Edit
Analysis and integration of transcriptome and proteome data – Course and Conference Office

EMBL Course

Analysis and integration of transcriptome and proteome data

Overview

Course overview

Systems biology is a still expanding field of research aiming to understand at the molecular level how cells, tissues and organisms operate in their biological context. Among the key technologies driving this forward are next-generation sequencing and proteomics, as they provide powerful means to determine global expression levels of genes and proteins. Both fields have matured to a degree that they have now become accessible to researchers in many areas of biology.

During this course, participants will gain theoretical insight in the structure of sequencing and proteomic data, and they will receive training in data analysis for the annotation, quantification, and statistical analysis of gene and protein expression using various computational tools. In addition, training will be provided in the use of Cytoscape to derive biological insights from the integration of both data types.

Audience

This course is targeted at biologists and biochemists who are (starting to be) involved in both next-generation sequencing and mass spectrometry-based proteomics, but who are not experts in these fields.

Modules/resources

  • Principles of mRNA quantification
  • Principles of protein identification and quantification
  • Principles and application of bioinformatic tools for mining of transcriptome and proteome data
  • Data integration by Cytoscape

Learning outcomes

The aim of this practical course is to provide insight in techniques that are frequently used for transcriptome and proteome analysis, and to provide hands-on experience turning primary data into information that can be used for further biological interpretation. To achieve these goals, participants will gain insight in the basic principles of gene expression analysis (RNAseq data quantification and differential expression analysis), protein identification and quantification, and they will be trained in the use of software tools to analyse sequencing and proteomic data (SearchGUI, PeptideShaker, MaxQuant). In addition, the course will provide insight in the complementarity of transcriptome and proteome data by their integrative analysis via Cytoscape.

What past participants say about the course

“An intensive and inspiring course with worldwide top researchers in bioinformatics. Ideal for wet lab scientists who are now starting to work on RNA seq and proteomics analysis” – Marta Furtado, Instituto de Medicina Molecular, Portugal

“In this course, I found lecturers and participants equally willing to share and learn. This, together with the high quality of each lecture and the care of the organisers, contributed to make this week a life-changing experience for me.” – Maria Cristina Petrella, Centre for Genomic Regulation (CRG), Spain

Speakers

Speakers

Scientific organisers

Course organisers

Programme

Are you on social media? Post using #EMBLDataIntegration and don’t forget to tag @EMBLEvents

