

Registration will be on a first come, first served basis. Your place can only be confirmed after payment of the registration fee. If you are added to our waiting list, please consider taking advantage of our offerings to participate virtually.

On-site participants: Types of payments accepted are international bank transfers and credit card payments. 

Virtual participants: We are only able to accept card payments. In exceptional cases we can accept bank transfers. Please contact events@embl.de for details.

Abstract submission

Only participants registering to attend the on-site event are eligible to submit an abstract. Abstracts will not be accepted from virtual participants.

After registration you can submit your abstract via a separate link that will be provided in the email confirmation. Alternatively, you can access the link on the confirmation page directly after registering. The same login credentials are used for both processes.

Please note:

Abstract body: The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).

If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.

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Title: The title should not exceed 20 words. Only the first word of the title should start with a capital letter and the rest should be lowercase.

Authors and affiliations: Please fill in the author’s details as requested in the online form. The compulsory fields are: First Name, Last Name, Organisation Name (Affiliation or Company), Country and Email.

Kindly mark only one author in the role of First Author and please don’t forget to indicate who will be the Presenter.

Please enter your co-authors correctly via the system by adding accounts together with their organisation/institute. Do not copy-paste them into the body of the abstract text, as they will not be indexed in the abstract book.

Presentation types: When submitting your abstract, you can apply for an oral or poster presentation. A selection process will take place with the results announced 2-3 weeks after the abstract submission deadline.

For detailed instructions on how to submit a conference abstract, follow the instructions provided in this video.

Please check our FAQs pages for further information on how to submit an abstract.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants, and childcare grants.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course or conference.

Travel grant

The travel grant will cover the cost of travel to an on-site event (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

–up to €400 for participants travelling to an EMBL Conference or EMBO|EMBL Symposium from within Europe.

–up to €1000 for participants travelling to an EMBL Conference or EMBO|EMBL Symposium from outside Europe.

–up to €500 for any participant travelling to an EMBO Workshop.

–up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Workshop.

–up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Workshop.

*Registration fees are only covered for EMBO Workshops

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, and organisers when attending a conference.

Eligible costs include (but are not limited to) fees for a baby-sitter or child-care facility, and travel costs for a caregiver. Please note that priority will be given to early stage researchers. In order to apply for this grant, you must be registered by the abstract submission deadline. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Application

On-site participants

You may apply for financial assistance when submitting your abstract. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

Virtual participants

If you are attending virtually, you can apply for financial assistance in the submission portal by the abstract deadline. Read the instructions on how to apply for financial assistance. Only submissions for financial assistance will be accepted. Presentation abstracts cannot be submitted here and will be declined. 

In your application you will be asked to summarise your current work,  answer questions regarding why your lab cannot fund your attendance, and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

Selection

The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

View our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Accommodation is not included in the conference registration fee.

The hotels below have rooms on hold for participants until 03 January 2025, in some cases at special rates. Please email the hotel directly, quoting the booking code ISS25-01 to confirm the exact price of the room

Hotel Anlage	Hotel Ibis Heidelberg	Premier Inn Heidelberg City
B&B Hotel	Leonardo Hotel Heidelberg City Centre	Hilton Heidelberg
Exzellenz Hotel	Meininger Heidelberg

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Conference shuttle buses

Conference shuttle buses are free of charge for participants, and depart from designated bus stops near the hotels to EMBL and back, mornings and evenings.

View Conference shuttle bus stops and hotels in a larger map. Please note that not every bus stop will be used for every event.

Further details

Address: EMBL, Meyerhofstraße 1, 69117 Heidelberg, Germany.

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

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Charging lockers

There are lockers available next to the stairs leading down into the Auditorium. You will find some of those equipped with sockets to charge your smartphone/tablet etc.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in the Auditorium and all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga and meditation

There is a room for prayer, meditation and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the conference shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Useful German expressions

Hello	Hallo
Goodbye	Auf Wiedersehen (formal) Tschüss (informal)
Good morning	Guten Morgen
Good afternoon	Guten Tag
Good evening	Guten Abend
Good night	Gute Nacht
I’m sorry	Es tut mir leid
Excuse me…	Entschuldigen Sie
How are you?	Wie gehts?
I’m fine thanks. And you?	Mir geht es gut , danke. Und Dir/Ihnen?
What is your name	Wie heißen Sie? (formal) Wie heißt Du? (informal)
My name is	Ich heiße…
Do you speak English	Sprechen Sie englisch?
I don’t understand	Ich verstehe nicht
Please speak more slowly	Können Sie bitte langsamer sprechen
Thank you	Dankeschön
Where is the toilet?	Wo ist die Toilette?
Please call me a taxi	Bitte rufen Sie mir ein Taxi
How do I get to….?	Wie komme ich zum/zur…..?
A beer/two beers please	Ein Bier/zwei Bier bitte
A glass of red/white wine please	Ein Glas Rot/Weisswein bitte
The menu, please	Die Speisekarte, bitte
Is there a local speciality?	Gibt es eine Spezialität aus dieser Gegend?
I’m vegetarian	Ich bin Vegetarier
It was delicious	Es war hervorragend
The bill, please	Die Rechnung, bitte
I have a headache	Ich habe Kopfschmerzen
I have a sore throat	Ich habe Halsschmerzen
My stomach hurts	Ich habe Magenschmerzen
I’m allergic to	Ich bin allergisch gegen
I need a doctor who speaks English	Ich brauche einen Arzt, der englisch spricht

What’s included?

• Access to all the livestreamed talks
• Video library of the recorded talks during the event and for 4 weeks afterwards. Please note: at the request of individual speakers, some talks are not recorded.
• Facility to submit questions

Please note that only on-site participants are able to submit abstracts and participate in the poster sessions.

Event platform

We are using an event platform for this conference. More information about the platform will be shared ahead of the conference.

Guidelines

• Do not broadcast the conference to unregistered participants.
• You are encouraged to tweet and post about the event. Tweet unless the speaker specifically says otherwise, but be mindful of unpublished data. 
• Please do not capture, transmit or redistribute data presented at the meeting.

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the conference platform for you to enjoy during the event.

How to ask questions

Please use the Q&A function in the event platform.

If you have any other questions, you can go to the Help Desk in the event platform. Click on ‘more’ on the top menu and click Help Desk. 

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. Please take your time zone into consideration when planning your attendance.

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for participants page.

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.