Advances in cryo-electron microscopy and 3D image processing

Time (Europe/Berlin)	Programme	Location
12:00 – 13:30	Virtual onboarding session and introduction to EMBL e-Campus and how to view recorded lectures	Virtual

Time (Europe/Berlin	Programme	Location
15:00 – 15:40	Registration and refreshments	ISG Hotel
15:40 – 15:50	Introduction and housenote slides Sebastian Eustermann – EMBL Heidelberg, Germany	ISG Hotel
15:50 – 16:00	Course overview	ISG Hotel
16:00 – 17:00	Student flash talks	ISG Hotel
17:00 – 17:30	Coffee break	ISG Hotel
17:30 – 18:30	Student flash talks	ISG Hotel
18:30 – 19:45	Remote Lecture 1: Past and future of cryoEM Robert Glaeser – Lawrence Berkeley National Laboratory, USA (virtual speaker)	ISG Hotel
19:45 – 20:45	Dinner	ISG Hotel

Time (Europe/Berlin	Programme	Location
08:40 – 08:45	Overview of the day	Courtyard Rooms
08:45 – 09:05	Industry talk: Automation in cryo-EM workflows Maximilian Gemmer – Thermo Fisher Scientific	Courtyard Rooms
09:05 – 10:20	Lecture: Cryo-EM image formation and optics Chris Russo – MRC Laboratory of Molecular Biology, UK	Courtyard Rooms
10:20 – 10:50	Coffee break kindly sponsored by DECTRIS	Courtyard Rooms
10:50 – 12:05	Lecture: Next generation: microscope hardware Carsten Sachse – Forschungszentrum Jülich, Germany	Courtyard Rooms
12:05 – 13:05	Lunch break	EMBL Canteen
13:05 – 14:20	Remote Lecture: Next generation: cryoEM single particle sample preparation & acquisition Kelly Nguyen – MRC Laboratory of Molecular Biology, UK	Courtyard Rooms
14:20 – 15:35	Lecture: cryoET sample preparation & acquisition Julia Mahamid – EMBL Heidelberg, Germany	Courtyard Rooms
15:35 – 16:05	Coffee break kindly sponsored by DECTRIS	Courtyard Rooms
16:05 – 17:20	Remote Lecture: Fundamentals in image processing: Maximum likelihood Sjors Scheres – MRC Laboratory of Molecular Biology, UK	Courtyard Rooms
17:20 – 18:35	EMBO Young Investigator Lecture: Fundamentals in image processing: cryoET Tanmay Bharat – MRC Laboratory of Molecular Biology, UK	Courtyard Rooms
18:35 – 19:35	Dinner	EMBL Canteen

Time (Europe/Berlin	Programme	Location
08:40 – 08:45	Overview of the day	Courtyard Rooms
08:45 – 09:05	Industry talk Andrew Smith – Kleindiek Nanotechnik, Germany	Courtyard Rooms
09:05 – 10:20	Next generation: High resolution by cryo ET subtomogram averaging Dimitry Tegunov – Genentech, USA	Courtyard Rooms
10:20 – 10:40	Coffee break kindly sponsored by DECTRIS	Courtyard Rooms
10:40 – 11:55	Next generation: 2D template matching and beyond Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Courtyard Rooms
11:55 – 12:25	Panel discussion: Hardware and sample preparation Panel leaders: Simone Mattei and Julia Mahamid – EMBL Heidelberg, Germany	Courtyard Rooms
12:25 – 13:25	Lunch break	EMBL Canteen
13:25 – 15:55	Practical session A Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic Dimitry Tegunov – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation Colin Palmer – UKRI STFC, UK	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B
15:55 – 16:25	Coffee break kindly sponsored by DECTRIS	Courtyard Rooms
16:25 – 18:55	Practical session B Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B
18:55 – 19:55	Canteen dinner	EMBL Canteen
19:55 – 20:55	Remote Lecture: Hot topics: recent breakthroughs and emerging technologies Bridget Carragher – Chan Zuckerberg Imaging Institute, USA	Courtyard Rooms
20:55 – 21:25	Discussion round: Hot topics: recent breakthroughs and emerging technologies	Courtyard Rooms

