Deciphering Methylation: A Novel Enzymatic Approach

Overview

Course Overview

The aim of this course is to prepare and analyze “bisulfite-free” methylome libraries for Illumina sequencing. We will use a novel enzymatic approach, to detect 5-methyl cytosines (5mC’s), that results in longer reads and higher coverage of genomic features at lower minimum coverage thresholds when compared to Illumina bisulfite libraries.

The course participants will be guided through the novel workflow for bisulfite-free library generation. Illumina libraries will be produced using the 50 ng control genomic DNA. Attendees will benefit from hands on experience and gain insight into the method as they progress through the protocol. In addition to the control DNA, participants can also provide a maximum of 2 samples for library preparation, with a required minimum of 100 ng DNA per sample. Libraries will be sequenced, and bioinformatics analysis of a dataset will be discussed.

Audience

This course is directed towards wet-lab biologists, scientists and technicians who want to apply the bisulfite-free method for detecting 5mC’s to their samples. Participants will learn the best practice of library preparation and data analysis. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

– Enzymatic detection of 5mC’s
– NGS
– Introduction to methylome data analysis

Learning Outcomes

Preparation of methylome libraries using an enzymatic approach. Introduction to data analysis of these libraries.

This course is co-organized with NEB

Speakers

Speakers

Vladimir Benes

EMBL Heidelberg

Germany

Arnaud Krebs

EMBL Heidelberg

Germany

Deborah Nörling

Cologne Center for Genomics

Germany

Chaithanya Ponnaluri

New England Biolabs

USA

Björn Textor

New England Biolabs

Germany

Louise Williams

New England Biolabs

USA

Trainers

Ferris Jung

EMBL Heidelberg

Germany

Rozemarijn Kleinendorst

EMBL Heidelberg

Germany

Jonathan Landry

EMBL Heidelberg

Germany

Jan Provaznik

EMBL Heidelberg

Germany

Laura Villacorta

EMBL Heidelberg

Germany

Scientific Organisers

Vladimir Benes

EMBL Heidelberg

Germany

Björn Textor

New England Biolabs

Germany

Course Organisers

Maria Mercedes Bacadare Goitia

EMBL Heidelberg

Germany

Programme