Luiz Alvarez
Leica Microsystems CMS GmbH
Germany
EMBL Course
This course is organised in cooperation with Leica Microsystems.
As fluorescence lifetime-based readouts become more accessible, this course aims to provide a broad understanding of how the lifetime-based information can be leveraged to solve biological questions. This application-centred course will provide insights into how this additional dimension of fluorescence information can help unveil functional and mechanistic information.
This course is aimed at PhD students and Post Docs with experience in fluorescence microscopy. It will enable them to better apply confocal fluorescence microscopy and in particular lifetime-based readouts to their cutting-edge research, disseminate their gained expertise to colleagues at their home institution and, eventually, to develop imaging methods beyond the state-of-the-art.
Please see EMBL’s COVID-19 safety policy for testing and other requirements to attend this on-site course.
Leica Microsystems CMS GmbH
Germany
Université Paris-Saclay
France
Swammerdam Institute for Life Sciences
The Netherlands
Biomedical Center Munich – LMU Munich
Germany
EMBL Heidelberg
Germany
University of Melbourne
Australia
The Netherlands Cancer Institute
The Netherlands
Leica Microsystems CMS GmbH
Germany
Max Planck Institute for Medical Research
Germany
Max Planck Institute for Medical Research
Germany
EMBL Heidelberg
Germany
Leica Microsystems CMS GmbH
Germany
King’s College London
UK
Leica Microsystems CMS GmbH
Germany
Leica Microsystems CMS GmbH
Germany
Leica Microsystems CMS GmbH
Germany
EMBL Heidelberg
Germany
Max Planck Institute for Medical Research
Germany
Course and Conference Team Lead
EMBL Heidelberg
Germany
Training Lab Manager
EMBL Heidelberg
Germany
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Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG hotel to EMBL | ISG hotel bus stop |
09:00 – 09:15 | Welcome & registration | EMBL Imaging Centre |
09:15 – 09:45 | Icebreaking activity | EMBL Imaging Centre |
09:45 – 10:45 | Lecture: Introduction to fluorescence lifetime-based readouts Kees Jalink /Dorus Gadella | EMBL Imaging Centre |
10:45 – 11:00 | Coffee Break | EMBL Imaging Centre |
11:00 – 12:00 | Lecture: Visualization and analysis of lifetime-based information Giulia Ossato – Leica Microsystems CMS GmbH, Germany | EMBL Imaging Centre |
12:00 – 13:00 | Lunch | EMBL Canteen |
13:00 – 14:00 | Lecture: The application of FRET-FLIM to study protein-protein interactions and spatial organization of proteins in the cells, Marie Erard – Université Paris-Saclay, France | EMBL Imaging Centre |
14:00 – 16:00 | Practical sessions 1+2 | EMBL Imaging Centre |
16:00 – 16:15 | Coffee break | EMBL Imaging Centre |
16:15 – 17:45 | Practical sessions 1+2 | EMBL Imaging Centre |
17:45 – 18:00 | Wrap up session | EMBL Imaging Centre |
18:00 – 19:00 | Dinner | EMBL canteen |
19:45 | Bus from EMBL to ISG Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG hotel to EMBL | ISG hotel bus stop |
09:00 – 11:00 | Participants flash talks | EMBL Imaging Centre |
11:00 – 11:15 | Coffee break | EMBL Imaging Centre |
11:15 – 12:15 | Lecture: Time-Domain Fluorescence lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors Kees Jalink – The Netherlands Cancer Institute, The Netherlands | EMBL Imaging Centre |
12:15 – 13:15 | Lunch | EMBL Canteen |
13:15 – 14:00 | Lecture: Fluorescence lifetime-based biosensors Dorus Gadella – Swammerdam Institute for Life Sciences, The Netherlands | EMBL Imaging Centre |
14:00 – 16:00 | Practical sessions 1+2 | EMBL Imaging Centre |
16:00 – 16:15 | Coffee break | EMBL Imaging Centre |
16:15 – 17:45 | Practical sessions 1+2 | EMBL Imaging Centre |
17:45 – 18:00 | Wrap up session | EMBL Imaging Centre |
18:00 – 19:30 | Tips & Tricks with concurrent Pizza dinner | EMBL Imaging Centre |
19:45 | Bus from EMBL to ISG Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG hotel to EMBL | ISG hotel bus stop |
09:00 – 10:00 | Lecture: Introduction to STED microscopy and tauSTED Ulf Schwarz – Leica Microsystems CMS GmbH, Germany | EMBL Imaging Centre |
10:00 – 11:00 | Lecture: STED microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation Mariano Gonzalez Pisfil – Biomedical Center Munich, Germany | EMBL Imaging Centre |
11:00 – 11:15 | Coffee break | EMBL Imaging Centre |
11:15 – 12:00 | Lecture: Label-free imaging and endogenous fluorescence Giulia Ossato – Leica Microsystems CMS GmbH, Germany | EMBL Imaging Centre |
