In situ structural biology: from cryo-EM to multi-scale modelling

Time (Europe/Berlin)	Title
11:30 – 13:00	Registration and light refreshments
13:00 – 13:15	Opening remarks by scientific organisers
13:15 – 14:00	Keynote Rommie Amaro – University of California, USA Introduced by Jan Kosiski
14:00 – 17:00	Session 1: in situ cryo-EM Session chair: Julia Mahamid
14:00 – 14:25	In situ multi-scale structural analysis of virus infection by cellular cryo-tomography Peijun Zhang – University of Oxford, UK **Live stream only**
14:25 – 14:40	Cryo-electron tomography and deep learning denoising reveal native chromatin landscapes of interphase nuclei Mikhail Elfsov – IGBMC, France
14:40 – 14:55	Plate-like guanine biocrystals form via templated nucleation of crystal leaflets on preassembled scaffolds Zohar Eyal – Weizmann Institute of Science, Israel
14:55 – 15:40	Coffee Break and Meet the speakers
15:40 – 16:05	Visualizing ribosome biogenesis in cells with 2DTM Bronwyn Lucas – University of Massachusetts, USA
16:05 – 16:20	Enabling discovery with in-cell cryo-ET & alphaFold: identification of a novel translocation complex Rasmus Jensen – EMBL Heidelberg, Germany
16:20 – 16:35	Structural insight into the Ebola virus nucleocapsid assembly cascade by in-cell cryo-correlative light and electron microscopy Melina Vallbracht – Heidelberg University Hospital, Germany
16:35 – 17:00	Exploring photosynthesis and carbon fixation across scales with cryo-electron tomography Ben Engel – University of Basel, Switzerland
17:00 – 17:40	Flash talks #46 – Gülberk Bayraktar, #48 – Tiana Behr, #52 – Daksh Daksh, # 60 – Chinenye Ekemezie, #74 – Andreja Kezar, # 77 – Matthew Lycas, #82 – Evangelia Nathanail, #84 – Alexander Rau, #85 –  Karolina Roganowicz, #89 –  Simon Schaefer, #94 –  Eva Maria Soriano Jerez, #95 –  Marie-Christin Spindler, #97 –  Reiya Taniguchi, #102 –  Renjing Wang, #103 –  Helena Watson, #106 –  Davide Zabeo, #110 –  Michael Zimmermann,
17:40 – 19:00	Dinner in Canteen
19:00 – 20:30	Welcome reception and tour of Imaging Centre

Time (Europe/Berlin)	Title
08:15 – 09:15	Arrival
09:15 – 12:00	Session 2: Advances in super-resolution light microscopy Session chair: Suliana Manley
09:15 – 09:40	Visualizing the inner life of cells with super-resolution microscopy Melike Lakadamyali – University of Pennsylvania, USA
09:40 – 09:55	4Pi-STORM visualization of nuclear pore complex architecture Mark Bates – Institute for Nanophotonics, Germany **Live stream only**
09:55 – 10:10	Visualization of loop extrusion by nanoscale 3D DNA tracing in single human cells Kai Beckwith – EMBL Heidelberg, Germany
10:10 – 10:40	Coffee Break and Meet the speakers
10:40 – 11:05	Accessing nanoscale structure and dynamics with light Francisco Balzarotti – Research Institute of Molecular Pathology, Austria **Live stream only**
11:05 – 11:20	Toward correlative cryogenic super-resolution light and electron microscopy Hisham Mazal – Max Planck Institute, Germany
11:20 – 11:35	Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis Aline Tschanz – EMBL Heidelberg, Germany
11:35 – 12:00	Bringing light to mitochondrial apoptosis Ana Garcia-Saez – University of Cologne, Germany **Live stream only**
12:00 – 13:30	Lunch, including job fair.
13:30-16:30	Session 3: Modeling across scales Session chair: Rommie Amaro
13:30 – 13:55	Integrative structure modeling of protein complexes in the age of deep learning Dina Schneidman-Duhovny – The Hebrew University of Jerusalem, Israel
13:55 – 14:10	Towards visual proteomics: In-situ detection of macromolecular complexes by high-resolution template matching Sergio Cuz-Leon – Max Planck Institute of Biophysics, Germany
14:10 – 14:25	Combining computation and microscopy to reveal mechanisms in bacterial transport machines Angela Kirykowicz – University of Cambridge, UK
14:25 – 15:10	Coffee break and meet the speakers
15:10 – 15:35	Computational microscopy of biomembrane shapes Weria Pezeshkian – Niels Bohr Institute, Denmark
15:35 – 15:50	Predicting lipid-protein interactions in the age of integrative structural biology Stefano Vanni – University of Fribourg, Switzerland
15:50 – 16:05	Validation of integrative structures in PDB-Dev based on SAXS, CX-MS, and 3DEM data Arthur Zalevsky – University of California, USA
16:05 – 16:30	Animating Data-Rich Molecular Mechanisms Janet Iwasa – University of Utah, USA
16:30 – 17:05	Flash talks #54 –  Sofia De Felice, #55 –  Takahiro Deguchi, #67 –  Jan Philipp Kreysing, #69 –  Nazia Hussain, #79 –  Johannes Rudolph, #87 –  Jenny Sachweh, #101 –  Christian Tueting, #109 –  Liv Zimmermann, #47 –  Paul Becker, #49 –  Harald Bernhard, #51 –  Cristina Capitanio, #93 –  Deniz Daviran, #57 –  Joe Dobbs, #61 –  Bronwen Foster, #73 –  Mukthi Iyer
17:00 – 18:30	Poster Session 1 (odd) with beer and snacks The poster listing can be found here
18:30 – 20:00	Dinner in Canteen
20:00 – 22:00	Pub Quiz and social drinks