  • Some pre-course work (watching pre-recordings, protocol reading and computational exercises) with estimated time 0-10 hours will be required and will be shared in advance of the course.
  • All times in the programme below are shown as the time in Europe/Berlin.
  • Some speakers may need to join virtually to give their lecture or Q&A, and the programme is subject to change.
Day 1 – Sunday 4 February 2024
Time
(Europe/Berlin)
SpeakerLocation
08:40 – 09:00Bus from ISG Hotel to EMBL ATC entranceISG Hotel bus stop
09:00 – 09:15 Arrival, welcome and safety introductionsCourtyard Room
09:15- 11:00RNA-seq: cool but is it that simple?
Vladimir Benes – EMBL Heidelberg, Germany
Courtyard Room
11:00 – 11:30Coffee breakCourtyard Foyer
11:30 – 12:30RNAseq: Reads to genes
Raffaele Calogero – University of Torino, Italy
Kristina Roucova – Charles University,
Czech Republic
Courtyard Room
12:30 – 13:30LunchEMBL Canteen
13:30 – 16:00Practical: From Reads to Genes (Single cell RNA-seq)
Raffaele Calogero – University of Torino, Italy
Kristina Roucova – Charles University,
Czech Republic
Computer Training Lab
16:00 – 16:30Coffee breakATC Foyer
16:00 – 18:00Q&A on RNAseq and scRNAseqComputer Training Lab
18:00Departure to ISG HotelATC entrance
18:30DinnerISG Hotel
Day 2 – Monday 5 February 2024
Time
(Europe/Berlin)
SpeakerLocation
08:40 – 09:00Bus from ISG Hotel to EMBL ATC entranceISG Hotel bus stop
09:00 – 10:00Flash talk presentationsComputer Training Lab
10:00 – 12:30Practical & theory: Differential expression analysis
Raffaele Calogero – University of Torino, Italy
Jonathan Landry – EMBL Heidelberg, Germany
Kristina Roucova – Charles University,
Czech Republic
with flexible coffee break
Computer Training Lab
12:30 – 13:30LunchEMBL Canteen
13:30 – 16:00Practical session: Ribosome profiling
Gerben Menschaert – University of Ghent, Belgium
Computer Training Lab
16:00 – 16:30Coffee breakComputer Training Lab
16:30 – 18:30Lecture/practicals: Ribosome profiling
Gerben Menschaert – University of Ghent, Belgium
Computer Training Lab
18:30 – 19:45 DinnerEMBL Canteen
19:45Departure to ISG HotelATC entrance
Day 3 – Tuesday 6 February 2024
Time
(Europe/Berlin)
SpeakerLocation
08:40 – 09:00 Bus from ISG Hotel to EMBL ATC entranceISG Hotel bus stop
09:00 – 11:00Introduction to Proteomics
Jeroen Krijgsveld – German Cancer Research Centre (DKFZ), Germany
Computer Training Lab
11:00 – 11:30Coffee breakFoyer
11:30 – 12:30Lecture: Quantitative proteomics: SILAC and other MS1-based approaches
Jeroen Krijgsveld – German Cancer Research Centre (DKFZ), Germany
Computer Training Lab
12:30 – 13:30LunchEMBL Canteen
13:30 – 15:00Lecture: Quantitative proteomics: TMT
Mikhail Savitski – EMBL Heidelberg, Germany
Computer Training Lab
15:00 – 16:00Lecture: Bioinformatics for proteomics
Lennart Martens – University of Ghent, Belgium
Computer Training Lab
16:00 – 16:30Coffee BreakComputer Training Lab
16:30 – 18:00Q&A to Bioinformatics for proteomics
Lennart Martens – University of Ghent, Belgium
Computer Training Lab
18:00 Shuttle to the restaurantATC entrance
18:30Social dinner downtownEMBL Canteen
Day 4 – Wednesday 7 February 2024
Time
(Europe/Berlin)
SpeakerLocation
08:40 – 09:00Bus from ISG Hotel to EMBL ATC entranceISG Hotel bus stop
09:00 – 10:00Practical Session: Turning data into information-peptide and protein identification, database searching
Lennart Martens – University of Ghent, Belgium
with flexible coffee break
Computer Training Lab
10:00 -10:30Coffee breakATC Foyer
10:30 – 12:00Lecture: Introduction to MaxQuant; analysis of MS data
Daria Fijalkowska – University of Ghent, Belgium
Computer Training Lab
12:00 – 13:00LunchEMBL Canteen
13:00 – 14:30Practical Session: Proteomics data interpretation
Daria Fijalkowska – University of Ghent, Belgium
Computer Training Lab
14:30 – 16:15Lecture: P-values and multiple testing correction
Hyungwon Choi – National University of Singapore, Singapore
Computer Training Lab
16:15Free afternoon
16:15Departure to ISG Hotel and downtownATC entrance
Day 5 – Thursday 8 February 2024
TimeSpeakerLocation
08:40 – 09:00Bus from ISG Hotel to EMBL ATC entranceISG Hotel bus stop
09:00 – 10:00 Q&A to Bioinformatic tools for data comparison and integration
John “Scooter” Morris – University of California, San Francisco
Computer Training Lab
10:00 – 10:30 Coffee break Foyer
10:30 – 12:30Practical: Introduction to Cytoscape
Manjeet Kumar – EMBL Heidelberg, Germany
John “Scooter” Morris – University of California, San Francisco, USA
Santhust – EMBL Heidelberg, Germany
Computer Training Lab
12:30 – 13:30Lunch & group photoEMBL Canteen
13:30 – 15:00Practical: Introduction to Cytoscape
Manjeet Kumar – EMBL Heidelberg, Germany
John “Scooter” Morris – University of California, San Francisco, USA
Computer Training Lab
15:00 – 16:15 Lecture and Practical: Enrichment analysis
Manjeet Kumar – EMBL Heidelberg, Germany
John “Scooter” Morris – University of California, San Francisco, USA
Computer Training Lab
16:15 – 16:45 Coffee BreakFoyer
16:45 – 17:45  Application talk: Network-oriented integrative multi-omics analysis of tissues and fluids – case studies in cancer and cardiovascular diseases
Hyungwon Choi – National University of Singapore, Singapore 
Computer Training Lab
17:45 – 18:45DinnerEMBL Canteen
18:45Shuttle to ISG HotelATC entrance
Day 6 – Friday 9 February 2024
Time SpeakerLocation
08:40 – 09:00  Bus from ISG Hotel to EMBL ATC entranceISG hotel bus stop
09:00 – 12:00 Practical Session: Data integration
John “Scooter” Morris – University of California, San Francisco, USA
with flexible coffee break 
Computer Training Lab
12:00 – 13:00LunchEMBL Canteen
13:00 – 15:00Practical Session: Data integration
John “Scooter” Morris – University of California, San Francisco, USA 
Computer Training Lab
15:00 – 15:30Course wrap-up and farewell Computer Training Lab
15:30Departure to Train Station ATC entrance

