Advanced Fluorescence Imaging Techniques

Overview

Course Overview

This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain quantitative information about protein dynamics in living samples.

Audience

This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo.

Modules/Resources

The topics will include fluorescence microscopy techniques and data analysis such as time-lapse microscopy, confocal microscopy, total internal reflection fluorescence microscopy (TIRFM) and super resolution microscopy (PALM, STORM). The course will cover quantitative methods such as fluorescence resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), photoconversion and photoactivation, and will include state-of-the-art data analysis and image processing techniques.

Learning Outcomes

Participants will get an overview about advanced fluorescence imaging techniques.

This course is organised in cooperation with Olympus.

Speakers

Speakers and Trainers

Speakers

Vsevolod V. Belousov

Shemyakin-Ovchinnikov Inst. of Bioorganic Chemistry

Russia

Stefano De Renzis

EMBL Heidelberg,

Germany

Yulia Ermakova,

EMBL Heidelberg,

Germany

Aliaksandr Halavatyi

EMBL Heidelberg,

Germany

Mark Hink

University of Amsterdam,

The Netherlands

Romaine Laine

University College London

United Kingdom

Marko Lampe

EMBL Heidelberg

Germany

Isabel Lastres-Becker

Instituto de Investigaciones “Alberto Sols”

Spain

Pedro Pereira

MRC Laboratory for Molecular Cell Biology at UCL,

UK

Gabriela Plucinska

Utrecht University

The Netherlands

Simone Reber

Humboldt University Berlin

Germany

Sabine Reither

EMBL Heidelberg,

Germany

Christian Tischer

EMBL Heidelberg

Germany

Urs Ziegler

University of Zurich

Switzerland

Scientific Organisers

Florian Eich

Olympus Europa SE & CO.KG

Germany

Rainer Pepperkok

EMBL Heidelberg

Germany

Stefan Terjung

EMBL Heidelberg

Germany

Course Organisers

Nathalie Sneider

EMBL Heidelberg

Germany

Programme