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Microinjection in Zebrafish and Medaka: From Transgenesis to CRISPR – Course and Conference Office

EMBL Course

Microinjection in Zebrafish and Medaka: From Transgenesis to CRISPR

Overview
Speakers
Programme

Course Overview

This course will provide participants with hands-on tutorials and the theory behind gene transfer into fish embryo, as well as gene knock-in/-out using CRISPR/Cas. Microinjection into zebrafish and medaka embryos will be covered, as well as subsequent screening to identify positive founder fish.

Audience

This course is aimed at research technicians and scientists interested in the generation of genetic modified fish models. Participants should have basic experience with molecular biology techniques.

Modules/Resources

Microinjection of lineage tracers into one-cell stage embryos
Co-injection of DNA and mRNA into one-cell stage embryos
Microinjection of CRISPR/Cas constructs
Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
Homology directed repair in fish
Screening by imaging
High throughput, high content screening for successful events
Molecular validation of Crispr/Cas9 mediated indel formation

Learning Outcomes

The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.