Studying macromolecular complexes in their native environment within a cell has always been a challenge for scientists. However, with the advancements in technology and new methods, researchers are now able to delve into the ultrastructure of molecules, even in thicker samples, and study them in their natural context. One such method is cryo-Electron Tomography, which is available for users at our facility, which is part of the EMBL Node, through the Euro-BioImaging Proof-of-Concept study. In this interview, Simone Mattei, our Team Leader for Electron Microscopy Service and Technology Development, tell us more about cryo-Electron Tomography and its applications.