Time (Europe/Berlin	Programme	Location
09:00 – 09:05	Overview of the day	Courtyard Rooms
09:05 – 10:20	Remote Lecture: Advances in cryoET particle identification Beata Turonova – Max Planck Institute of Biophysics, Germany	Courtyard Rooms
10:20 – 10:40	Coffee break kindly sponsored by SubAngstrom	Courtyard Rooms
10:40 – 11:55	Lecture: AI-based methods: Molecular heterogeneity landscapes from cryoEM and cryoET Rishwanth Raghu – Princeton University, USA	Courtyard Rooms
11:55 – 12:55	Technology Lunch: Complete CryoEM Solutions Mark Ebrahim – SubAngstrom, USA	Rooftop Lounge
12:55 – 13:25	Panel discussion: Software and image processing Panel leaders: Sebastian Eustermann – EMBL Heidelberg, Germany and Tanmay Bharat – MRC Laboratory of Molecular Biology, UK	Courtyard Rooms
13:25 – 15:55	Practical session C Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B
15:55 – 16:25	Coffee break kindly sponsored by SubAngstrom	Courtyard Rooms
16:25 – 18:55	Practical session D Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B
18:55 – 19:55	Pizza dinner	Helix B
19:55 – 20:55	Poster session with drinks (12 posters)	Helix B

Time (Europe/Berlin	Programme	Location
09:00 – 09:05	Overview of the day	Courtyard Rooms
09:05 – 10:05	Lecture: Next generation: Atomic model building, refinement and validation Sebastian Eustermann – EMBL Heidelberg, Germany	Courtyard Rooms
10:05 – 11:05	Lecture: Next generation: integrative model building Jan Kosinski – EMBL Hamburg, Germany	Courtyard Rooms
11:05 – 11:35	Coffee break kindly sponsored by CEOS GmbH	Courtyard Rooms
11:35 – 12:35	EMDB and EMPIAR Data repositories Kyle Morris – EMBL-EBI, UK	Courtyard Rooms
12:35 – 13:05	Panel discussion: Validation and FAIR data Panel leader: Lori Passmore – MRC Laboratory of Molecular Biology, UK and Sebastian Eustermann – EMBL Heidelberg, Germany	Courtyard Rooms
13:05 – 14:05	Technology Lunch: Can aberration correctors help in cryo-electron microscopy? Heiko Müller – CEOS, Germany	Rooftop Lounge
14:05 – 16:35	Practical session E Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
16:35 – 17:05	Coffee break kindly sponsored by CEOS GmbH	Courtyard Rooms
17:05 – 19:35	Practical session F Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
19:35 – 20:35	Dinner	EMBL Canteen
20:35 – 21:35	Poster session with drinks (12 posters)	Helix B

Time (Europe/Berlin	Programme	Location
09:00 – 09:05	Overview of the day	Courtyard Rooms
09:05 – 10:20	High Throughput and Resolution Data collection Simone Mattei – EMBL Heidelberg, Germany	Courtyard Rooms
10:20 – 11:35	Correlative light and electron microscopy for Structural Cell Biology Wanda Kukulski – University of Bern, Switzerland	Courtyard Rooms
11:35 – 11:55	Coffee break kindly sponsored by SubAngstrom	Courtyard Rooms
11:55 – 12:25	Panel discussion: Data acquisition Panel leader: Simone Mattei – EMBL Heidelberg, Germany	Courtyard Rooms
12:25 – 13:25	Lunch break	EMBL Canteen
13:25 – 15:55	Practical Session G Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Colin Palmer – UKRI STFC, UK Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
15:55 – 16:25	Coffee break kindly sponsored by SubAngstrom	Courtyard Rooms
16:25 – 18:55	Practical Session H Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement Colin Palmer – UKRI STFC, UK Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28
18:55 – 19:55	Dinner in EMBL Canteen	EMBL Canteen
19:55 – 20:25	Lecture: cryoEM careers in academia and pharma Dimitry Tegunov – Genentech, USA	Courtyard Rooms
20:25 – 21:25	Discussion round: Career paths	Courtyard Rooms

Time (Europe/Berlin	Programme	Location
10:00 – 10:05	Overview of the day	Courtyard Rooms
10:05 – 12:05	Practical Session I – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Anna Steyer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9 , 10: Imaging Center
12:05 – 13:05	Lunch break	EMBL Canteen
13:05 – 15:05	Practical Session J – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Anna Steyer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9 , 10: Imaging Center
15:05 – 15:25	Coffee break	Courtyard Rooms
15:25 – 17:25	Practical Session K – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany Session 10 on HPF/Waffle prep Anna Steyer – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9 , 10: Imaging Center
17:25 – 19:25	Free time downtown
19:25 – 21:25	Dinner in city centre

