Microbial metagenomics: a 360º approach

Time (Europe/ Berlin)	Speaker	Location
16:00 – 17:00	Overview of pre-course work, introduction to course content on EMBL eCampus and troubleshooting Giuseppe D’Auria – FISABIO, Spain	Virtual – Zoom meeting

Time (Europe/ Berlin)	Session	Location
09:00 – 09:30	Welcome, house notes and safety	Courtyard Room A & B
09:30 – 09:45	Welcome and Introductions José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain	Courtyard Room A & B
09:45 – 10:45	Flash talks participants 2 min per participant	Courtyard Room A & B
10:45 – 11:00	Break	Courtyard Room A & B
11:00 – 12:00	Metagenomics: Concepts, historical milestones and next advance José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain	Courtyard Room A & B
12:00 – 13:00	Lunch	EMBL Canteen
13:00 – 14:00	Metagenomics library preparation for NGS Vladimir Benes – EMBL Heidelberg, Germany	Courtyard Room A & B
14:00 – 15:00	Group Activity – Challenges and Expectations	Courtyard Room A & B
15:00 – 16:00	The impact of Metagenomics, on genes and genomes discovery Gabrielle Potock i- Veronese -INRA-LISBP, France	Courtyard Room A & B
16:00 – 16:30	Break	Courtyard Room A & B
16:30 – 17:30	What brings a metagenomic approach in cardiometabolic and nutrition-related disorders? Karine Clément – National Institute for Health and Medical Research (Inserm), France	Courtyard Room A & B
17:30 – 20:00	Poster Session 1 and pizza dinner	ATC Rooftop lounge
20:00	Bus to ISG Hotel	Courtyard Room A & B

Time (Europe/ Berlin)	Session	Location
09:00 – 10:45	Practical: Extraction of metagenomic DNA in groups from soil samples José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
10:45 – 11:15	Break	Courtyard Room A & B
11:15 – 12:45	Practical: Quality control of DNA samples (Gel analysis, Qubit assay) Nanopore 16S PCR set up Mireia Osuna Lopez – EMBL Heidelberg, Germany Hilal Ozgur – EMBL Heidelberg, Germany Laura Villacorta – EMBL Heidelberg, Germany	Flex Lab A & B
12:45 – 13:45	Lunch	EMBL Canteen
13:45 – 14:30	Discussion of metagenomic DNA Extraction Results José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Courtyard Room A & B
14:30 – 15:30	Metagenomics and other culture-independent tools to study virus communities Josefa Antón Botella – University of Alicante, Spain	Courtyard Room A & B
15:30 – 16:45	Practical: Nanopore: 16S PCR cleanup, finishing library preparation and starting sequencing run, followed by Genomics Core Facility Tour Mireia Osuna Lopez – EMBL Heidelberg, Germany Hilal Ozgur – EMBL Heidelberg, Germany Laura Villacorta – EMBL Heidelberg, Germany	Flex Lab A & B
16:45 – 17:15	Break	Courtyard Room A & B
17:15 – 19:15	Practical: Nanopore: 16S analysis/ DNA cleanup and Sequencing start followed by Genomics Core Facility Tour Mireia Osuna Lopez – EMBL Heidelberg, Germany Hilal Ozgur – EMBL Heidelberg, Germany Laura Villacorta – EMBL Heidelberg, Germany	Flex Lab A & B
19:15 – 19:30	Group activity: Description of Case Studies Elisabeth Zielonka- EMBL Heidelberg, Germany José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Giuseppe D’Auria – FISABIO, Spain	Courtyard Room A & B
19:30 – 19:45	Bus to ISG Hotel	EMBL bus stop
20:00	Dinner at ISG Hotel

Time (Europe/ Berlin)	Session	Location
09:00 – 09:45	Practical: Test digestion conditions for metagenomic DNA and preparation of agarose gels José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
09:45 – 10:45	Practical introduction: Single cells genomics in droplets Tobias Wenzel – Pontificia Universidad Católica de Chile, Chile	Flex Lab A & B
10:45 – 11:15	Break	Courtyard Room A & B
11:15 – 11:45	Practical: Loading agarose gels of test-digestion José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
11:45 – 12:45	Lunch	EMBL Canteen
12:45 – 14:15	Practical: Analysis of test-digestion Scale-up of digestion of metagenomic DNA for cloning in the libraries José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
14:15 – 14:45	Break	Courtyard Room A & B
14:45 – 16:45	Practical: Purification from gel of the digested metagenomic DNA Cloning of the metagenomic DNA + overnight ligation Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
16:45 – 17:45	Practical: Preparing bacterial cultures for droplet generation and demonstration of the droplet generation set-up Tobias Wenzel – Pontificia Universidad Católica de Chile, Chile Helper to be confirmed	Flex Lab A & B
17:45 – 18:15	Break	Courtyard Room A & B
18:15 – 19:30	Droplet generation in groups Tobias Wenzel – Pontificia Universidad Católica de Chile, Chile Helper to be confirmed	Flex Lab A & B
19:30 – 20:30	Dinner	EMBL Canteen
20:30	Bus to ISG Hotel	EMBL bus stop

