Extracellular Vesicles: From Biology to Biomedical Applications

Overview

Course Overview

A rapidly increasing number of researchers aim to purify and identify extracellular vesicles and many methodologies to isolate and analyse these vesicles are available and have been implemented. This course will cover the basics, possibilities and limitations of different purification and characterisation techniques and will address strategies to understand their role in biomedical applications.

Audience

This course is intended for PhD students and Postdoctoral researchers who are interested in learning how to work with extracellular vesicles and know more about their biomedical applications.

Modules/Resources

• Extracellular vesicles (continuous and discontinuous density gradient ultracentrifugation, ultrafiltration techniques, bead-capture and precipitation techniques, size-exclusion chromatography (SEC))
• Extracellular vesicles characterisation (Immunoblot and electron microscopy, fluorescent labeling, quantitative and qualitative analysis by flow cytometry, nanoparticle tracking analysis, in vivo imaging, proteomic and transcriptomic analysis of EV)
• Extracellular vesicles composition (lipidomics, proteomics, transcriptomics)
• Extracellular vesicles and function (molecular players for extracellular vesicle biogenesis, extracellular vesicle targeting to cells, extracellular vesicles as diagnostics and/or therapeutics in pathophysiology)

Learning Outcomes

In this course we intend to make PhD students and post-doctoral researchers familiar with good-practice-guidelines and methods for purification and identification of extracellular vesicles with practical sessions included.

Speakers

Speakers and Trainers

Edit Buzás

Semmelweis University

Hungary

Olivier De Wever

Ghent University

Belgium

Edward Geeurickx

Ghent University

Belgium

Mario Gimona

Paracelsus Medical University

Austria

An Hendrix

Ghent University

Belgium

Cecilia Lässer

University of Gothenburg

Sweden

Estefanía Lozano

Utrecht University

The Netherlands

Suresh Mathivanan

La Trobe University

Australia

Esther Nolte-’t Hoen

Utrecht University

The Netherlands

Roberta Palmulli

Institut Curie

France

Michiel Pegtel

Amsterdam UMC

The Netherlands

Johan Swinnen

KU Leuven

Belgium

Jo Vandesompele

Ghent University

Belgium

Guillaume Van Niel

Institute of Psychiatry and Neurosciences of Paris (IPNP)

France

Marca Wauben

Utrecht University

The Netherlands

Scientific Organisers

An Hendrix

Ghent University

Belgium

Cecilia Lässer

University of Gothenburg

Sweden

Esther Nolte-’t Hoen

Utrecht University

The Netherlands

Guillaume Van Niel

Institute of Psychiatry and Neurosciences of Paris (IPNP)

France

Course Organisers

Christina Dollt

EMBL Heidelberg

Germany

Programme