Rachel Green
Johns Hopkins School of Medicine
USA
EMBL Conference
For late registrations please contact Diah Yulianti at diah.yulianti@embl.de
This conference will take place at EMBL Heidelberg, with the option to attend virtually.
Mechanisms that shape cellular proteomes are critical for life. This conference focuses on the process of protein synthesis, from the molecular and structural understanding of the translational machinery to its regulation, its implications in cell and organismal biology, and its alteration in disease.
The regulation of mRNA translation is critical to maintain cell homeostasis and mediates cellular responses to environmental, physiological, and pathological cues. Given the central importance of protein synthesis, it is unsurprising that dysregulation or defects in mRNA translation are widely linked to disease, including cancer, neurological or metabolic disorders, and viral infection. In recent years, technical advances have challenged hitherto established dogmas, uncovered new quality control mechanisms, expanded the breath of translation regulators, enhanced our understanding of the dynamics of translation in living cells, and revealed unexpected interconnections with other cellular processes such as metabolism. Advances on these aspects of mRNA translation will be featured, as well as new insights into translation regulation in physiology and disease.
This conference is partnered with Cold Spring Harbor Laboratory (CSHL).
Please see EMBL’s COVID-19 safety recommendations if attending the on-site event.
Johns Hopkins School of Medicine
USA
MRC Laboratory of Molecular Biology
UK
University of Cambridge
UK
University of Leeds
UK
University of Illinois at Urbana-Champaign
USA
University of Surrey
UK
SciLifeLab – Karolinska Institutet
Sweden
Hubrecht Institute
The Netherlands
Institute of Biophysics – National Research Council (CNR)
Italy
Additional speakers will be selected from abstracts.
University of Glasgow
UK
Centre for Genomic Regulation (CRG)
Spain
EMBL Heidelberg
Germany
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Time (Europe/Berlin) | Speaker |
---|---|
16:00 – 18:00 | Arrival / Registration with light refreshments Conference Bus schedule (updated 31 Aug) |
16:30 – 17:30 | Pre-conference workshop “Ribosomes are decision hubs that control human embryonic cell fate trajectories“ by Immagina BioTechnology srl (registration required) |
18:00 – 18:10 | Welcome remarks by the scientific organisers |
18:10 – 18:20 | John Hershey Tribute Christopher S. Fraser – University of California, USA |
18:20 – 19:05 | Keynote talk 1 To be introduced by Alfredo Castello – University of Glasgow, UK From stress adaptation to off-target immune response: the physiological consequences of modified mRNA Anne Willis – University of Cambridge, UK |
19:05 – 19:50 | Keynote talk 2 To be introduced by Christian Spahn – Charité Berlin, Germany The colliding ribosome as a hub for translational regulation and signaling activation Rachel Green – Johns Hopkins School of Medicine, USA |
19:50 – 20:15 | Coffee break |
20:15 – 21:00 | Keynote talk 3 To be introduced by Matthias W. Hentze – EMBL Heidelberg, Germany A human translational initiation complex suggests two independent roles for the helicase eIF4A Venki Ramakrishnan – MRC Laboratory of Molecular Biology, UK |
21:00 – 22:30 | Welcome reception with light refreshments |
Time (Europe/Berlin) | Speaker |
---|---|
09:00 – 12:30 | Session 1 – Initiation |
09:00 – 09:10 | Introduction/overview from session chair Julie Aspden – University of Leeds, UK |
09:10 – 09:30 | Head and Embryo 80S monosomes are much more translationally active than 80S in other tissues Julie Aspden – University of Leeds, UK Not available on demand |
09:30 – 09:45 | Fine tuning the Integrated Stress Response through eIF2B regulation with small molecules Alisa Zyryanova – University of Cambridge, UK |
09:45 – 10:00 | Human tumor suppressor protein Pdcd4 binds to the 40S to inhibit translation initiation Jay Brito Querido – University of Michigan Medical School, USA Not available on demand |
10:00 – 10:15 | eIF4F activity is specifically regulated to maintain stem cell self-renewal in the Drosophila testis downstream of JAK/STAT Ruoxu Wang – UCL, UK |
10:15 – 10:30 | Uncovering the rules between the translation of specific mRNAs and their requirements of eIF4A1 activity and function Tobias Schmidt – Beatson Institute for Cancer Research, UK |
10:30 – 11:00 | Coffee break |
11:00 – 11:15 | Translation Initiation in Hypoxia: the case of the oncogene β-catenin Angelita Simonetti – Université de Strasbourg, France |
