The Fundamentals of High-End Cell Sorting

Overview

Course Overview

This course on the fundamentals of high-end cell sorting will enable scientists to properly use the global upstream technology of cell sorting with high confidence. Cell sorting is essential to many studies, but researchers outside of core facilities have very limited knowledge how exactly sorting works and unknowingly produce erroneous samples or even scientifically crippling data.

Audience

The course is intended for PhD students and postdocs who are starting to use, or already using flow cytometry based cell sorting in their research projects. Applicants with some previous active knowledge of basic principles of flow cytometry and a regular use of flow cytometers in daily research will profit most from the content of the course.

Modules/Resources

The topics covered will be the common principles of cell sorting applicable to all droplet sorters, how to properly maintain and evaluate the principle components of a cell sorter and how to correct or avoid technical insufficiency, performing single cell sorts for sequencing or any other common down-stream method. The course will provide knowledge transfer from EMBLs Gene Core with regards to single cell genomics. Our main aim is to provide our attendees with the right knowledge and experience about cell sorting so that they can go back to their research with high confidence about their sorts.

Learning Outcomes

The principle objectives are to deliver the theory of cell sorting as well as the practical sides of this high-end technology.

This course is kindly supported by The Flow Cytometry Core Facility at EMBL Heidelberg.

Speakers

Speakers and Trainers

Attila Bebes

Babraham Institute

UK

Joana Cerveira

University of Cambridge

UK

Cora Chadick

Amsterdam UMC, location VUmc

The Netherlands

Derek Davis

Francis Crick Institute

UK

Diana Ordonez

EMBL Heidelberg

Germany

Malte Paulsen

EMBL Heidelberg

Germany

Beáta Ramasz

EMBL Heidelberg

Germany

Steffen Schmitt

German Cancer Research Center

Germany

Scientific Organisers

Cora Chadick

Amsterdam UMC, location VUmc

The Netherlands

Andrew Filby

Newcastle University, Newcastle upon Tyne

UK

Diana Ordonez

EMBL Heidelberg

Germany

Malte Paulsen

EMBL Heidelberg

Germany

Steffen Schmitt

German Cancer Research Center

Germany

Rachel Walker

Babraham Institute Cambridge University

UK

Course Organisers

Maria Mercedes Bacadare Goitia

EMBL Heidelberg

Germany

Programme