Luis Alvarez
Leica Microsystems
Germany
EMBL Course
EMBL is committed to sharing research advances and sustaining scientific interaction throughout the coronavirus pandemic. We are delighted to announce that the course is going virtual and invite you to join us online.
This six-day comprehensive microscopy course focuses on stimulated emission depletion microscopy (STED) and its application to biological questions. Through lectures, tutorials, demonstrations and practical workshops, the course provides in-depth knowledge on all aspects of STED Nanoscopy. It covers the fundamentals of STED microscopy, different technical implementations and has a strong emphasis on sample preparation – including choice of fluorophores and refractive index matching, as well as image acquisition strategies for fixed samples and living cells. Students will have the opportunity for exercises on state-of the art STED microscopes in which they will acquire high quality nanoscopy data. The faculty consists of Invited guest researchers, experts from the EMBL and Leica Microsystems.
This course is designed for researchers at the early stages of their career in the life sciences (e.g. pre or postdoctoral level), ideally with access to a STED microscope. The participants should have an immediate need to apply the trained skills for their biological questions. The course is strongly centered on practical aspects of STED microscopy and is therefore especially suited for people who rely on STED nanoscopy for their research.
The course will cover the entire range of skills needed to apply STED nanoscopy in biology. The following topics will be covered in lectures, tutorials as well as in practical workshops:
At the end of the course, the participants will be able to design experiments using STED microscopy, prepare high-quality microscopy samples and acquire state-of-the-art nanoscopy images.
This course is organised in cooperation with Leica Microsystems.
“A well-organised course, with a good balance of brilliant workshops, (pre-recorded) talks, tutorials, discussions and “fun parts”. Although this was held in a virtual format, it offered the opportunity to operate/control the system/software remotely. The course helped me to deepen and expand my knowledge in super resolution microscopy (especially STED) and to further optimise and improve my experiments.” – Daniela Bender, Paul-Ehrlich-Institut, Germany
Leica Microsystems
Germany
EMBL Heidelberg
Germany
University of Rouen Normandy
France
Freie University Berlin
Germany
KTH Royal Institute of Technology Stockholm
Sweden
University of Zurich
Switzerland
University of California San Diego
USA
EMBL Heidelberg
Germany
Goethe University Frankfurt
Germany
Max Planck Institute for Medical Research
Germany
EMBL Heidelberg
Germany
EMBL Heidelberg
Germany
University of Tübingen
Germany
Leica Microsystems CMS GmbH
Germany
Leica Microsystems CMS GmbH
Germany
University of Rouen Normandy
France
Freie University Berlin
Germany
University of Cologne
Germany
EMBL Heidelberg
Germany
Leica Microsystems CMS GmbH
Germany
Leica Microsystems CMS GmbH
Germany
Course and Conference Manager
EMBL Heidelberg
Germany
Training Lab Technician
EMBL Heidelberg
Germany
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To find out the equivalent time zone in your location, enter Berlin, the CEST programme time and your city into the Time Zone Converter.
