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Imaging down to single-molecule resolution: STED and MINFLUX nanoscopy – Course and Conference Office

EMBL Course

Imaging down to single-molecule resolution: STED and MINFLUX nanoscopy

Overview

Proof of COVID-19 vaccination or recovery is required to attend this on-site course. Please see EMBL’s COVID-19 safety policy for on-site events.

Course overview

The course aims to train biological microscopy users in two forms of super-resolution light microscopy, Stimulated Emission Depletion (STED) Microscopy and Minimal photon fluxes (MINFLUX). Both techniques require technical understanding of the process and instrumentation, training in the method specific requirements of sample preparation and in the acquisition procedures and an understanding of how to further analyze the acquired image data. It is the aim of the course to provide the tools for the successful execution of nanoscopy experiments with these two distinct methods. The course should also allow the participants to understand the specific benefits of the different techniques and thus help them in choosing the right approach for their experimental needs.

Audience

Aimed at early stage researchers (e.g. pre- or postdoctoral level) in life science with access to STED and MINFLUX super-resolution microscopy equipment and at imaging facility staff involved in training and supporting scientists using STED and MINFLUX super-resolution microscopy. Participants should have an immediate need to apply these techniques. The course will have a strong emphasis on practical know-how needed to leverage the latest developments in STED and MINFLUX microscopy. It is therefore equally suited for scientists who already rely on super-resolution microscopy for their research and researchers applying advanced imaging techniques who want to extend their practical knowledge.

Modules/resources

  • Theory and applications of STED and MINFLUX microscopy
  • Sample preparation for STED and MINFLUX super-resolution imaging
  • 2D and 3D STED imaging, multicolour, live cell, tissue and fluorescence lifetime STED imaging
  • 2D and 3D MINFLUX imaging
  • MINFLUX tracking
  • STED and MINFLUX microscopy data analysis

Learning outcome

At the end of the course, participants will be able to plan an experiment relying on STED and MINFLUX super-resolution microscopy, prepare their samples accordingly, record images using optimal conditions and settings and analyse them with appropriate methods and tools.

The EMBL Imaging Centre training activities are generously supported by the Boehringer Ingelheim Foundation.