Practical information

Registration fees and motivation letter

The course is limited to 24 participants. For selection purposes, please note that your application will not be considered without a letter of motivation.

Registration

Registration fees include admission, course materials, COVID-19 safety measures, meals and coffee breaks.

Participants are expected to book and pay their own accommodation and travel expenses. 

Academia€715
PhD Student€715
Industry€1225

NO visa support letters will be issued until payment of the registration fee is confirmed.

Confirmation and payment

The registration fee should be paid only after acceptance to the course. The results will be announced approximately 2-3 weeks after the application deadline.

Motivation letter submission

After you have logged in and successfully registered, you will receive an email asking you to submit your motivation letter. Click on the link provided and enter your motivation letter in the text box provided. Alternatively you can submit your motivation letter by clicking on the link on the confirmation page directly after registering.

Instructions

  1. Include your relevant skills, experience and qualifications showing why you would be suitable for the course.
  2. Explain why you would like to attend, including what you can contribute and how you think you will benefit from the course.

Please note:

  • The limit of 2000 characters refers to manually typed text and excludes spaces. If an error occurs try using a different web browser (preferably Google Chrome or Mozilla Firefox).
  • If you copy-paste the text into the form, hidden formatting might still be included which may cause the text to exceed the 2,000 character limit resulting in an error message. We recommend you clear all formatting before pasting in the text.
  • If you have special symbols in your text, make sure you are using Unicode characters, otherwise these will not be recognised.

For detailed instructions, please watch our video on how to submit a course motivation letter.

Further details

For further information about registration and motivation letter submission please refer to the FAQ page.

Financial assistance

Financial assistance

Limited financial assistance is provided by the EMBL Advanced Training Centre Corporate Partnership Programme and EMBO in the form of registration fee waiverstravel grants and childcare grants

Your place in the meeting is only confirmed by paying the registration fee, which is mandatory even when receiving a fee waiver.

Registration fee waiver

The fee waiver will cover the registration sum that you have paid to attend the course.

Travel grant

The travel grant will cover the cost of travel (airfare, train, bus, taxi, accommodation, visa, and/or registration fees*) and is provided up to specified caps which are normally as follows:

– up to €400 for participants travelling to an EMBL Course within Europe.

– up to €1000 for participants travelling to an EMBL Course from outside Europe.

– up to €500 for any participant travelling to an EMBO Practical Course.

– up to €1000 for any participant working in Chile, India, Singapore or Taiwan travelling to an EMBO Practical Course.

– up to €700 for any participant working in Croatia, Czech Republic, Estonia, Greece, Hungary, Italy, Lithuania, Luxembourg, Poland, Slovenia, and Turkey travelling to an EMBO Practical Course.

*Registration fees are only covered for EMBO Practical Courses

The organisers may reduce the grant cap to accommodate more participants. Recipients will be notified of their travel cap amount when they are informed of the outcome of their application. Original receipts must be provided with your signature for all costs incurred within two months of completion of travel. Scanned copies cannot be accepted.

Childcare grant

There is the possibility to apply for a childcare grant to offset child care costs incurred by participants, speakers, trainers and organisers when attending a course. Eligible costs include (but are not limited to) fees for a babysitter or child-care facility and travel costs for a caregiver. Please note that priority will be given to early-stage researchers. There is a limited amount of funding available for the childcare grants and funds will be distributed amongst eligible applicants.