Time (Europe/ Berlin)	Session	Location
09:00 – 11:00	Practical: Inactivation of Ligase, Dialysis, Transformation of E.Coli (Electroporation) José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
11:00 – 12:00	Methodological discussion of practical session 1: Metagenomics experimental design: Common shortcoming and pitfalls José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain	Flex Lab A & B
12:00 – 13:00	Lunch	EMBL Canteen
13:00 – 15:00	Practical: Solving problems and questions: Introduction to Linux and R based on pre-recorded lecture 1 and 2 Giuseppe D’Auria – FISABIO, Spain	Flex Lab A & B
15:00 – 16:00	Metabolomic profiling of microbial communities Mariana Reyes Prieto – FISABIO, Spain	Courtyard Room A & B
16:00 – 16:30	Break	Courtyard Room A & B
16:30 – 17:30	Pacific Bioscience (PacBio) sequencing for metagenomics Vladimir Benes – EMBL Heidelberg, Germany	Courtyard Room A & B
17:30 – 18:00	Bus back to ISG hotel and Downtown	EMBL bus stop
18:00 – 18:30	Free evening with tour of Heidelberg

Time (Europe/ Berlin)	Session	Location
09:00 – 09:45	Practical: Analysis of some colonies from the metagenomic libraries -Interpretation of results José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain Arantxa Lopez – Hospital Can Misses, Spain	Flex Lab A & B
09:45 – 11:45	Practical: Visualization of Droplets Tobias Wenzel – Pontificia Universidad Católica de Chile, Chile Helper to be confirmed	Flex Lab A & B
11:45 – 12:45	Lunch	EMBL Canteen
12:45 – 13:45	Functional and taxonomic analysis with MGnify Kale Varsha- EMBL-EBI, Hinxton, UK	Computer Training Lab
13:45 – 15:45	Practical: Functional and taxonomic analysis with MGnify Kale Varsha- EMBL-EBI, Hinxton, UK	Computer Training Lab
15:45 – 16:15	Break	Courtyard Room A & B
16:15 – 17:00	Metatranscriptomics applied to the study microbial communities José Eduardo González Pastor – Center of Astrobiology (CSIC/INTA), Spain	Courtyard Room A & B
17:00 – 18:00	Pratical: Comparison of sequencing technologies Nanopore, 16S, PacBio Giuseppe D’Auria – FISABIO, Spain	Computer Training Lab
18:00 – 20:00	Poster session with Pizza Dinner	ATC Rooftop lounge
20:00	Bus back to the ISG Hotel	EMBL bus stop

Time (Europe/ Berlin)	Session	Location
09:00 – 12:30	Introduction to 16S rDNA profiling (Illumina/PacBio) Practical 6: 16S rDNA profiling (Illumina/PacBio) Giuseppe D’Auria – FISABIO, Spain Flexible break	Computer Training Lab
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 14:45	Practical: Practice to 16S rDNA profiling (Illumina/PacBio) Giuseppe D’Auria – FISABIO, Spain	Computer Training Lab
14:45 – 15:15	Break	Courtyard Room A & B
15:15 – 17:00	Practical: Genome-Resolved Metagenomic Kostas Konstantinidis – Georgia Institute of Technology, USA Luis-Miguel Rodriguez-R- University of Innsbruck, Austria	Computer Training Lab
17:00 – 17:30	Break	Courtyard Room A & B
17:30 – 19:30	Practical: Genome-Resolved Metagenomic Kostas Konstantinidis – Georgia Institute of Technology, USA Luis-Miguel Rodriguez-R University of Innsbruck, Austria	Computer Training Lab
19:30 – 20:30	Dinner	EMBL Canteen
20:30	Bus back to the ISG Hotel

