Ori Avinoam
Weizmann Institute of Science
Israel
EMBO Practical Course
EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.
Correlative Light and Electron Microscopy (CLEM) allows precise localisation of an event of interest within cells and tissues. During this course, we will focus on a specific method that has been developed at EMBL that enables the location of fluorescent proteins with high accuracy at the ultrastructural level. The course will cover both the theory and practicals of high precision CLEM, as well as opportunities to discuss their research with CLEM experts and receive practical and troubleshooting advice.
This course is intended for scientists with experience in EM who want to further develop their skills in high-accuracy correlated microscopy.
The course will combine lectures and hands-on practical work covering all steps in the work flow including cryogenic sample preparation, ultramicroscopy, image registration and electron tomography.
Participants will gain hands-on experience with the CLEM methods and learn tips from the people who developed the methods.
The virtual course has associated pre-course work that needs to be completed before the start of the course. Bear in mind that completing this activity takes time, we recommend to take a look at the materials as soon as you’ve been notified the information is online. Completing this activity takes approximately 7 hours.
The 5-day virtual course in September 2021 includes some live lectures, but will mostly be devoted to hands-on work on real data, in small groups of 4-5 participants.
General notes:
• The virtual course will start on Monday, 30 August, with an onboarding session and access to the pre-recorded lectures and pre-course study materials (exercises, assignments, etc.). It will continue on Monday, 6 September with the regular programme.
• The virtual course includes recorded / live lectures, interactive discussion sessions, online practicals, hands-on exercises, student presentations and networking. • Full-time attendance is required for the course duration.
• Selected participants will have access to the recorded talks 1 week before the start of the event. Please note that watching the recordings prior to the start of the course is required and will take approximately 7 hours.
• Information on the live stream and access to the virtual course platform will be provided shortly before the start of the event.
• Access to the recorded talks and the virtual course platform will be available for 2 weeks after the end of the event.
Weizmann Institute of Science
Israel
Diatome Ltd
Switzerland
King’s College London
UK
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Nantes
France
The Francis Crick Institute
UK
EMBL Heidelberg
Germany
Institut Pasteur
France
EMBL Heidelberg
Germany
Weizmann Institute of Science
Israel
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
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All times in the programme below are shown as the time in Europe/Berlin.
To find out the equivalent time zone in your location, enter Berlin, the programme time and date along with your city into the Time Zone Converter.
Time | Session |
13:00 – 15:00 | Onboarding Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany |
Time | Session |
Introduction | |
12:45 – 13:00 | Overview of the day Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany Elisabeth Zielonka – EMBL Heidelberg, Germany |
13:00 – 13:15 | Welcome, house notes and course overview |
13:15 – 13:45 | Icebreaker activity: Speakers and trainers |
13:45 – 14:30 | Icebreaker activity: Participants part I |
14:30 – 14:45 | Break |
14:45 – 15:30 | Icebreaker activity: Participants part II |
15:30 – 15:45 | Break |
15:45 – 16:45 | Live Q&A session based on pre-recorded talk Paul Verkade – University of Bristol, UK Christopher Peddie – The Francis Crick Institute, UK |
16:45 – 17:00 | Break |
17:00 – 17:45 | Live Q&A session based on pre-recorded talk Paolo Ronchi – EMBL Heidelberg, Germany |
17:45 – 18:15 | Social programme: Speed networking |
Time | Session |
Sample preparation day | |
12:45 – 13:00 | Overview of the day Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany |
13:00 – 13:45 | Live Q&A session based on pre-recorded talk Anna Sartori-Rupp – Institut Pasteur, France |
13:45 – 14:00 | Break |
14:00 – 14:45 | Live Q&A session based on pre-recorded talk Ori Avinoam – Weizmann Institute of Science, Israel |
14:45 – 15:15 | Break with pub quiz |
15:15 – 16:00 | Interactive practical session – HPF and FS Pedro Machado – King’s College London, UK Christopher Peddie – The Francis Crick Institute, UK Rachel Mellwig – EMBL Heidelberg, Germany Ori Avinoam – Weizmann Institute of Science, Israel |
16:00 – 16:15 | Break |
16:15 – 17:00 | Interactive practical session – Plunge freezing Ievgeniia Zagoriy – EMBL Heidelberg, Germany |
17:00 – 17:15 | Break |
17:15 – 19:00 | Interactive troubleshooting session All trainers and speakers |
Break-out rooms |
Time | Session |
Thinning day | |
12:45 – 13:00 | Overview of the day Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany |
13:00 – 14:00 | Live Q&A session based on pre-recorded talk Helmut Gnaegi – Diatome Ltd, Switzerland Viola Oorschot – EMBL Heidelberg, Germany |
14:15 – 15:15 | Interactive practical session – RT sectioning Helmut Gnaegi – Diatome Ltd, Switzerland Viola Oorschot – EMBL Heidelberg, Germany |
15:15 – 15:30 | Break |
15:30 – 16:15 | Live Q&A session based on pre-recorded talk Julia Mahamid – EMBL Heidelberg, Germany |
16:15 – 17:30 | Interactive practical session – cryo thinning Lamella Julia Mahamid – EMBL Heidelberg, Germany Ievgeniia Zagoriy – EMBL Heidelberg, Germany |
17:30 – 17:45 | Break |
17:45 – 18:30 | Social programme: Online Pictionary |
Time | Session |
Acquisition day | |
12:45 – 13:00 | Overview of the day Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany |
13:00 – 14:00 | Interactive practical session – Room Temperature Light Microscopy Giulia Mizzon – EMBL Heidelberg, Germany |
14:00 – 14:15 | Break |
14:15 – 15:45 | Interactive practical session – cryo Light Microscopy Anna Sartori-Rupp – Institut Pasteur, France |
15:45 – 16:00 | Break |
16:00 – 17:00 | Interactive practical session – SerialEM Tutorial Martin Schorb – EMBL Heidelberg, Germany |
17:00 – 17:15 | Break |
17:15 – 19:00 | Interactive troubleshooting session All trainers and speakers |
Time | Session |
Analysis day | |
