Francesca Bottanelli
Freie Universität Berlin
Germany
EMBL Course
The aim of the course is to train microscopy users in different super-resolution microscopy methods: Stimulated Emission Depletion (STED) and MINFLUX nanoscopy. Both techniques overcome the diffraction limit of light with the help of a donut-shaped laser beam, thereby achieving resolutions that are impossible with conventional light microscopy.
The course combines scientific lectures by experienced researchers from the super-resolution field with hands-on sessions at the different super-resolution microscopes and in the laboratory. Participants will gain a technical understanding of the methods as well as an overview of different application fields and imaging techniques, sample preparation, and data handling for both STED and MINFLUX.
After the course, participants can choose the right approach for their experimental needs and perform their experiments on a STED or MINFLUX microscope.
Aimed at early-stage researchers (e.g. pre- or post-doctoral level) in the life sciences with access to STED and MINFLUX super-resolution microscopy facilities, and at imaging facility staff involved in training and supporting scientists using STED and MINFLUX super-resolution microscopy. Participants should have an immediate need to apply these techniques. The course will have a strong emphasis on the practical know-how needed to exploit the latest developments in STED and MINFLUX microscopy. It is therefore equally suited to scientists who are already using super-resolution microscopy in their research and to researchers using advanced imaging techniques who wish to improve their practical knowledge.
At the end of the course, participants will be able to plan an experiment using STED and MINFLUX super-resolution microscopy, prepare their own samples accordingly, acquire images using optimal conditions and settings and analyse them using appropriate methods and tools.
Please see EMBL’s COVID-19 safety recommendations for testing and other requirements to attend this on-site course.
Freie Universität Berlin
Germany
University Hospital Hamburg Eppendorf
Germany
EMBL Heidelberg
Germany
Max Planck Institute for Biophysical Chemistry
Germany
Johannes Kepler Universität Linz
Austria
Universität Göttingen
Germany
Max Planck Institute for Multidisciplinary Sciences
Germany
Max Planck Institute for Plant Breeding Research
Germany
KTH Royal Institute of Technology
Sweden
University of Vienna
Austria
Freie Universität Berlin
Germany
EMBL Heidelberg
Germany
Max Planck Institute for Multidisciplinary Sciences
Germany
University of Groningen
The Netherlands
Ruđer Bošković Institute
Croatia
Abberior Instruments
Germany
Bioquant Universität Heidelberg
Germany
EMBL Heidelberg
Germany
Abberior Instruments
Germany
Abberior Instruments
Germany
EMBL Heidelberg
Germany
Scientific Training Officer
EMBL Heidelberg
Germany
Course and Conference Officer
EMBL Heidelberg
Germany
Are you on social media? Post using #EMBLSTED_MINFLUX and don’t forget to tag @EMBLEvents.
Time | Description | Location |
---|---|---|
08:40 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:00 – 09:30 | Welcome and house notes (incl safety) | Imaging Center Lecture Hall |
09:30 – 10:00 | Course overview | Imaging Center Lecture Hall |
10:00 – 11:00 | Lecture 1: Overview of Super-resolution Techniques Marko Lampe – DKFZ Heidelberg, Germany + Sebastian Schnorrenberg – EMBL Heidelberg, Germany | Imaging Center Lecture Hall |
11:00 – 11:30 | Coffee Break | Imaging Center Lecture Hall |
11:30 – 12:15 | Lecture 2: What limits meiotic crossovers? (remote) Raphael Mercier – Max Planck Institute for Plant Breeding Research, Germany | Imaging Center Lecture Hall |
12:15 – 13:15 | Lunch | Canteen |
13:15 – 14:15 | Introduction round 1 | Imaging Center Lecture Hall |
14:15 – 14:30 | Coffee break | Imaging Center Lecture Hall |
14:30 – 15:30 | Lecture 3: Imaging the molecular processes of cell division across scales Jan Ellenberg – EMBL Heidelberg, Germany | Imaging Center Lecture Hall |
15:30 – 15:45 | Coffee break | Imaging Center Lecture Hall |
15:45 – 16:30 | Lecture 4: MINFLUX for dynamic structural cell biology (remote) Jonas Ries – University of Vienna, Austria | Imaging Center Lecture Hall |
16:30 – 19:00 | Practical 1 | Imaging Center Labs |
19:00 – 20:00 | Dinner | Canteen |
20:00 | Departure to ISG | ATC Bus Stop |
Time | Description | Location |
---|---|---|
08:40 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:00 – 09:05 | Overview of the day | Imaging Center Lecture Hall |
09:05 – 09:50 | Lecture 5: Unravelling the inner secrets of cells with gene editing and live-cell STED microscopy (remote) Fran Botanelli – Freie Universität Berlin, Germany | Imaging Center Lecture Hall |
09:50 – 10:00 | Coffee Break | Imaging Center Lecture Hall |
10:00 – 12:15 | Introduction round 2 | Imaging Center Lecture Hall |
12:15 – 13:30 | Lunch | Canteen |
13:30 – 16:00 | Practical 2 | Imaging Center Labs |
16:00 – 16:30 | Coffee Break | Imaging Center Lecture Hall |
16:30 – 19:00 | Practical 3 | Imaging Center Labs |
19:00 – 20:00 | Dinner | Canteen |
20:00 | Departure to ISG | ATC Bus Stop |
Time | Description | Location |
---|---|---|
09:30 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:45 – 09:50 | Overview of the day | Imaging Center Lecture Hall |
09:50 – 10:35 | Lecture 7: Sharpening the focus: STED microscopy in unveiling the mysteries of mitotic spindles across species Kruno Vukušić – Ruđer Bošković Institute, Croatia | Imaging Center Lecture Hall |
10:35 – 11:15 | Coffee Break | Imaging Center Lecture Hall |
11:15 – 12:00 | Lecture 8: Optimal STED: from archaea to human cells (remote) Rifka Vlijm – University of Groningen, The Netherlands | Imaging Center Lecture Hall |
12:00 – 12:30 | Lecture 9: Euro-BioImaging ERIC: Opportunities for open access to state-of-the-art imaging expertise and technologies Marianna Childress-Poli – EMBL Heidelberg, Germany | Imaging Center Lecture Hall |