Boehringer Ingelheim Fonds travel grant

Applies to selected courses only. Availability will be indicated during the motivation letter submission process.

This grant covers costs related to your attendance at the course (registration, travel and accommodation costs). The grant is restricted to PhD students and postdocs who conduct basic biomedical research.

Whether you are eligible to apply for a travel grant, depends on when you received your university entrance qualification (e.g. Abitur, A-Levels, High School Diploma, Final State Examination):

– for PhD and MD students, as well as graduates, the university entrance qualification must not have been obtained more than 11 years ago at the time of the envisaged course

– for postdocs, the university entrance qualification must not have been obtained more than 13 years ago at the time of the envisaged course

Application

You may apply for financial assistance when submitting your motivation letter for courses. In your application, you will be asked to answer questions regarding why your lab cannot fund your attendance and how your attendance will make a difference to your career. Application for financial support will not affect the outcome of your registration application.

For the Boehringer Ingelheim Fonds Travel Grant, there is a pre-application question during the motivation letter submission process, and if selected you will be requested to complete a standard form and documentation consisting of your travel expense estimation.

Selection

The scientific organisers will select the recipients of all financial assistance during the motivation letter selection process. Results will be announced approximately 6-8 weeks before the event start date, however for some events this may be delayed. Selection results do not impact your admission to the course. Selection is based on scientific merit, your current work or study location, the reasons for needing financial support, and the impact this event will have on your career.

Reimbursement

Costs will be reimbursed after the meeting only once a reimbursement form and original receipts (from travel costs) have been received.

Further details

See our list of external funding opportunities and for further information about financial assistance please refer to the FAQ page.

Accommodation and shuttles

Accommodation is not included in the registration fee. Participants are expected to book and pay for their own accommodation. A mix of single and twin rooms have been reserved for 6 nights (3 – 9 February 2024) at the ISG Hotel.  Single rooms are limited and will be available on a first come, first served basis. After the selection process, selected participants should contact the hotel directly to make a booking with a booking code PRO24-01 by the deadline of 8 January 2024. We recommend that all course participants stay in the dedicated hotel for the course.

Local transport

Shuttle buses will go from the ISG Hotel to EMBL and back, mornings and evenings. A bus schedule and location of the bus stops will be made available prior to the meeting.

Further details

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

For further information on getting to EMBL Heidelberg visit our Travel Information page.

For enquiries about accommodation and local transportation please refer to the FAQ page.

On-site information

Catering

All meals and coffee breaks are included in the registration fee. Our catering staff will prepare a wide variety of vegetarian meals, meat and fish dishes, soups, pasta, fresh fruit and vegetables, as well as a variety of desserts.

Please wear your badge at all times when serving yourself.

No food or drinks are allowed in the laboratories.

Electricity and charging station

In most places the electricity is 220 volts AC (50 cycles). An adaptor and a plug that fits the German socket may be needed for your appliances/laptop (i.e. American, Japanese, etc.). A USB charging station for electronic devices is available at the registration desk.

EMBL Merchandise

If you are interested in purchasing an EMBL souvenir (products presented in the glass display in the registration area), please ask at the registration desk for more information.

Health and safety notes

Please read EMBL’s COVID-19 safety policy for on-site events.
Do not smoke in any EMBL building.
Eating and drinking is prohibited in all laboratories.
Do not enter any restricted areas or the laboratories unless instructed to do so.

If first aid is required …

  • The first aid room is located next to the ATC Registration Desk (room 10- 205).
  • Dial the Emergency number 222 from any EMBL internal phone only.
  • Where is the accident? What happened? How many injured?
  • Emergency number 06221-387 7821 from mobile phones.
  • Please report all accidents to the conference officer at the registration desk.

In case of fire …

  • Press the nearest fire alarm button or the Emergency number 222 from any internal phone.
  • A loud fire alarm will go off if an evacuation is required. On hearing the alarm leave the building immediately following the escape route and fire wardens and head to one of the meeting points
  • Do not use the lifts.