11:15 – 11:30 | Ribosome queuing induced by a stem-loop in the uORF2/ATF4 overlap represents yet another level of ATF4 translational control Anna Smirnova – Institute of Microbiology of the CAS, Czech Republic |
11:30 – 11:45 | Mechanism of transcript-specific translation repression by eIF3 Fatima Alghoul – Dana-Farber Cancer Institute/ Harvard Medical School, USA |
11:45 – 12:00 | Translating the translation process – developing eIF1A inhibitors as a research tool and potential cancer therapy Daniel Hayat – Weizmann Institute, Israel |
12:00 – 12:15 | Structural pathway of human translation initiation – from 43S PIC assembly to multi-step 48S IC remodeling Niels Fischer – Max Planck Institute for Multidisciplinary Sciences, Germany Not available on demand |
12:15 – 12:30 | Translation initiation at ambiguous start sites: setting the right frame Matthias Erlacher – Medical University of Innsbruck, Austria |
12:30 – 14:00 | Lunch |
14:00 – 17:30 | Session 2 – Ribosome/Elongation |
14:00 – 14:10 | Introduction/overview from session chair Hong Jin – University of Illinois at Urbana-Champaign, USA |
14:10 – 14:30 | From an ancient GTPase promoting protein synthesis in an evolutionarily conserved way to reconsidering translation pauses for control Hong Jin – University of Illinois at Urbana-Champaign, USA |
14:30 – 14:45 | Elucidating novel mechanisms coordinating ribosome biogenesis across cellular compartments Eliana Bianco – ETH Zürich, Switzerland |
14:45 – 15:00 | Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation Markus Höpfler – MRC Laboratory of Molecular Biology, UK |
15:00 – 15:15 | Nascent chain folding and transient polysome rearrangements orchestrate co-translational protein complex assembly in bacteria Jirí Koubek – University of Heidelberg, Germany |
15:15 – 15:30 | Co-translational ribosome pairing enables native assembly of misfolding-prone subunits Sander Tans – AMOLF institute, The Netherlands Not available on demand |
15:30 – 16:00 | Coffee break & Meet the speakers of Day 1 and Day 2 |
16:00 – 16:15 | Identification and characterization of novel quality control factors safeguarding co-translational assembly pathways Ayala Shiber – Technion-Israel Institute of Technology, Israel Not available on demand |
16:15 – 16:30 | rRNA expansion segments: evolutionary tools to enhance translation accuracy Robert Rauscher – University of Bern, Switzerland |
16:30 – 16:45 | The molecular basis of tRNA selectivity by human pseudouridine synthase 3 (PUS3) and its impact on mRNA translation Leon Kleemann – University of Bern, Switzerland |
16:45 – 17:00 | Ribosomal protein paralogues in germline development Katarina Grobicki – University of Cambridge, UK |
17:00 – 17:15 | mRNA selective functions of eIF3 in mRNA translation and cancer Dieter Wolf – Westlake University, China |
17:15 – 17:30 | Translation factor eIF5A is the sensor and effector for autoregulation polyamine synthesis Ivaylo Ivanov – National Institutes of Health, USA |
17:30 – 18:00 | Pre-dinner drinks |
18:00 – 19:30 | Dinner |
19:30 – 21:30 | Poster session 1 (odd numbers) in ATC Helices List of posters |
21:30 – 22:30 | Wine & Cheese in ATC Rooftop Lounge |
Time (Europe/Berlin) | Speaker |
---|---|
09:00 – 11:30 | Session 3 – Methods/Systems approaches |
09:00 – 09:10 | Introduction/overview from session chair Vicent Pelechano – SciLifeLab – Karolinska Institutet, Sweden |
09:10 – 09:30 | Genomic dissection of co‑translational mRNA decay, from metadegradome sequencing to genome‑wide frameshifts Vicent Pelechano – SciLifeLab – Karolinska Institutet, Sweden |
09:30 – 09:45 | In vivo single-cell ribosome profiling to investigate translational regulation in aged epidermal stem cells Clara Duré – University of Zurich, Switzerland |
09:45 – 10:00 | Measuring changes in translation efficiency with single-cell ribosome profiling Michael VanInsberghe – Hubrecht Institute-KNAW, Oncode Institute, and University Medical Center Utrecht, The Netherlands |
10:00 – 10:15 | CITePuro-seq: unlocking the secrets of immune cells plasticity with an innovative translatomic technique Stefania Oliveto – INGM-Unimi, Italy Not available on demand |
10:15 – 10:45 | Coffee break |
10:45 – 11:00 | Comparative CRISPRi screens reveal cell context-dependent quality control during mRNA translation Geraldine Rodschinka – Max Planck Institute of Biochemistry, Germany Not available on demand |
11:00 – 11:15 | NaP-TRAP-seq uncovers changes in the regulatory activity of 5’-UTRs on translation during early vertebrate development Jean-Denis Beaudoin – University of Connecticut Health Center, USA |