Time (Europe/Berlin	Programme	Location
10:00 – 10:05	Overview of the day	Courtyard Rooms
10:05 – 12:05	Practical Session L – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
12:05 – 13:05	Lunch break	EMBL Canteen
13:05 – 15:05	Practical Session M – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL, Germany Session 5 on Model building, refinement and validation (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
15:05 – 15:35	Coffee break	Courtyard Rooms
15:35 – 17:35	Practical Session N – Drop in session Session 1 on Advanced cryoEM single particle analysis | RelionSPA topic (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 2 on Advanced cryoEM single particle analysis | cyroSPARC SPA topic (cont.) Hui Guo – Max Planck Institute of Biochemistry, Germany Session 3 on Advanced cryoET subtomogram averaging | Relion Tomo topic (cont.) Tanmay Bharat – MRC Laboratory of Molecular Biology, UK Andriko von Kügelgen – MRC Laboratory of Molecular Biology, UK Session 4 on Advanced cryoET subtomogram averaging | WARP/M Tomo topic (cont.) Dimitry Tegunov – Genentech, USA Alister Burt – Genentech, USA Joe Dobbs – EMBL Heidelberg, Germany Rasmus Kjeldsen Jensen – EMBL Heidelberg, Germany Session 5 on Model building, refinement and validation (cont.) Sebastian Eustermann – EMBL Heidelberg, Germany Session 6 on Macromolecular Heterogeneity Analysis | cryoDRGN topic (cont.) Ryan Feathers – Princeton University, USA Rishwanth Raghu – Princeton University, USA Session 7 2D template matching (cont.) Nikolaus Grigorieff – University of Massachusetts Medical School, USA Johannes Elferich – University of Massachusetts Medical School, USA Session 8 on Cryo-TEM Simon Fromm – EMBL Heidelberg, Germany Session 9 on Cryo-LM/FIB-SEM Zhengyi Yang – EMBL Heidelberg, Germany	Session 1: Courtyard Rooms Session 2: Flex Lab B Session3: Flex Lab A Session 4: Computer Training Lab Session 5: Helix Seminar Room B Session 6: B11 Session 7: B28 Session 8, 9: Imaging Center
17:35 – 18:50	Poster session	Helix B
18:50 – 19:50	Canteen dinner	EMBL Canteen
19:50 – 20:00	Bus to ISG hotel	ATC Entrance
20:00 – 21:00	Lecture: Future of cell structural biology Julia Mahamid – EMBL Heidelberg, Germany	ISG Hotel

Time (Europe/Berlin	Programme	Location
08:40 – 08:45	Overview of the day	Courtyard Rooms
08:45 – 09:45	Lecture: Hot topics: recent breakthroughs and emerging technologies in structural biology via cryoET Svetlana Dodonova – EMBL Heidelberg, Germany	Courtyard Rooms
09:45 – 10:45	Lecture: Hot topics: recent breakthroughs and emerging technologies in structural biology via cryoEM Cristina Paulino – Heidelberg University, Germany	Courtyard Rooms
10:45 – 11:15	Coffee break	Courtyard Rooms
11:15 – 12:15	Wrap up practicals: highlights presented by participants	Courtyard Rooms
12:15 – 13:15	Final panel discussion: Wrap up with emphasis on the future of cryoEM, responsible practice, reporting and validation standards	Courtyard Rooms
13:15 – 13:30	Packed lunch and departure	ATC Entrance

The course is limited to up to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.

This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue.

Academia	€500
PhD Student	€500
Industry	€1000

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the abstract or motivation letter submission process.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course or conference.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from within Europe.
– up to €1000 for participants travelling to an EMBL Course, EMBL Conference or EMBO|EMBL Symposium from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course or EMBO Workshop.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course or EMBO Workshop.

*Registration fees are only covered for EMBO Practical Courses or EMBO Workshops

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant (EMBO practical courses and workshops only)

For EMBO Practical Course or EMBO Workshop participants with children, there is the possibility to apply for a childcare grant to offset child care costs incurred by participants or speakers when participating at a course or conference. Eligible costs include fees for a baby-sitter or child-care facility, travel costs for a care giver, or travel costs for taking the child to the meeting etc. Please note that priority will be given to early stage researchers. A maximum amount of 500 EUR can be awarded per participant applying for an EMBO Childcare Grant. In order to apply for this grant for EMBO Workshops, you must be registered by the abstract submission deadline.

Application

You may apply for financial assistance when submitting your motivation letter for courses, and abstract for conferences. In your application you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

View our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Hotel

Accommodation in twin rooms, including breakfast, is included in the registration fee. Accommodation has been booked for you at the ISG Hotel for the default dates of 18 to 26 (8 nights). In case you would like to have a single room, please let the hotel know and please note that you will be required to pay an additional cost of 32€ per night (availability of single rooms may be limited). This should be paid by you directly to the ISG during your stay. Any additional nights need to be booked and paid for by you. We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course. 

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

• Map to walk from ISG Hotel to EMBL

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

• ATC orientation map

Please find additional information including FAQs, terms and conditions, COVID-19

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.