Speakers

Keynote speaker

Speakers, trainers, and staff

Alf Honigmann

Max Planck Institute

for Molecular Cell

Biology and Genetics

Germany

Stefan Jakobs

Max Planck Institute for

Multidisciplinary

Sciences

and

University Göttingen

Germany

Karine Monier

Institut NeuroMyogène, Physiopathologie and Genetics of Neuron and Muscle

France

Tobias Moser

Max Planck Institute for

Multidisciplinary

Sciences

and

University Göttingen

Germany

Scientific organisers

Course organiser

VIrginia

Virginia Pierini

EMBL Heidelberg

Germany

Programme

Got something to say? Tweet it with #EMBLSTED_MINFLUX

  • Some pre-course work (such as watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 1 – Monday 19 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40Bus from ISG Hotel
09:00 – 09:30 Welcome and House notes
(incl safety)
the Imaging Centre Lecture Hall
09:30 – 09:45Course overviewthe Imaging Centre Lecture Hall
09:45 – 11:00Participant introductionthe Imaging Centre Lecture Hall
11:00 – 11:20Coffee Breakthe Imaging Centre Lecture Hall
11:20 – 12:20Lecture: Overview of Super-resolution Techniques
Marko Lampe – EMBL Heidelberg, Germany
Sebastian Schnorrenberg – EMBL Heidelberg, Germany
the Imaging Centre Lecture Hall
12:20 – 13:05LunchEMBL Canteen
13:05 – 14:05Lecture: Complementary use of STED and MINFLUX to study neuronal synapses
Elisa d’Este – Max Planck Institute for Medical Research, Germany
the Imaging Centre Lecture Hall
14:15 – 16:30Practicals (four in parallel)
Imaging Centre Labs
16:30 – 17:00Coffee Breakthe Imaging Centre Lecture Hall
17:00 – 19:00 Practicals (four in parallel) Imaging Centre Labs
19:00 – 20:00DinnerEMBL Canteen
20:00Departure to Hotel ATC Entrance
Day 2 – Tuesday 20 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40 Bus from ISG Hotel
09:00 – 09:10 Overview of the daythe Imaging Centre Lecture Hall
09:10 – 10:00Lecture: Catching dynamics with reversible switching: From RESOLFT to STARSS
Ilaria Testa – Karolinska Institute of Technology, Sweden
the Imaging Centre Lecture Hall
10:00 – 11:00 Lecture: Labeling biomolecules for live-cell nanoscopy
Grazvydas Lukinavicius – Max Planck Institute for Multidisciplinary Sciences and University Göttingen, Germany
the Imaging Centre Lecture Hall
11:00 – 11:30Coffee Breakthe Imaging Centre Lecture Hall
11:30 – 12:30Lecture: STED & MINFLUX analysis of active zones
Tobias Moser – Max Planck Institute for Multidisciplinary Sciences
and University Göttingen, Germany
the Imaging Centre Lecture Hall
12:30 – 13:30LunchEMBL Canteen
13:30 – 14:30Lecture: Focusing on mitochondria (with nanoscopy)
Stefan Jakobs – Max Planck Institute for Multidisciplinary Sciences and University Göttingen, Germany
the Imaging Centre Lecture Hall
14:30 – 16:30Practicals (four in parallel) Imaging Centre Labs
16:30 – 17:00Coffee Breakthe Imaging Centre Lecture Hall
17:00 – 19:00 Practicals (four in parallel) Imaging Centre Labs
19:00 – 20:00Tips & Tricks with concurrent Pizza DinnerEMBL Canteen
20:00 Departure to HotelATC Entrance
Day 3 – Wednesday 21 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40Bus from ISG Hotel
09:00 – 09:10 Overview of the daythe Imaging Centre Lecture Hall
09:10 – 11:00 Practicals (four in parallel) Imaging Centre Labs
11:00 – 11:30Coffee Breakthe Imaging Centre Lecture Hall
11:30 – 12:30Lecture: STED in Membrane Biology
Alf Honigmann – Max Planck Institute for Molecular Cell Biology and Genetics, Germany
the Imaging Centre Lecture Hall
12:30 – 13:30LunchEMBL Canteen
13:30 – 14:30Lecture: Unraveling cell division by STED: from archaea to human cells
Rifka Vlijm – University of Groningen,
The Netherlands
the Imaging Centre Lecture Hall
14:30 – 15:30Lecture: Super-resolution imaging of nuclear pore complexes
Jan Ellenberg – EMBL Heidelberg, Germany
This lecture will be pre-recorded
the Imaging Centre Lecture Hall
15:30 – 16:00Coffee Break
16:00 – 18:00 Practicals (four in parallel) Imaging Centre Labs
18:00 – 19:00DinnerEMBL Canteen
19:00 – 19:30Departure to HotelATC Entrance
Day 4 – Thursday 22 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40Bus from ISG Hotel
09:00 – 09:10 Overview of the daythe Imaging Centre Lecture Hall
09:10 – 10:00 Practicals (four in parallel) Imaging Centre Labs
11:00 – 11:30Coffee Break the Imaging Centre Lecture Hall
11:30 – 12:30Lecture: Mitotic spindles under the STEDoscope