12:30 – 13:30 | Lunch | Canteen |
13:30 – 16:00 | Practical 4 | Imaging Center Labs |
16:00 – 16:30 | Coffee Break | Imaging Center Lecture Hall |
16:30 – 19:00 | Practical 5 | Imaging Center Labs |
19:00 – 20:00 | Dinner | Canteen |
20:00 | Departure to ISG | ATC Bus Stop |
Time | Description | Location |
---|---|---|
08:40 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:00 – 09:05 | Overview of the day | Imaging Center Lecture Hall |
09:05 – 09:50 | Lecture 10: A niche within the niche: Connexins at the AIS Jan Maximilian Janssen – Johannes Kepler University Linz, Austria | Imaging Center Lecture Hall |
09:50 – 10:00 | Coffee Break | Imaging Center Lecture Hall |
10:00 – 12:30 | Practical 6 | Imaging Center Labs |
12:30 – 13:30 | Lunch | Canteen |
13:30 – 16:00 | Practical 7 | Imaging Center Labs |
16:10 | Departure to Heidelberg downtown via ISG Hotel | ATC Bus stop |
17:00 – 21:00 | Course dinner and bowling downtown at Firebowl | Heidelberg downtown |
Time | Description | Location |
---|---|---|
08:40 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:00 – 09:05 | Overview of the day | Imaging Center Lecture Hall |
09:05 – 09:50 | Lecture 11: Charting the nanotopography of inner hair cell ribbon synapses Rohan Kapoor – Universität Göttingen, Germany | Imaging Center Lecture Hall |
09:50 – 10:35 | Lecture 12: Visualizing bacterial infections at the nanoscale Alexander Carsten – Universitätsklinikum Hamburg-Eppendorf, Germany | Imaging Center Lecture Hall |
10:35 – 11:00 | Coffee Break | Imaging Center Lecture Hall |
11:00 – 11:45 | Lecture 13: Exploring Photocontrollable Fluorescent Protein for Live-Cell Imaging at the Nanoscale Francesca Pennacchietti – KTH Royal Institute of Technology, Sweden | Imaging Center Lecture Hall |
11:45 – 12:30 | Lecture 14: Investigating the structure and dynamics of mitochondria at the nanoscale Till Stephan – Max Planck Institute for Multidisciplinary Sciences, Germany | Imaging Center Lecture Hall |
12:30 – 13:20 | Lunch | Canteen |
13:20 – 13:30 | Group photo | in front of the Imaging Center |
13:30 – 14:30 | Keynote Lecture 15: Molecule-scale resolution and dynamics in fluorescence microscopy Stefan Hell – Max Planck Institute for Biophysical Chemistry, Germany | Imaging Center Lecture Hall |
14:30 – 16:30 | Practical 8 | Imaging Center Labs |
16:30 – 16:45 | Coffee Break | Imaging Center Lecture Hall |
16:45 – 19:15 | Practical 9 | Imaging Center Labs |
19:15 – 20:00 | Dinner | Canteen |
20:00 | Departure to ISG | ATC Bus Stop |
Time | Description | Location |
---|---|---|
08:40 | Bus from ISG to EMBL Imaging Center | ISG Hotel |
09:00 – 11:30 | Practical 10 | Imaging Center Labs |
11:30 – 12:30 | Lunch | Canteen |
12:30 – 15:00 | Practical 11 | Imaging Center Labs |
15:00 – 16:00 | Final discussion, feedback round and closing remarks | Imaging Center Lecture Hall |
16:00 | Bus to Heidelberg train station via Hilton hotel | ATC Bus Stop |
The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, meals and coffee. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | € 700 |
PhD Student | € 700 |
Industry | € 1250 |
A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants.
Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
– up to €1000 for participants travelling to an EMBL Course from outside Europe.
*Registration fees are only covered for EMBO Practical Courses
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.
Applies to selected courses only. Availability will be indicated during the motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
Accommodation is not included in the registration fee. Participants are expected to book and pay for their own accommodation. A mix of single and twin rooms have been reserved for 6 nights (20 – 26 October 2024) at ISG Hotel. Single rooms are limited and will be available on a first come, first served basis. After the selection process, selected participants should contact the hotel directly to make a booking with a booking code (code of your course: EIC24-01) by the deadline of 23 September 2024. We recommend that all course participants stay in the dedicated hotel for the course.
For travel information, please see here.
If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.
For more information in English see here or in German see here.
You can book your ticket here.
Shuttle buses will go from ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For more information about accommodation and travel, please refer to the FAQ page.
All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.
Please wear your badge at all times when serving yourself.
No food or drinks are allowed in the laboratories.
In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.
If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.
Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).
Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.
If first aid is required …
In case of fire …
Beyond first aid…
Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.
Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.
Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.
‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.
There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.
There is a nursing room available in the ATC Rooftop Lounge on level A29.
During the course, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.
We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.
There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.
A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.
During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Media partners
Imaging and Microscopy, a Wiley magazine
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
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Date: 21 - 26 Oct 2024
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre and EMBL Imaging Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Karo Wolkersdorfer