11:15 – 11:30 | Modeling tRNA determinants of codon translation efficiency in human cells Xavier Hernandez – Alias Centre for Genomic Regulation, Spain |
11:30 – 16:00 | Free time / Sightseeing |
Session 4 – Interconnections/Turnover | |
16:00 – 16:10 | Introduction/overview from session chair Marvin Tanenbaum – Hubrecht Institute, The Netherlands |
16:10 – 16:30 | Dissecting the translation elongation phase through ultra-long tracking of individual ribosomes Marvin Tanenbaum – Hubrecht Institute, The Netherlands |
16:30 – 16:45 | Old dogmas revisited (and reshaped): A new view of the landscape of bacterial coupled transcription-translation Mikel Irastorza – The Hebrew University of Jerusalem, Israel Not available on demand |
16:45 – 17:00 | Real-time tracking of transcription-translation coupling Olivier Duss – EMBL Heidelberg, Germany Not available on demand |
17:00 – 17:15 | Structural basis of ribosomal 30S subunit degradation by RNase R Helge Paternoga – University of Hamburg, Germany Not available on demand |
17:15 – 17:30 | Co-translational intactions of importins with nascent nuclear cargo Maximilian Seidel – Max Planck Institute of Biophysics, Germany |
17:30 – 18:00 | Coffee break |
18:00 – 18:15 | Variant in EPRS1 mRNA from Hypomyelinating Leukodystrophy Patients Blocks m6A Modification at Variant-distal Sites, and Inhibits Nuclear Export and Translation Paul Fox Lerner – Research Institute, Cleveland Clinic, USA |
18:15 – 18:30 | LARP1 senses free ribosomal subunits to coordinate supply and demand of ribosomal proteins James Saba – Johns Hopkins University School of Medicine, USA |
18:30 – 18:45 | Hypoxia-induced epigenetic changes coordinate transcription start site selection and translational control through remodeling of 5’UTRs Kathleen Watt – Karolinska Institutet, Sweden |
18:45 – 19:00 | mRNA Location and Translation Rate Determine Protein Targeting to Dual Destinations Stavroula Mili – National Cancer Institute, NIH USA |
19:00 – 19:30 | Pre-dinner drinks |
19:30 – 20:30 | Dinner |
20:30 – 22:30 | Poster session 2 (even numbers) in ATC Helices List of posters |
22:30-23:30 | Wine & Cheese in the ATC Foyer |
Time (Europe/Berlin) | Speaker |
---|---|
09:00 – 12:30 | Session 5 – Metabolism/Disease/Viruses |
09:00 – 09:10 | Introduction/overview from session chair Gabriella Viero – Institute of Biophysics – National Research Council (CNR), Italy |
09:10 – 09:30 | Loss of interactions between SMN and ribosomes leads to the disruption of a multifunctional translation platform: implications for Spinal Muscular Atrophy Gabriella Viero – Institute of Biophysics – National Research Council (CNR), Italy |
09:30 – 09:45 | Regulation of poly(A)-tail length and translation in oocytes and early embryos Kehui Xiang – Whitehead Institute for Biomedical Research, USA Not available on demand |
09:45 – 10:00 | 28S rRNA fragment serves as biomarker for early cancer detection Rastislav Horos – Hummingbird Diagnostics GmbH, Germany Not available on demand |
10:00 – 10:15 | Mitochondrial import of the F1-ATP synthase subunit ATP5A1 is enhanced by RNA Aindrila Chatterjee – EMBL Heidelberg, Germany |
10:15 – 10:30 | Regulation of PINK1 translation via modulation of insulin and AMPK signaling to support mitophagy in neurons Jara Tabitha Hees – Max Planck Institute for Biological Intelligence, Germany |
10:30 – 11:00 | Coffee break |
11:00 – 11:15 | Discovery of factorless internal ribosome entry sites through metagenomic data mining Nicolas Salcedo-Porras – The University of British Columbia, Canada |
11:15 – 11:30 | Activation of an antiviral endonuclease, RNase L alters the transcriptional landscape through changes in translation during innate immunity Agnes Karasik – National Institutes of Health / NIDDK, USA |
11:30 – 11:45 | Universal features of Nsp1-mediated translational shutdown by coronaviruses Evangelos Karousis – University of Bern, Switzerland |
11:45 – 12:00 | Human MCTS1-dependent translation of JAK2 is essential for IFN-g immunity to mycobacteria Jonathan Bohlen – Necker Hospital for Sick Children, France |
12:00 – 12:15 | Blocking translation to rescue ALS/FTD phenotypes associated with C9ORF72 repeat expansion Franck Martin – University of Strasbourg, CNRS, France |
12:15 – 12:30 | Role of the RNA-binding protein YBX3 in Mesothelioma Angela Rubio Tenor – MRC-Toxicology Unit, UK |
12:30 – 14:00 | Lunch |
14:00 – 16:00 | Poster session 3 (all numbers) in ATC Helices List of posters |
16:00 – 19:00 | Session 6 – RNP plus RQC |
16:00 – 16:10 | Introduction/overview from session chair André Gerber – University of Surrey, UK |
16:10 – 16:30 | Phosphofructokinase is a conserved RNA-binding protein that modulates cell cycle progression André Gerber – University of Surrey, UK |