Time (CEST) | Session |
---|---|
12:30-12:45 | Check-in and house notes Marko Lampe – EMBL Heidelberg, Germany |
12:45-13:00 | Welcome and course overview Marko Lampe – EMBL Heidelberg, Germany Ulf Schwarz – Leica Microsystems CMS GmbH, Germany |
13:00-13:30 | Q&A Pre-Course Material & Introduction to Workshops Marko Lampe – EMBL Heidelberg, Germany Ulf Schwarz – Leica Microsystems CMS GmbH, Germany |
13:30-14:30 | Flash Talks Part 1 |
14:30-14:45 | Break |
14:45-15:45 | Flash Talks Part 2 |
15:45 -16:30 | Virtual Social Programme: Bar Mixer |
Time (CEST) | Session |
---|---|
12:30-12:45 | Check-in and overview of the day |
12:45-13:30 | Introduction to STED Microscopy Marko Lampe – EMBL Heidelberg, Germany Ulf Schwarz – Leica Microsystems CMS GmbH, Germany |
13:30-14:15 | Niccolò Banterle – EMBL Heidelberg, Germany |
14:15-14:30 | Break |
14:30-15:15 | Super-resolution imaging of the kidney filter using advanced sample preparation David Unnersjö-Jess – University of Cologne, Germany |
15:15-16:00 | New fluorescent probes for live-cell imaging Kai Johnsson – Max Planck Institute for Medical Research, Germany |
16:00-16:30 | Break |
16:30-18:30 | WS 1 Multi Color STED Imaging of fixed samples –Group 1 Trainers: Marko Lampe, Luis Alvarez, Jana Döhner WS 2 Imaging of Cleared tissue & ExM-STED – Group 2 Trainers: Ulf Schwarz, David Unnersjö-Jess |
Time (CEST) | Session |
---|---|
10:00-12:00 | WS 1 Multi Color STED Imaging of fixed samples – Group 3 Trainers: Marko Lampe, Luis Alvarez, Jana Döhner WS 2 Imaging of Cleared tissue & ExM-STED –Group 4 Trainers: Ulf Schwarz, David Unnersjö-Jess |
12:00-12:30 | Break |
12:30-12:45 | Check-in and overview of the day |
12:45-13:30 | Studying the structure of chromosomes and NPCs in single human cells by super-resolution microscopy Kai Sandvold Beckwith – EMBL Heidelberg, Germany |
13:30-14:15 | Optimization of protocols and image acquisition strategies for live-cell tauSTED Magalie Benard & Damien Schapman – University of Rouen Normandy, France |
14:15- 14:30 | Break |
14:30-15:15 | TauSTED: pushing STED beyond its limits with a lifetime Ulf Schwarz – Leica Microsystems, Germany |
15:15-16:00 | Virtual Social Programme: Speed networking |
16:00-16:30 | Break |
16:30-18:30 | WS 1 Multi Color STED Imaging of fixed samples –Group 1 Trainers: Marko Lampe, Luis Alvarez, Jana Döhner WS 2 Imaging of Cleared tissue & ExM-STED – Group 2 Trainers: Ulf Schwarz, David Unnersjö-Jess |
Time (CEST) | Session |
---|---|
10:00-12:00 | WS 1 Multi Color STED Imaging of fixed samples –Group 4 Trainers: Marko Lampe, Luis Alvarez, Jana Döhner WS 2 Imaging of Cleared tissue & ExM-STED –Group 3 Trainers: Ulf Schwarz, David Unnersjö-Jess |
12:00-12:30 | Break |
12:30-12:45 | Check-in and overview of the day |
12:45 -13:30 | pre-recorded talk Giuseppe Vicidomini – Italian Institute of Technology, Italy |
13:30-14:15 | Investigating cellular logistics with live-cell STED Francesca Bottanelli – Freie Universität Berlin, Germany |
14:15-14:30 | Break |
14:30-15:00 | Tutorial: Sample Preparation 1 – Refractive Index Matching and Optics Ulf Schwarz -Leica Microsystems CMS GmbH, Germany |
15:00-15:15 | EuroBioImaging- Opportunities for Researchers Antje Keppler – Euro-BioImaging Bio-Hub, EMBL Heidelberg, Germany |
15:15-16:00 | Virtual Social Programme: Online pictionary |
16:00-16:30 | Break |
16:30-18:30 | WS 3 Live cell imaging – Group 1 Trainers: Ulf Schwarz, Luis Alvarez WS 4 Advanced STED Imaging – Group 2 Trainers: Marko Lampe, Alexander Stockhammer |
Time (CEST) | Session |
---|---|
10:00-12:00 | WS 3 Live cell imaging – Group 3 Trainers: Ulf Schwarz, Luis Alvarez WS 4 Advanced live cell STED Imaging – Group 4 Trainers: Marko Lampe, Alexander Stockhammer |
12:00-12:30 | Break |
12:30-12:45 | Check-in and overview of the day |
12:45-13:30 | Characterization of organelle dynamics with live-cell nanoscopy Giovanna Coceano – KTH Royal Institute of Technology Stockholm, Sweden |
13:30-14:15 | Tutorial: Sample Preparation 2 – Fluorophores and staining Jana Döhner – University of Zurich, Switzerland Marko Lampe – EMBL Heidelberg, Germany |
14:15-14:30 | Break |
14:30-15:15 | Exchangeable fluorophore labels in super-resolution fluorescence microscopy Mike Heilemann – Goethe University Frankfurt, Germany |
15:15-16:00 | Virtual Social Programme: Bar Mixer |
16:00-16:30 | Break |
16:30-18:30 | WS 3 Live cell imaging – Group 2 Trainers: Ulf Schwarz, Luis Alvarez WS 4 Advanced STED Imaging – Group 1 Trainers: Marko Lampe, Alexander Stockhammer |
Time (CEST) | Session |
---|---|
10:30-12:00 | WS 3 Live cell imaging – Group 4 Trainers: Ulf Schwarz, Luis Alvarez WS 4 Advanced STED Imaging – Group 3 Trainers: Marko Lampe, Alexander Stockhammer |
