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EMBL Imaging Centre

Open access to cutting-edge electron and light microscopy

We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology.

Sample Vitrification & Screening

Vitrobot Mark IV

The Vitrobot Mark IV System from Thermo Fisher Scientific is an automated plunge freezer able to vitrify thin samples applied to an electron microscopy grid after removing excess fluid by blotting.


Features

  • Automated vitrification of aqueous suspensions and cells deposited on EM grids
  • Temperature and humidity-controlled environmental chamber

Specifications

  • Working temperature adjustable between +4°C and +60°C
  • Relative humidity adjustable to 100%
Credit: Stuart Ingham/EMBL.
Thermo Fisher Scientific Vitrobot Mark IV. Credit: Stuart Ingham/EMBL.

EM GP2

The EM GP2 from Leica Microsystems is an automated plunge freezer able to vitrify thin samples applied to an electron microscopy grid after removing excess fluid by blotting.


Features

  • Automated vitrification of aqueous suspensions and cells deposited on EM grids
  • Temperature and humidity-controlled environmental chamber
  • Sensor-driven blotting

Specifications

  • Working temperature adjustable between +4°C and +60°C
  • Relative humidity adjustable to 99%
Credit: Stuart Ingham/EMBL.
Leica EM GP2. Credit: Stuart Ingham/EMBL.

Glacios

The Glacios from Thermo Fisher Scientific is a 200 kV transmission electron microscope with a two-condenser lens system. It’s equipped with an autoloader system which allows for quick screening of up to 12 cryo-EM samples. The system also features the latest state-of-the-art SelectrisX energy filter and Falcon 4 electron counting camera which enables high-quality data collection for suitable single particle samples.


Features

  • Autoloader system for easy sample transfer between TFS TEMs
  • State-of-the-art energy filter and electron counting camera

Specifications

  • 200 kV FEG electron source
  • SelectrisX energy filter
  • Falcon 4EC direct electron detector
  • SerialEM software for tailored, automated data acquisition
Cryo-EM micrograph of an exemplary single-particle sample.
Credit: Stuart Ingham/EMBL
Thermo Fisher Scientific Glacios. Credit: Stuart Ingham/EMBL.
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