Heiko Gaethje
Evident Scientific
Germany
EMBL Course
Registration is not yet open for this event. If you are interested in receiving more information please register your interest.
Light microscopy is the most widely used form of microscopy for life science research. The correct handling of the microscope and optimising image quality (e.g. contrast, resolution) as well as experimental design according to different applications are crucial to obtain the best results.
This course will provide the participants with an overview of the basics of microscopy (light and optics, contrast techniques and imaging). The confocal part will include multicolour, 3D, and time-lapse imaging as well as spectral imaging, introduction to photobleaching and photoactivation techniques. Basic image analysis tools including measurement functions, colocalisation, and spectral unmixing as well as 3D/4D image processing will be covered, both in theory and practice. The course will combine lectures with intense daily hands-on practices followed by discussions.
This course is directed towards PhD students and faculty searching for instruction on light microscopy and basic image processing, with basic level on microscopy.
Participants will get an overview of the basics of microscopy.
Please see EMBL’s COVID-19 safety recommendations for testing and other requirements to attend this on-site course.
This course is organised in cooperation with Evident-Olympus.
Evident Scientific
Germany
Evident Scientific
Germany
Evident Scientific
Germany
Evident Scientific
Germany
Evident Scientific
Germany
Evident Scientific
Germany
EMBL Heidelberg
Germany
Course and Conference Officer
EMBL Heidelberg
Germany
Training Laboratory Manager
EMBL Heidelberg
Germany
Are you on social media? Post using #EMBLWidefieldConfocal and don’t forget to tag @EMBLEvents
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | B&B Hotel |
09:00 – 09:15 | Arrival and welcome, house notes | Courtyard Room A & B |
09:15 – 13:00 | Basics of optics and microscopy • Theoretical basics of light and optics • Types of microscope frames – and types of optics • Set up bright field microscopes and the appropriate optics for different needs • Cleaning, centering adjusting and operating a microscope • Basic set-up and use of imaging devices Hands-on session 1 | Courtyard Room A & B |
13:00 – 14:00 | Lunch | EMBL Canteen |
14:00 – 15:00 | Contrast methods • Introduction into contrast methods (phase contrast and differential interference contrast/DIC) | Courtyard Room A & B |
15:00 – 17:30 | Contrast methods – set up and alignment • Set up and use of contrast methods: phase contrast and differential interference contrast/DIC Hands-on session 2 | Courtyard Room A & B |
17:30 – 18:00 | Discussing open questions | Courtyard Room A & B |
18:00 – 19:00 | Dinner | EMBL Canteen |
19:00 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | B&B Hotel |
08:50 – 09:00 | Arrival and coffee | Courtyard Room A & B |
09:00 – 12:30 | Basics of digital imaging • Adaptation of digital cameras and their alignment • Interpretation of image content, histogram and digital filter functions • Improving digital image contrast | Courtyard Room A & B |
12:30 – 13:30 | Lunch | EMBL Canteen |
13:30 – 16:00 | Fluorescence microscopy I • Introduction to fluorescence microscopy • Setting up of microscopes and cameras for fluorescence observation • The different types of illumination sources (e.g. mercury, xenon, metal halide or LED) • Cubes, filters and objectives • Find the appropriate filter combination for a given fluorochrome and application Hands-on session 3 | Courtyard Room A & B |
16:00 – 17:30 | Discussing open questions | Courtyard Room A & B |
17:30 – 18:30 | Pizza dinner | ATC Rooftop Lounge |
18:30 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | B&B Hotel |
08:50 – 09:00 | Arrival and coffee | Courtyard Room A & B |
09:00 – 11:30 | Fluorescence microscopy II • Auto-fluorescence – a source for noise • Digital imaging for fluorescence microscopy • Specific needs of digital cameras • Digital multicolor handling • Acquisition of multidimensional images (3D, time) Hands-on session 3 (continued) | Courtyard Room A & B |
11:30 – 11:35 | Group picture | |
11:35 – 12:30 | Guided tour of the EMBL Advanced light microscopy facility (ALMF) | ALMF |
12:30 – 13:30 | Lunch | EMBL Canteen |
13:30 – 15:00 | Lecture: Introduction to the confocal microscope | Courtyard Room A & B |
15:00 – 18:00 | Hands on session (Group work) Different applications will be covered in small groups. Module 1: Multicolor imaging Module 2: Photomanipulation techniques – FRAP, FLIP and Photoconversion Module 3: 3D image collection and enhancement – zooming, resolution, SNR, z-stacks, IMARIS basic Module 4: Time-lapse imaging, bleaching and viability; Multi-Area Time-Lapses and Stitching | Microscopy rooms |
18:00 – 18:30 | EMBL Bus via ISG Hotel to downtown | ATC Entrance |
18:30 – 20:00 | Free time | |
20:00 – 22:00 | Dinner downtown | Oskar Restaurant u. Vinothek |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | B&B Hotel |
08:50 – 09:00 | Arrival and coffee | Courtyard Room A & B |
09:00 – 10:00 | FRAP and Photoconversion (Talk) | Courtyard Room A & B |
10:00 – 13:00 | Hands-on session (Group work) Work in Modules | Microscopy rooms |
13:00 – 14:00 | Lunch | EMBL Canteen |
14:00 – 18:00 | Hands-on session (Group work) Comparative time-lapse overnight experiment on three different setups | Microscopy rooms |
18:00 – 19:00 | Dinner | EMBL Canteen |
19:00 | Departure to Hotel | ATC Entrance |
Time (Europe/Berlin) | Session | Location |
---|---|---|
08:40 | Bus from hotel to EMBL ATC Entrance | B&B Hotel |
08:50 – 09:00 | Arrival and coffee | Courtyard Room A & B |