12:00-12:30 | Break |
12:30-12:45 | Check-in and overview of the day |
12:45-13:30 | Minimal click chemistry-based genetically encoded tags for fluorescent protein labeling in living cells Ivana Nikić-Spiegel – University of Tübingen, Germany |
13:30-14:15 | Tutorial Sample Preparation 3 – Fixation strategy and sample mounting Jana Döhner – University of Zurich, Switzerland Marko Lampe – EMBL Heidelberg, Germany |
14:15-14:30 | Break |
14:30-15:00 | Discussion rounds |
15:00-15:25 | Correlative Light and Electron Microscopy Jana Döhner – University of Zurich, Switzerland |
15:25-15:50 | Live cell labelling – a chemical perspective Michelle Frei – University of California San Diego, USA |
15:50-16:15 | Closing remarks, comments, farewell and group photo |
The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration Fees (include admission and all course materials):
Academia | €125 |
PhD Student | €125 |
Industry | €250 |
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For more detailed information, follow the instructions provided in our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Registration Fee Waivers
All academic and student registrants are invited to apply for a registration fee waiver, provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO. The registration fee waiver covers the registration sum that you have paid to attend the meeting. Conference participants are not required to pre-pay the registration fee to be selected for a fee waiver for a virtual meeting. If you have already paid the registration fee and are awarded a fee waiver, it will be reimbursed after the meeting. Course participants are required to pay the course fee in advance, which will then be reimbursed after the recipient has attended the course.
Applies to selected courses only. Availability will be indicated during the abstract or motivation letter submission process.
This grant covers costs related to your attendance to the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
Applications for financial assistance can be submitted via the submission portal* (for the submission of abstracts for conferences or the submission of motivation letters for courses) by completing the Financial Assistance Application Section (underneath the section for entering abstract/motivation letter information). The link to the portal can be found in the registration confirmation email that you will receive after registering for the conference or course.
For conferences, if you are not submitting an abstract, you can still apply for financial assistance in the submission portal. Read the instructions on how to apply for financial assistance.
Note that priority will be given to those submitting an abstract to present at the conference. In your application you will be asked to answer questions regarding your motivation for applying, and, for registration fee waivers, the reasons why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
*For some events, applications for Childcare Grants will still be done by email. Information about the grant will be sent out shortly after the abstract/motivation letter deadline. Please contact the event Conference Officer if you have any questions.
The scientific organisers will select the recipients of registration fee waivers during the abstract selection process for conferences and the participant selection process for courses. Results will be announced approximately 3 – 4 weeks before the event start date. Selection results do not impact your admission to the meeting. Registration fee waiver selection is based on your current work or study location, your motivation for applying, the reasons for needing financial support and the impact this event will have on your career. Childcare grants are allocated based on career stage, with priority given to early stage researchers.
Check out this list of external funding opportunities or get more information on attending the conference as an event reporter.
For further information about financial assistance please refer to the FAQ page.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Zoom will be used during the course for the live discussion and feedback sessions and the course practicals and networking. More information will be provided closer to the start of the course.
Please find additional information including FAQs and terms and conditions on our Information for Participants page.
Sponsorship Opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
Date: 8 - 15 Jul 2022
Location: Virtual
Deadline(s):
Application: Closed
Organisers:
Contact: Inna Boesselmann