16:30 – 16:45 | Large-scale map of RNA binding protein interactomes across the mRNA life-cycle Lena Street – Columbia University, USA |
16:45 – 17:00 | eIF4E1b is a non-canonical eIF4E required for maternal mRNA dormancy Laura Lorenzo Orts – Institute of Molecular Pathology (IMP), Austria |
17:00 – 17:15 | Real-time assembly dynamics of an mRNP translation repression complex Marco Payr – EMBL Heidelberg, Germany |
17:15 – 17:30 | The multifaceted role of FMRP: translational repression, ribosome stalling, and potential implications for mRNA degradation via the no-go decay pathway MaKenzie Scarpitti – The Ohio State University, USA |
17:30 – 17:45 | Ribosome collision underlies ZAKalpha-mediated inflammation and programmed cell death in UV-irradiated skin Simon Bekker-Jensen – University of Copenhagen, Denmark |
17:45 – 18:15 | Coffee Break & Meet the speakers of Day 3 and Day 4 |
18:15 – 18:30 | Sequence context and ribosomal collisions affect the suppressor tRNA efficiency Nikhil Bharti – University of Hamburg, Germany |
18:30 – 18:45 | Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control Petr Tesina, CEITEC MU, Czech Republic Not available on demand |
18:45 – 19:00 | Ubiquitin code for helicase-driven clearance of colliding ribosomes Toshifumi Inada – The University of Tokyo, Japan |
19:00 – 19:15 | The helicase HrpA splits stalled ribosomes in E. coli Annabelle Campbell – Johns Hopkins University, USA |
19:15 – 19:30 | Power naps: a new mechanism of bacterial ribosome hibernation Sergey Melnikov – Newcastle University, UK |
19:30 – 19:45 | Closing remarks |
19:45 – 21:30 | Conference Dinner |
21:30 – 00:30 | Conference Party |
Departure day
For late registrations please contact Diah Yulianti at diah.yulianti@embl.de
On-site registration fees include admission, conference materials, meals and coffee breaks. Participants are expected to book and pay their own accommodation and travel expenses.
Virtual registration fees include access to all of the talks (livestreamed and on demand) and facility to submit questions.
On-site Academia | €775 |
On-site PhD Student | €675 |
On-site Industry | €975 |
Virtual Academia | €200 |
Virtual PhD Student | €150 |
Virtual Industry | €250 |
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Silver sponsor
Media partners
EMBO Journal, an EMBO Press journal
International Union of Biochemistry and Molecular Biology
FEBS Journal, a FEBS Press journal
FEBS Letters, a FEBS Press journal
Bio Essays, a Wiley Online Library
Advanced Biology, a Wiley Online Library
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For on-site participants only.
This industry webinar will be hosted by our sponsor Immagina BioTechnology srl prior to the EMBL Conference: Protein synthesis and translational control
on Wednesday 6 September 2023, 16:30 – 17:30
Participation in this workshop is free of charge for registered on-site conference attendees. The number of available places is limited (first come, first served).
All registered conference attendees will receive an email with a registration link in the beginning of August.
ABSTRACT
Ribosomes are decision hubs that control human embryonic cell fate trajectories
Prof. Leo Kurian
University of Cologne, Germany
The blueprints of developing organs are preset at the early stages of embryogenesis. Transcriptional and epigenetic mechanisms are proposed to preset developmental trajectories. However, we reveal that the competence for the future cardiac fate of human embryonic stem cells (hESCs) is preset in pluripotency by a specialized mRNA translation circuit controlled by RBPMS. RBPMS is recruited to active ribosomes in hESCs to control the translation of essential factors needed for cardiac commitment program, including Wingless/Integrated (WNT) signaling. Consequently, RBPMS loss specifically and severely impedes cardiac mesoderm specification, leading to patterning and morphogenetic defects in human cardiac organoids. Mechanistically, RBPMS specializes mRNA translation, selectively via 3′UTR binding and globally by promoting translation initiation. Accordingly, RBPMS loss causes translation initiation defects highlighted by aberrant retention of the EIF3 complex and depletion of EIF5A from mRNAs, thereby abrogating ribosome recruitment. We demonstrate how future fate trajectories are programmed during embryogenesis by specialized mRNA translation.
Date: 6 - 10 Sep 2023
Location: EMBL Heidelberg and Virtual
Venue: EMBL Advanced Training Centre
Deadline(s):
Abstract submission: Closed
Registration (On-site): Closed
Registration (Virtual): Closed
Organisers:
Contact: Diah Yulianti