09:00 – 09:30 | Evaluation and summary of overnight timelapse acquisition | Courtyard Room A & B |
09:30 – 12:30 | Hands on session (Group work) Work in Modules | Microscopy rooms |
12:30 – 13:00 | Tips and tricks for data presentation | Courtyard Room A & B |
13:00 – 13:10 | Closing remarks | Courtyard Room A & B |
13:10 – 13:25 | Packed lunch | ATC Foyer |
13:25 | EMBL Bus via Hotel to main train station | ATC Entrance |
The course is limited to 12 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.
Registration fees include admission, course materials, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Academia | €525 |
PhD Student | €525 |
Industry | €1050 |
A letter to support your visa application will be issued, on request, once payment of the registration fee is confirmed. We recommend that you book your visa appointment as soon as possible, to avoid any delay with your visa application.
The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.
After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.
Instructions
Please note:
For detailed instructions, please watch our video on how to submit a course motivation letter.
For further information about registration and motivation letter submission please refer to the FAQ page.
Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waivers, travel grants and childcare grants.
Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.
The fee waiver will cover the registration sum that you have paid to attend the course.
The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:
– up to €400 for participants travelling to an EMBL Course within Europe.
There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.
You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.
For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.
The scientific organisers will select the recipients of registration fee waivers and travel grants during the motivation letter or abstract selection process. Results will be announced approximately 6 – 8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the meeting. Selection for registration fee waivers and travel grants is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.
Childcare grants will be allocated in the same timeframe (6-8 weeks before the event start date). Please note that priority will be given to early-stage researchers.
Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.
See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.
For travel information, please see here.
If you are travelling to the conference within Germany then you are eligible for the Deutsche Bahn ‘Event Ticket’ (called the ‘Veranstaltungsticket’ in German). This will result in a lower ticket price if your travel distance to Heidelberg is more than 100 km. You need to provide proof of your event attendance when purchasing the ticket.
For more information in English see here or in German see here.
You can book your ticket here.
Accommodation is not included in the registration fee. Participants are expected to book and pay for their own accommodation. Single rooms have been reserved for 5 nights (21 – 26 April) at B&B Hotel. After the selection process, selected participants should contact the hotel directly (heidelberg@hotelbb.com) to make a booking with the booking code (MIC24-01) by the deadline of Monday, 25 March. We recommend that all course participants stay in the dedicated hotel for the course.
Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
For more information about accommodation and travel, please refer to the FAQ page.
All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.
Please wear your badge at all times when serving yourself.
No food or drinks are allowed in the laboratories.
In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.
If you are interested in purchasing EMBL merchandise (products presented in the glass display in the registration area), please email the EMBL shop to place an order or get in contact with your Course Organiser.
Kindly note the EMBL shop is only open upon request and all purchases must be made in cash (Euros only).
Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.
If first aid is required …
In case of fire …
Beyond first aid…
Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.
Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.
Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.
‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.
There are lockers available on-site to store your luggage, which require a 2 EUR coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.
There is a nursing room available in the ATC Rooftop Lounge on level A29.
During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.
We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.
There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.
A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.
During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.
Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
Below you will find the software and technical requirements for this course.
The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus.
Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.
COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.
Media partners
Imaging and Microscopy, a Wiley magazine
Sponsorship opportunities
We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.
If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.
EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.
Please also note that:
Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.
Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.
Date: 22 - 26 Apr 2024
Location: EMBL Heidelberg
Venue: EMBL Advanced Training Centre
Deadline(s):
Application: Closed
Organisers:
Contact: Catherine Casas-Saavedra