12:45 – 13:00 | Overview of the day Ori Avinoam – Weizmann Institute of Science, Israel Lea Hohmann – EMBL Heidelberg, Germany Rachel Mellwig – EMBL Heidelberg, Germany Irena Provaznikova – EMBL Heidelberg, Germany Paolo Ronchi – EMBL Heidelberg, Germany Martin Schorb – EMBL Heidelberg, Germany Aline Schnieder – EMBL Heidelberg, Germany |
13:00-13:30 | Live Q&A session to pre-recorded talk Perrine Paul-Gilloteaux – University of Nantes, France |
13:30 – 14:35 | Interactive practical session: SerialEM “practical” Perrine Paul-Gilloteaux – University of Nantes, France Martin Schorb – EMBL Heidelberg, Germany |
14:35 – 14:45 | Live Q&A session to practical session |
14:45 – 15:00 | Break |
15:00-15:30 | |
15:30 – 16:30 | Interactive practical session: ecCLEM Perrine Paul-Gilloteaux – University of Nantes, France Martin Schorb – EMBL Heidelberg, Germany |
16:30 – 16:45 | Break |
16:45 – 17:15 | Interactive practical session: ecCLEM Perrine Paul-Gilloteaux – University of Nantes, France Martin Schorb – EMBL Heidelberg, Germany |
17:15-17:25 | Live Q&A session: practicals (SerialEM and Icy) Perrine Paul-Gilloteaux – University of Nantes, France Martin Schorb – EMBL Heidelberg, Germany |
17:25-18:10 | Interactive practical session: in-depth registration, different software/topics Perrine Paul-Gilloteaux – University of Nantes, France Martin Schorb – EMBL Heidelberg, Germany |
18:10 – 19:00 | Wrap-Up & Farewell |
Event Supporter:
Sponsorship Opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other conferences, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration Fees (include admission and all course materials):
Academia | 125 Euro |
PhD Student | 125 Euro |
Industry | 225 Euro |
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
Motivation letter length: The maximum limit of 2000 characters (ca. 200 words) refers to manually typed text (spaces included).
Free field text length: The maximum characters for your answers in the free field text boxes is 255 (spaces included).Text only: If you copy-paste the text, hidden formatting might still be included and you will be informed that your text exceeds the character limit. We recommend either:
Symbols: If you have special symbols in your text make sure you are using Unicode characters, otherwise these will not be recognised by the tool.
For further information about registration and motivation letter submission please refer to the FAQ page.
Registration Fee Waivers
All academic and student registrants are invited to apply for a registration fee waiver, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO. The registration fee waiver covers the registration sum that you have paid to attend the meeting. Conference participants are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting. If you have already paid the registration fee and are awarded a fee waiver, it will be reimbursed after the meeting. Course participants are required to pay the course fee in advance, which will then be reimbursed after the recipient has attended the course.
For participants and speakers with childcare responsibilities there is the possibility to apply for a grant, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO, to offset childcare costs incurred when participating at a virtual event. Eligible costs include fees for a babysitter or childcare facility or travel costs for a care giver. Please note that priority will be given to early stage researchers. Costs will be reimbursed after the meeting only once a reimbursement form and original receipts have been received. Attendance at the event is required in order to be eligible to receive the reimbursement. In order to apply for this grant, you must be registered by the abstract submission deadline.
Applications for financial assistance can be submitted via the submission portal* (for the submission of abstracts for conferences or the submission of motivation letters for courses) by completing the Financial Assistance Application Section (underneath the section for entering abstract/motivation letter information). The link to the portal can be found in the registration confirmation email that you will receive after registering for the conference or course.
For conferences, if you are not submitting an abstract, you can still apply for financial assistance in the submission portal. Read the instructions on how to apply for financial assistance.
Note that priority will be given to those submitting an abstract to present at the conference. In your application you will be asked to answer questions regarding your motivation for applying, and, for registration fee waivers, the reasons why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.
The scientific organisers will select the recipients of registration fee waivers during the abstract selection process for conferences and the participant selection process for courses. Results will be announced approximately 3 – 4 weeks before the event start date. Selection results do not impact your admission to the meeting. Registration fee waiver selection is based on your current work or study location, your motivation for applying, the reasons for needing financial support and the impact this event will have on your career. Childcare grants are allocated based on career stage, with priority given to early stage researchers.
Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.
For further information about financial assistance please refer to the FAQ page.
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Zoom will be used for the live talks and Q&A sessions. More information will be provided closer to the start of the course
While using the software during the course, please make sure you have nothing else using your bandwidth. We recommend using a wired connection.
Please do:
Please don’t:
Additional information can be found in our Code of Conduct.
It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in eCampus for you to enjoy during the event.
Questions during and after the talks can be asked in the live streaming platform. If time runs out or you think of a question later, you can use the EMBL eCampus forum or send a direct message to the speaker/trainer.
The programme is planned based on the Europe (Berlin, Amsterdam, Paris) time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.
Date: 6 - 10 Sep 2021
Location: Virtual
Deadline(s):
Application: Closed
Organisers:
Contact: Lea Hohmann