12:00 – 12:15 | Group photo | EMBL Imaging Centre |
12:15 – 13:15 | Lunch | EMBL canteen |
13:15 – 15:45 | Practical sessions 3-6 | EMBL Imaging Centre |
15:45 – 16:15 | Coffee break | EMBL Imaging Centre |
16:15 – 18:45 | Practical sessions 3-6 | EMBL Imaging Centre |
18:45 – 19:00 | Wrap up session | EMBL Imaging Centre |
19:00 – 20:30 | Dinner & discussions | EMBL canteen |
20:35 | Bus from EMBL to ISG Hotel | EMBL bus stop |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG hotel to EMBL | ISG hotel bus stop |
09:00 – 10:00 | Lecture: TauSense Tools making lifetime-based readouts easily accessible Julia Roberti – Leica Microsystems CMS GmbH, Germany | EMBL Imaging Centre |
10:00 – 11:00 | Lecture: Synthetic fluorescent probes for live-cell imaging Kai Jonsson – Max Planck Institute for Medical Research, Germany | EMBL Imaging Centre |
11:00 – 11:15 | Coffee break | EMBL Imaging Centre |
11:15 – 12:15 | Lecture: FP-based Multicolour experiments with FLIM Sergi Padilla-Parra – King’s College, UK | EMBL Imaging Centre |
12:15 – 13:15 | Lunch | EMBL Imaging Centre |
13:15 – 15:45 | Practical sessions 3-6 | EMBL Imaging Centre |
15:45 – 16:15 | Coffee break | EMBL Imaging Centre |
16:15 – 18:45 | Practical sessions 3-6 | EMBL Imaging Centre |
18:45 – 19:15 | Wrap up session | EMBL Imaging Centre |
19:45 | Bus to Heidelberg downtown | EMBL bus stop |
20:00 – 22:00 | Course dinner downtown |
Time | Speaker | Location |
---|---|---|
08:40 | Bus from ISG hotel to EMBL | ISG hotel bus stop |
09:00 – 10:00 | Lecture: The phasor approach to fluorescence lifetime analysis of biosensor Förster resonance energy transfer (FRET) Elisabeth Hinde – University of Melbourne, Australia | EMBL Imaging Centre |
10:00 – 11:00 | Lecture: Open discussion | EMBL Imaging Centre |
11;00 – 11:15 | Coffee break | EMBL Imaging Centre |
11:15 – 12:15 | Practical session: Lifetime based readout analysis and interpretation | EMBL Imaging Centre |
12:15 – 13:15 | Lunch | EMBL Imaging Centre |
13:15 – 15:15 | Practical session: Lifetime based readout analysis and interpretation | EMBL Imaging Centre |
15:15 – 15:30 | Coffee break | EMBL Imaging Centre |
15:30 – 16:15 | Round Table: The future of lifetime-based read outs | |
16:15 – 16:30 | Feedback round and Wrap-up | |
16:30 | Bus to Heidelberg train station and city center | EMBL bus stop |
The course is limited to 20 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €500 |
PhD Student | €500 |
Industry | €1000 |
NO visa support letters will be issued until payment of the registration fee is confirmed.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of both registration fee waivers and travel grants. Availability is limited to participants attending on-site EMBL Courses and virtual or on-site EMBO Practical Courses in Heidelberg, and will be indicated during the motivation letter submission process.
Your place in the course is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
– up to €1000 for participants travelling to an EMBL Course from outside Europe.
– up to €500 for any participant travelling to an EMBO Practical Course.
– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.
– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.
*Registration fees are only covered for EMBO Practical Courses
The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.
Applies to selected courses only. Availability will be indicated during the motivation letter submission process.
This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.
Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):
– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course
– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
Single rooms have been reserved for 6 nights (Sunday 9 July to Saturday 15 July 2023) at the ISG Hotel. After the selection process, selected participants should contact the hotel directly to make a booking (booking code MIC23-03). We recommend that all course participants stay at the ISG Hotel which is the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For further information on getting to EMBL Heidelberg visit our Travel Information page.
For enquiries about accommodation and local transportation please refer to the FAQ page.
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Co-organisers:
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
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Date: 10 - 14 Jul 2023
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Chris Stocks