Advanced fluorescence imaging techniques

Overview

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Course overview

This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in mechanisms in cells, tissues and developing organisms. Invited guest researchers together with microscopy experts from EMBL will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain information about localisation and functions of proteins and other bio-molecules at different spatial scales.

Audience

This course is directed towards researchers in the life sciences with access to light microscopy equipment, who have an immediate need to apply the techniques they will learn to solve biological problems at the cellular and molecular level in vivo. As the course will be primarily focused on the advanced techniques, we expect participants to have basic knowledge of light microscopy.

Modules / Resources

The topics will include fluorescence microscopy techniques such as fast time-lapse microscopy of living cells, spinning disk and point-scanning confocal microscopy of large (3D) samples, photo-manipulation as a tool, multicolour single-molecule localization microscopy (SMLM) with spectral demixing, multiplexed imaging, expansion microscopy, FRET-based techniques. For demanding applications, we will cover the aspects of image processing and analysis and the possibilities of microscope automation. The modules will cover all necessary knowledge and demonstrate tools to conduct similar pipelines at your home lab enabling you to answer emerging biological questions.

• This course is organised in cooperation with Evident.

• This course is kindly supported by the EMBL Advanced Light Microscopy Facility.

Learning outcomes

• Get an overview of advanced fluorescence imaging techniques.
• Obtain hands-on experience setting up and running advanced experiments on modern fluorescence microscopes.
• Discuss the possibilities and drawbacks of experiment planning and data interpretation with experts in the field.

What past participants say

“I highly recommend this course if you are looking to take your fluorescence microscopy experiments to the next level.” – Josefina Marin-Rojas, Universidad de Chile, Chile

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

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