Beyond first aid…

Please remember to bring your own medication, if needed, to the conference. Note that the next pharmacy is a 4-minute drive from the EMBL, but for many medications you will be required to see a doctor to get a prescription.

Ensure in advance that your medical insurance will cover you during your visit in the event that you do need to see a doctor while in Heidelberg. In any case, the EMBL Course and Conference Office will assist you to get to the pharmacy and a doctor of your choice if necessary.

Internet

Wi-Fi is available on campus. Log in using the EMBL-Events network and the event specific password, which will be provided on site. The eduroam network (secure, worldwide roaming access service developed for the international research and education community) is also available.

Lost and found

‘’Lost and Found’’ items are kept at the registration desk until the end of the conference.

Luggage

There are lockers available on-site to store your luggage, which require a 2 EURO coin to operate. There is another luggage room on level E0, which is free to use but remains unlocked during the conference.

Nursing room

There is a nursing room available in the ATC Rooftop Lounge on level A29.

Photography

During the conference, an EMBL Photographer may be taking photos. If you would not like to appear in these, please inform the photographer or a member of the Course and Conference Office.

Printing

We can help to print your boarding pass/train ticket. Please send it to events@embl.de and collect your print-outs at the registration desk.

Room for prayer, yoga, and meditation

There is a room for prayer, meditation, and yoga located on level E0 behind the Auditorium. Please be respectful of others using the room.

Sightseeing

A variety of activities in Heidelberg can be found on the website of Heidelberg Marketing.

Travel to and from the venue

During the event, we provide conference shuttle buses to and from EMBL. In addition, there is the public bus 39A that serves the EMBL campus and taxis can be easily booked at any time. Information on the shuttle buses can be found on the individual event website and more detailed information on travelling to EMBL can be found on our Travel Information page.

Additional information

Address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

Event software

The EMBL eCampus learning platform will be used to collaborate, communicate and network with all of the course participants. All participants will receive information on how to join shortly before the course. We recommend using Chrome, Safari or Mozilla Firefox browsers for eCampus. 

Additional information

Please find additional information including FAQs, terms and conditions, COVID-19 safety policy and travelling to EMBL on our Information for Participants page.

COVID-19 related questions

COVID-19 information for on-site events at EMBL Heidelberg can be found in our COVID-19 FAQs.

Sponsors

Sponsorship opportunities

We offer a variety of event sponsoring possibilities, with the flexibility to select a set sponsorship package or combine individual sponsorship options to suit your event budget. Discounts are available for companies sponsoring multiple events at EMBL Heidelberg. View other events, or contact sponsorship@embl.de for further information.

If you are interested in becoming a media partner of this event, please visit our media partnerships webpage.

Warning

EMBL wishes to warn sponsors of EMBL conferences and courses of fraudulent schemes purporting to offer sponsorship opportunities on behalf of EMBL or affiliated with EMBL officials. One current scam campaign of which we are aware is conducted using the name ‘Judy Eastman’ (judy@gopcontact.a2hosted.com) and entails approaches to sponsors offering sponsorship opportunities on EMBL’s behalf. Please be kindly advised that all relevant communication regarding sponsorship of EMBL conferences, symposia and courses is handled by EMBL directly and is sent from an official EMBL account. EMBL does not work with any external providers on sponsorship acquisition.

Please also note that:

  • EMBL never provides attendee lists for purchase. Any offers of such are fraudulent.
  • EMBL will never call or email you to ask for your credit card details or to request a payment.
  • All payments are on invoice.

Suspicious communications purportedly from, for or on behalf of EMBL should be reported to EMBL at the following email address sponsorship@embl.de.

Media kit

Want to let others know you’re attending this event? Take a look at our shareable media and feel free to use them in your social media channels or presentations.

Social 1080×1920
Social 1080×1080
Social 1600×900
PPT Slide 16×9

Date: 4 - 9 Feb 2024

Location: EMBL Heidelberg

Venue: EMBL Advanced Training Centre


Deadline(s):

Application: Closed


Organisers:

  • Vladimir Benes
    EMBL Heidelberg, Germany
    • Raffaele Calogero
      University of Turin, Italy
      • Daria Fijalkowska
        University of Ghent, Belgium

What's new on our blog

Edit