Time (Europe/Berlin)	Title
08:15 – 09:15	Arrival
09:15 – 12:00	Session 4: Emerging technologies Session chair: Martin Beck
09:15 – 09:40	What in-cell NMR can teach us about intracellular protein structures and dynamics. Phil Selenko – Weizmann Institute of Science, Israel
09:40 – 09:55	Live-cell imaging and integrative modeling to explore the functionality of protein assemblies Altair Chinchilla Hernandez – Pompeu Fabra University, Spain
09:55 – 10:10	Cryo-EM snapshots of eukaryotic membrane proteins in native lipid-bilayer nanodiscs Kevin Janson – Martin Luther University Halle-Wittenberg, Germany
10:10 – 10:40	Coffee Break and meet the speakers
10:40 – 11:05	Integrated intracellular organization and its variations in human iPS cells  Susanne Rafelski – Allen Institute for Cell Science
11:05 – 11:20	Linking cellular ultrastructure to gene expression with correlative cryo-ET/scRNA-seq Andre Schwarz – Max Planck Institute for Brain Research, Germany
11:20 – 11:35	Exploiting OMVs for the in-situ characterization of bacterial envelope proteins Johannes Thoma – University of Gothenburg, Sweden **live stream only**
11:35 – 12:00	Protein structures in context with proteome-wide biophysics Ilaria Piazza – Max Delbrück Center for Molecular Medicine, Germany **Live stream only**
12:00 – 13:30	Lunch
13:30-16:45	Session 5: Instrumentation Session chair: Jurgen Plitzko
13:30 – 14:15	Keynote The long and winding road to in situ structural biology with cryo-ET Juergen Plitzko – Max Planck Institute of Biochemistry, Germany Introduced by: Julia Mahamid
14:15 – 14:30	Plasma FIB milling for the determination of structures in situ Casper Berger – Rosalind Franklin Institute, UK
14:30 – 14:45	Cryo-FIB milling and cryo-ET of vertically trapped, dividing E. coli bacteria Victoria Hale – MRC Laboratory of Molecular Biology, UK
14:45 – 15:30	Coffee Break and Meet the speakers
15:30 – 15:55	Dynamics of molecules at the nanoscale with photo-switching Ilaria Testa – SciLifeLab, Sweden **remote talk**
15:55 – 16:20	Super-resolved cryogenic correlative light and electron microscopy: Specific labelling with cellular context Peter Dahlberg – Stanford, USA
16:20 – 16:45	Laser phase plate: Advancing beyond proof-of-concept Holger Müller – University of California, Berkeley, USA
16:45 – 17:20	Flash talks #44 – Milan Aleksic #56 –  Natalie Diepenhorst, #58 –  Wioleta Dudka, #62 –  Herman Fung, #66 –  Miguel Hernandez Gonzalez, #76 –  Sven Klumpe, #68 –  Neil Mcdonald, #83 –  Alessandro Passera, #86 –  Max Ruwolt, #88 –  Veijo Salo, #92 –  Ioannis Skalidis, #96 –  Frosina Stojanovska, #100 –  Zuzana Trebichalská, #104 –  Yu-Le Wu, #108 –  Christian Zimmerli
17:20 – 18:45	Poster Session 2 (even) with beer and snacks The poster listing can be found here
18:45 – 20:15	CONFERENCE DINNER in the canteen
20:15 – 22:45	Live music

Time (Europe/Berlin)	Title
08:15 – 09:15	Arrival
09:15 – 12:30	Session 6: Across scales Session chair: Philipp Selenko
09:15 – 09:40	Developing structural interactomics and its application in cell biology Fan Liu – Leibniz-Forschungsinstitut for Molecular Pharmacology, Germany
09:40 – 09:55	Integrative structural studies of thyroid homeostasis: a first look into cellular and organoids organisation Francesca Coscia – Human Technopole, Italy
09:55 – 10:20	Towards automated segmentation in volume electron microscopy Aubrey Weigel – Institute of Genetics and Molecular and Cellular Biology, USA **remote talk**
10:20 – 10:50	Coffee Break and Meet the speakers
10:50 – 11:05	Bacterial nano-compartments, their function in metal homeostasis and role in the cellular response mechanisms against stress Célia Maria Romão – Universidade Nova de Lisboa, Portugal
11:05 – 11:20	How influenza A virus solves the nuclear escape room Daniel Ziemianowicz – EMBL Hamburg, Germany
11:20 – 11:45	Building a mind for cancer Trey Ideker – University of California, USA
11:45 – 12:30	Round table discussion
12:30 – 12:45	Closing remarks and poster prize

Accommodation is not included in the conference registration fee.