Kruno Vukušić – Institut Ruđer Bošković, Croatia
the Imaging Centre Lecture Hall
12:30 – 13:30Lunch BreakEMBL Canteen
13:30 – 14:30Lecture: The axon at the nanoscale – challenges and opportunities
Maren Engelhard – Universität Linz,
Austria
the Imaging Centre Lecture Hall
14:30 – 15:00Lecture: Euro-BioImaging: Your gateway to imaging excellence
Antje Keppler – Euro-Bioimaging ERIC, Germany
This lecture will be presented remotely
the Imaging Centre Lecture Hall
15:00 – 15:30Coffee Breakthe Imaging Centre Lecture Hall
15:30 – 17:30 Practicals (four in parallel) Imaging Centre Labs
17:45Return to Hotel
18:40 Social activity in downtown HeidelbergFirebowl
Day 5 – Friday 23 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40Bus from ISG Hotel
09:00 – 09:10 Overview of the daythe Imaging Centre Lecture Hall
09:10 – 11:00Practicals (four in parallel) Imaging Centre Labs
11:00 – 11:30 Coffee Break the Imaging Centre Lecture Hall
11:30 – 12:30 Keynote Lecture: MINFLUXnanoscopy and related matters
Stefan Hell – MPI Göttingen and DKFZ Heidelberg, Germany
the Imaging Centre Lecture Hall
12:30 – 12:45Group photo
12:45 – 13:30LunchEMBL Canteen
13:30 – 14:30Lecture: Live cell STED imaging
Francesca Bottanelli – Freie Universität Berlin, Germany
This lecture will be presented remotely
the Imaging Centre Lecture Hall
14:30 – 15:30Lecture: From SLMS to MINFLUX nanoscopy using cell organelles
Karine Monier – Institut NeuroMyogène, Physiopathologie and Genetics of Neuron and Muscle, France
the Imaging Centre Lecture Hall
15:30 – 16:00Coffee Breakthe Imaging Centre Lecture Hall
16:00 – 18:00Practicals (four in parallel) Imaging Centre Labs
18:00 – 19:30Q&A on STED and MINFLUXthe Imaging Centre Lecture Hall
19:30 – 20:30DinnerEMBL Canteen
20:30Departure to Hotel
Day 6 – Saturday 24 September 2022
Time
(Europe/Berlin)
SpeakerLocation
08:40Bus from ISG Hotel
09:00 – 09:10 Overview of the daythe Imaging Centre Lecture Hall
09:10 – 11:00Practicals (four in parallel) Imaging Centre Labs
11:00 – 11:30Coffee Breakthe Imaging Centre Lecture Hall
11:30 – 12:30Practicals (four in parallel) Imaging Centre Labs
12:30 – 13:30LunchEMBL Canteen
13:30 – 15:00Practicals (four in parallel) Imaging Centre Labs
15:00 – 15:30Coffee Breakthe Imaging Centre Lecture Hall
15:30 – 16:15Final discussion roundthe Imaging Centre Lecture Hall
16:15 – 16:45Feedback round and closing remarksthe Imaging Centre Lecture Hall
17:00Bus to Heidelberg train station and city centreATC Entrance

Practical information

Registration fees and motivation letter

The course is limited to 20 participants (subject to change due to pandemic related capacity restrictions). For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.

Participants are expected to book and pay their own accommodation and travel expenses. 

Academia€500
PhD Student€500
Industry€1100

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by Abberior in the form of registration fee waivers. Availability is limited to participants attending on-site events in Heidelberg and will be indicated during the motivation letter submission process.

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Application

You may apply for financial assistance when submitting your motivation letter. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter or abstract selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection is based on your current work or study location, the reasons for needing financial support and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form has been received.

Further details

See our list of external funding opportunities and information on attending a conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Accommodation and shuttles

Hotel

Single rooms have been reserved for 7 nights (18-25 September 2022) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code EIC22-01). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For further information on getting to EMBL Heidelberg visit our Travel Information page.

For enquiries about accommodation and local transportation please refer to the FAQ page.

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Date: 19 - 24 Sep 2022

Location: EMBL Heidelberg

Venue: EMBL Imaging Centre


Deadline(s):

Application: Closed


Organisers:

  • Marko Lampe
    EMBL Heidelberg, Germany
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