High-Accuracy CLEM: Applications at Room Temperature and in cryo

Overview

Course Overview

Correlative Light and Electron Microscopy (CLEM) allows precise localisation of an event of interest within cells and tissues. During this course, we will focus on a specific method that has been developed at EMBL that enables the location of fluorescent proteins with high accuracy at the ultrastructural level. The course will cover both the theory and practicals of high precision CLEM, as well as opportunities to discuss their research with CLEM experts and receive practical and troubleshooting advice.

Audience

This course is intended for scientists with experience in EM who want to further develop their skills in high-accuracy correlated microscopy.

Modules/Resources

The course will combine lectures and hands-on practical work covering all steps in the work flow including cryogenic sample preparation, ultramicroscopy, image registration and electron tomography.

Learning Outcomes

Participants will gain hands-on experience with the CLEM methods and learn tips from the people who developed the methods.

Speakers

Speakers and Trainers

Mandy Börmel

EMBL Heidelberg

Germany

Wanda Kukulski

MRC-LMB, Cambridge

United Kingdom

Pedro Machado

EMBL Heidelberg

Germany

Julia Mahamid

EMBL Heidelberg

Germany

Giulia Mizzon

EMBL Heidelberg

Germany

Perrine Paul-Gilloteaux

University of Nantes

France

Paolo Ronchi

EMBL Heidelberg

Germany

Anna Sartori-Rupp

Institut Pasteur

France

Erin Tranfield

Instituto Gulbenkian de Ciência

Portugal

Instituto Gulbenkian de Ciência, Portugal Verkade_Paul Paul Verkade

University of Bristol

UK

Andreas Walter

BioImaging Austria

Austria

Felix Weis

EMBL Heidelberg

Germany

Ievgeniia Zagoriy

EMBL Heidelberg

Germany

Industry Speakers

Jan de Weert

Leica

The Netherlands

Helmut Gnaegi

Diatome Ltd, Nidau,

Switzerland

Alexander Rigort

Thermo Fisher Scientific

The Netherlands

Scientific Organisers

Ori Avinoam

Weizmann Institute of Science

Israel

Rachel Mellwig

EMBL Heidelberg

Germany

Martin Schorb

EMBL Heidelberg

Germany

Course Organisers

Nathalie Sneider

EMBL Heidelberg

Germany

Programme