As further changes in our events are possible due to COVID-19, you should book flights, trains and hotels with flexible options and favourable cancellation conditions.

Hotel Anlage	Hotel ibis Heidelberg	Premier Inn Heidelberg City
Hotel Bayrischer Hof	ISG Hotel	Staycity Aparthotel Heidelberg
B&B Hotel	Leonardo Hotel Heidelberg City Centre	Steffi’s Hostel Heidelberg
Exzellenz Hotel	Meininger Heidelberg	Hotel Vier Jahreszeiten Heidelberg
Hotel Holländer Hof	Hotel Monpti

Conference shuttle buses

Conference shuttle buses are free of charge for participants, and depart from designated bus stops near the hotels to EMBL and back, mornings and evenings.

The bus stops for this conference are:

• Staycity Aparthotel (Speyerer Str. 7)
• Kurfürsten-Anlage (Opposite Main Train Station)
• Premier Inn (Kurfürsten-Anlage 23)
• Leonardo Hotel Heidelberg City Center (Bergheimer Str. 63)
• Neckarmünzplatz (Heidelberg Tourist Information)
• Peterskirche (Bus stop)
• ISG Hotel (Im Eichwald 19)

View Conference shuttle bus stops and hotels in a larger map. Please note that not every bus stop will be used for every event.

Download the conference bus schedule here. If you miss the bus you can call a taxi at +49 6221 302030

Further details

Address: EMBL, Meyerhofstraße 1, 69117 Heidelberg, Germany. For further information on getting to EMBL Heidelberg visit Public Transportation to the Venue. For information about accommodation and local transportation please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the auditorium.

Charging lockers

There are lockers available next to the stairs leading down into the Auditorium. You will find some of those equipped with sockets to charge your smartphone/tablet etc.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL merchandise

If you are interested in purchasing an EMBL souvenir (products presented in the glass display in the registration area), please ask at the registration desk for more information.

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in the Auditorium and all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available everywhere on the premises (no password required), just log on to ATC-Guest. The eduroam network (secure, world-wide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference an EMBL Photographer may be taking photographs. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help printing your boarding passes/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga and meditation

There is a room for prayer, mediation and yoga located on level E0 behind the Auditorium. Please be respectful of other participants using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the conference shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Useful German expressions

Hello	Hallo
Goodbye	Auf Wiedersehen
Good morning	Guten Morgen
Good afternoon	Guten Tag
Good evening	Guten Abend
Good night	Gute Nacht
I’m sorry	Tut mir leid
Excuse me…	Entschuldigen Sie
How are you?	Wie gehts?
I’m fine thanks. And you?	Mir geht es gut , danke, und dir/Ihnen?
What is your name	Wie heisst du? Wie heissen Sie?
My name is	Ich heisse
Do you speak English	Sprechen Sie Englisch?
I don’t understand	Ich verstehe nicht
Please speak more slowly	Können Sie bitte langsamer sprechen
Thank you	Dankeschön
Where is the toilet?	Wo ist die Toilette?
Please call me a taxi	Bitte rufen Sie mir ein Taxi
How do I get to….?	Wie komme ich zum/zur…..?
A beer/two beers please	Ein Bier/zwei Bier bitte
A glass of red/white wine please	Ein Glas Rot/Weisswein bitte
The menu, please	Die Speisekarte, bitte
Is there a local speciality?	Gibt es eine Spezialität aus dieser Gegend?
I’m Vegetarian	Ich bin Vegetarier
It was delicious	Es war hervorragend
The bill, please	Die Rechnung, bitte
I have a headache	Ich habe Kopfschmerzen
I have a sore throat	Ich habe Halsschmerzen
My stomach hurts	Ich habe Magenschmerzen
I’m allergic to	Ich bin allergisch gegen
I need a doctor who speaks English	Gibt es einen Arzt, der Englisch spricht?

What’s included?

• Access to all the livestreamed talks
• Video library of the recorded talks during and after the event
• Facility to submit questions

Please note that only on-site participants are able to submit abstracts and participate in the poster sessions.

Event platform

We are using an event platform for this conference. More information about the platform will be shared ahead of the conference.

Guidelines

• Do not broadcast the conference to unregistered participants.
• You are encouraged to tweet and post about the event. Tweet unless the speaker specifically says otherwise, but be mindful of unpublished data. 
• Please do not capture, transmit or redistribute data presented at the meeting.

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in the conference platform for you to enjoy during the event.

How to ask questions

Please use the Q&A function in the event platform.

If you have any other questions, you can go to the Help Desk in the event platform. Click on ‘more’ on the top menu and click Help Desk. 

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. Please take your time zone into consideration when planning your attendance.

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.