Genome Engineering: CRISPR / Cas

Overview

Course Overview

This course will provide hands-on training in genome editing and cell engineering in mammalian cells using the highly efficient CRISPR/Cas9 system. Participants will learn design of CRISPR targets using bioinformatics tools, generation of gene knockouts/knock-ins, and target validation using the most current technologies.

Audience

This course is aimed at researchers who are familiar with basic molecular and cell biology techniques and want to learn how to create an engineered mammalian cell line using the most recent and advanced CRISPR/Cas9 system. No previous experience in genome editing is required.

Modules/Resources

During this course you will learn:

• How to design target specific CRISPR/Cas9 tools
• Generation of guide RNA (gRNA)
• Transfection optimization for efficient gene editing
• Generation of knock-out and knock-in cell lines
• Validation of genome-edited cell lines

Learning Outcomes

After this course you should be able to:

• Design CRISPR based editing tools for your target gene of interest
• Choose the right format of gene editing tool and delivery reagent for your cell type of interest
• Edit, screen and validate the engineered cell lines

This course is co-organised by Thermo Fisher.

Speakers

Speakers and Trainers

Justin Barr

Integrated DNA Technologies

USA

Vladimir Benes

EMBL Heidelberg

Germany

Erik Bonke

Promega

Germany

Jonathan D. Chesnut

Thermo Fisher Scientific

USA

Dana Carroll

The University of Utah

USA

Silke Deckert

Thermo Fisher Scientific

UK

Victor Dillard

Desktop Genetics

UK

Neil Humphreys

EMBL Rome

Italy

Kristof Kersse

Thermo Fisher Scientific

UK

Birgit Koch

Max Planck Institute for Medical Research

Germany

Balca R. Mardin

BioMed X

Germany

Claudio Mussolino

Medical Center – University of Freiburg

Germany

Malte Paulsen

EMBL Heidelberg

Germany

Kim Remans

EMBL Heidelberg

Germany

Marion Tynan

Thermo Fisher Scientific

UK

Karl von Laer

New England Biolabs

Germany

Sibylle Vonesch

EMBL Heidelberg

Germany

Ulrike Loewenthal

Thermo Fisher Scientific

UK

Yvonne Yeboah

EMBL Heidelberg

Germany

Elisabeth Zielonka

EMBL Heidelberg

Germany

Scientific Organisers

Vladimir Benes

EMBL Heidelberg

Germany

Birgit Koch

Max Planck Institute for Medical Research

Germany

Marion Tynan

Thermo Fisher Scientific

UK

Elisabeth Zielonka

EMBL Heidelberg

Germany

Course Organisers

George Luca-Ruse

EMBL Heidelberg

Germany

Programme