Vladimir Benes
EMBL Heidelberg
Germany
Overview
Course Overview
The aim of this course is to prepare and analyse “bisulfite-free” methylome libraries for Illumina sequencing. We will use a novel enzymatic approach to detect 5-methyl cytosines (5mC’s), that results in longer reads and higher coverage of genomic features at lower minimum coverage thresholds when compared to Illumina bisulfite libraries.
Audience
This course is directed towards wet-lab biologists, scientists and technicians who want to apply the bisulfite-free method for detecting 5mC’s to their samples. Participants will learn the best practice of library preparation and data analysis. Knowledge of next generation sequencing technology is desirable but not essential.
Modules/Resources
- Enzymatic detection of 5mC’s
- NGS
- Introduction to methylome data analysis
Learning Outcomes
Preparation of methylome libraries using an enzymatic approach. Introduction to data analysis of these libraries.
Speakers
Rebecca Berrens
University of Cambridge
UK
Ferris Jung
EMBL Heidelberg
Germany
Arnaud Krebs
EMBL Heidelberg
Germany
Jonathan Landry
EMBL Heidelberg
Germany
Deborah Nörling
University of Bonn
Germany
Mireia Osuna Lopez
EMBL Heidelberg
Germany
Michael Petridis
New England Biolabs GmbH
Germany
Jan Provaznik
EMBL Heidelberg
Germany
Björn Textor
New England Biolabs GmbH
Germany
Louise Williams
GmbH, Germany Williams_Louise Louise Williams New England Biolabs
USA
Scientific Organisers
Vladimir Benes
EMBL Heidelberg,
Germany
Björn Textor
New England Biolabs GmbH
Germany
Course Organisers
Maryann Heck
EMBL Heidelberg
Germany
Programme
Date: 2 - 5 Dec 2019
Location: EMBL Heidelberg