Super-Resolution Microscopy

Overview

Course Overview

This six day advanced course on fluorescence super-resolution microscopy will cover the theory and practical application of current super-resolution microscopy techniques to biological questions. The participants will have sufficient time to use current super-resolution microscopes for Localisation Microscopy (e.g. dSTORM /GSDIM) and STED Microscopy on a variety of fixed and living samples prepared during the course. Invited guest researchers together with experts from the EMBL and Leica Microsystems will ensure that all aspects are covered in depth.

Audience

This course is aimed at researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level) with access to super-resolution microscopy equipment. The participants should have an immediate need to apply these techniques for their biological questions. The course will strongly focus on practical work and is therefore especially suited for people who rely on advanced microscopy for their research.

Modules/Resources

The course will cover the entire range of skills needed to apply super-resolution microscopy in biology. The following topics will be covered in lectures as well as in practical workshops:

• Sample preparation for super-resolution microscopy
• Multi-Colour 3D STED microscopy
• Multi-Colour 3D Localisation Microscopy
• Data analysis for Localisation Microscopy
• Deconvolution of STED images
• Super-Resolution Imaging of living specimen (STED / PAINT)

Learning Outcomes

At the end of the course, the participants will be able to plan an experiment using super-resolution microscopy, acquire high quality data at the microscope and analyse the images with state-of-the-art computational methods.

This course is organised in cooperation with Leica Microsystems.

Speakers

Speakers

Jörg Bewersdorf

Yale University School of Medicine

USA

Francesca Bottanelli

Yale School of Medicine

USA

Susan Cox

King´s College London

UK

Christian Eggeling

University of Oxford

United Kingdom

Jan Ellenberg

EMBL Heidelberg

Germany

Mike Heilemann

Johann Wolfgang Goethe-University Frankfurt

Germany

Marko Lampe

EMBL Heidelberg

Germany

Grazvydas Lukinavicius

Max Planck Institute for Biophysical Chemistry

Germany

Valentin Nägerl

University of Bordeaux

France

Leila Nahidiazar

Oncode Institute

The Netherlands

Jonas Ries

EMBL Heidelberg

Germany

Ulf Schwarz

Leica Microsystems CMS GmbH

Germany

Katrin Willig

MPI of Experimental Medicine

Germany

Trainers

Jana Döhner, University of Zurich, Switzerland

Jens-Peter Gabriel, Leica Microsystems CMS GmbH, Germany

Nathalie Garin, Leica Microsystems CMS GmbH, Germany

Gitta Hamel, Scientific Volume Imaging bv, The Netherlands

Leila Nahidiazar, Oncode Institute, The Netherlands

Oliver Schlicker, Leica Microsystems CMS GmbH, Germany

Falk Schneider, University of Oxford, United Kingdom

Sebastian Schnorrenberg, EMBL Heidelberg, Germany

Ulf Schwarz, Leica Microsystems CMS GmbH, Germany

Erdinc Sezgin, University of Oxford, United Kingdom

Christoph Spahn, Johann Wolfgang Goethe-University Frankfurt, Germany

David Unnersjö Jess, KTH Royal Institute of Technology, Sweden

Hans van der Voort, Scientific Volume Imaging bv, The Netherlands

Nike Walther, EMBL Heidelberg, Germany

Scientific Organisers

Marko Lampe

EMBL Heidelberg

Germany

Rainer Pepperkok

EMBL Heidelberg

Germany

Ulf Schwarz

Leica Microsystems CMS GmbH

Germany

Course Organisers

Tim Nürnberger

EMBL Heidelberg

Germany

Programme