Time (Europe/ Berlin)	Session	Location
09:00 – 11:15	Practical: Metagenomic assembly, ORF finding, functional annotation. Giuseppe D’Auria – FISABIO, Spain	Computer Training Lab
11:15 – 11:30	Break	Courtyard Room A & B
11:30 – 12:30	Analysis Tools Overview: INCA, MILC and MELP Kristian Vlahovicek – University of Zagreb, Croatia	Computer Training Lab
12:30 – 13:30	Lunch	EMBL Canteen
13:30 – 15:30	Practical: INCA Kristian Vlahovicek – University of Zagreb, Croatia	Computer Training Lab
15:30 – 16:00	Break	Courtyard Room A & B
16:00 – 18:00	Student presentation preparation time: Case Studies All trainers and speakers	Courtyard Room A & B
18:00 – 19:00	Bus Downtown	EMBL bus stop
19:00 – 22:00	Dinner Downtown
22:00	Bus back to the ISG Hotel

Time (Europe/ Berlin)	Session	Location
09:45 – 11:45	Practical: Metagenomic analysis using DIAMOND+MEGAN Daniel Huson – University of Tübingen, Germany	Computer Training Lab
11:45 – 12:45	Lunch	EMBL Canteen
12:45 – 13:45	Practical: Metagenomic analysis using DIAMOND+MEGAN Daniel Huson – University of Tübingen, Germany	Computer Training Lab
13:45 – 15:15	Group activity and presentations: Case Studies	Courtyard Room A & B
15:15 – 15:45	Wrap-up and conclusions	Courtyard Room A & B
15:45 – 16:00	Bust to Heidelberg train station and Downtown	EMBL bus stop

The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, meals and coffee breaks. This EMBO course includes accommodation and transportation to and from the ISG Hotel to the venue

Academia	€550
PhD Student	€550
Industry	€1100

A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

• The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
• If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
• If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants. 

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grants

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.

This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

If you are attending virtually, you can apply for financial assistance in the submission portal by the abstract deadline. Read the instructions on how to apply for financial assistance. Only submissions for financial assistance will be accepted. Presentation abstracts cannot be submitted here and will be declined. 

In your application you will be asked to summarise your current work,  answer questions regarding why your lab cannot fund your attendance, and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.

Selection

The scientific organisers will select the recipients of registration fee waivers during the participant selection process for courses. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.

Further details

Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.

For further information about financial assistance please refer to the FAQ page.

Hotel

Accommodation in twin rooms, including breakfast is included in the registration fee. Accommodation will be booked for the selected participants at the ISG Hotel for the default dates of Sunday 6 April to Monday 14 April (7 nights).

In case you would like to have a single room, please let the hotel know and please note that you will be required to pay an additional cost of 32€ per night (availability of single rooms may be limited). This should be paid by you directly to the ISG Hotel during your stay.

We recommend that all course participants stay at the ISG Hotel for the course.

Travel information

For travel information, please see here.

If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.

For more information in English see here or in German see here.

You can book your ticket here.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For more information about accommodation and travel, please refer to the FAQ page.

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk..

EMBL Merchandise

If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.

Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

• The first aid room is located next to the ATC Registration Desk (room 10- 205).
• Dial the Emergency number 222 from any EMBL internal phone only.
• Where is the accident? What happened? How many injured?
• Emergency number 06221-387 7821 from mobile phones.
• Please report all accidents to the conference officer at the registration desk.

In case of fire …

• Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
• A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
• Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

• Map to walk from ISG Hotel to EMBL

Additional software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

DELETE THIS TAB IF COURSE IS NOT HYBRID!!

Guidelines

• Do not broadcast the course to unregistered participants.
• Do not share your login details with others.
• You are encouraged to tweet and post about the event. Tweet unless the speaker specifically says otherwise, but be mindful of unpublished data. 
• Please do not capture, transmit or redistribute data presented at the meeting.
• Video library of the recorded talks during the event and for 4 weeks afterwards. Please note: at the request of individual speakers, some talks are not recorded.

Additional information can be found in our Code of Conduct.

Health and well-being

It is important to stay healthy and move around, especially when you are attending an event virtually. We have put together a few coffee break stretches and yoga videos in eCampus for you to enjoy during the event.

How to ask questions

Questions during and after the talks can be asked in the live streaming platform. If time runs out or you think of a question later, you can use the EMBL eCampus forum or send a direct message to the speaker/trainer.

Time zone

The programme is planned based on the Europe/Berlin time zone, unless otherwise stated. As many virtual participants are attending from around the world, we do our best to accommodate as many time zones as possible when creating the programme. Please take your time zone into consideration when planning your attendance.

Please find additional information including FAQs and terms and conditions on our Information for Participants page.