STELLARIS 8 STED with TauSTED from Leica Microsystems allows the study of multiple dynamic events simultaneously and investigation of molecular relationships and mechanisms within the cellular context.

FALCON (FAst Lifetime CONtrast) includes phasors for quantitative FLIM analysis. It is a fully integrated solution for fluorescence lifetime imaging (FLIM) and enables video-rate image acquisition for rapid kinetic studies in live cells. 

Features

• Resolving molecular relationships in specimens
• Multiple simultaneous events can be studied at the nanoscale
• 2D and 3D STED imaging
• Significantly reduced light doses due to TauSTED
• Advanced STED-FLIM options with STED and FALCON (full FLIM quantitative tools, phasor FLIM, species separation based on phasor analysis)
• Advanced STED-FCS options
• Fast validation of results on a single platform, from confocal to super-resolution LIGHTNING and STED
• With FALCON it is possible to:
  • follow fast molecular interactions via FLIM-FRET (Förster resonance energy transfer).
  • use biosensors to detect microenvironmental changes, such as pH or ion concentration.
  • apply lifetime contrast to separate multiple fluorophores.
  • do analysis using phasor FLIM provides a 2D visualisation of lifetime components. With FLIM phasors you can follow microenvironmental changes, select components to multiplex signal (phasor separation), and determine FRET efficiency.

Specifications

• Five spectrally tunable Power HyD sensitive photon counting detectors (2 HyD S, 2 HyD X, 1 HyD R)
• Confocal: tunable White Light Lasers (WLL) 440-790 nm, 405 nm STED depletion: 592 nm, 660 nm, 775 nm
• 8kHz Resonant Scanner
• Total system dead-time: 1.5 ns
• TauSTED: Tunable resolution based on lifetime (depending on sample and fluorophore: <30 nm (lateral) and <100 nm (axial). Automatic lifetime-based background suppression algorithm. Light dose reduction (WLL excitation) for all STED lines (592, 660, 775 nm). Available for 2D and 3D STED in live and in fixed specimens, also for multicolor applications. Automated workflow integrated in the LAS X software.
• The STED WHITE glycerol and water objective lenses with motCORR technology provide adaptive optical correction for aberrations introduced by sample inhomogeneities and refractive-index mismatch. The STED WHITE objective lenses provide a working distance of 300 µm:
  • HC PL APO 86x/1.20 W motCORR STED WHITE
  • HC PL APO 93x/1.30 GLYC motCORR STED WHITE
  • HC PL APO 100x/1.40 OIL STED WHITE

STELLARIS STED equipped with TauSTED from Leica Microsystems allows the study of multiple dynamic events simultaneously and investigation of molecular relationships and mechanisms within the cellular context.

Features

• Resolving molecular relationships in specimens
• Multiple simultaneous events can be studied at the nanoscale
• 2D and 3D STED imaging
• Significantly reduced light doses due to TauSTED
• Fast validation of results on a single platform, from confocal to super-resolution LIGHTNING and STED

Specifications

• Five spectrally tunable Power HyD sensitive photon counting detectors (2 HyD S, 2 HyD X, 1 HyD R)
• Confocal: tunable White Light Lasers (WLL) 440-790 nm, 405 nm STED depletion: 592 nm, 660 nm, 775 nm
• 8kHz Resonant Scanner
• Total system dead-time: 1.5 ns
• TauSTED: Tunable resolution based on lifetime (depending on sample and fluorophore: <30 nm (lateral) and <100 nm (axial). Automatic lifetime-based background suppression algorithm. Light dose reduction (WLL excitation) for all STED lines (592, 660, 775 nm). Available for 2D and 3D STED in live and in fixed specimens, also for multicolor applications. Automated workflow integrated in the LAS X software.
• The STED WHITE glycerol and water objective lenses with motCORR technology provide adaptive optical correction for aberrations introduced by sample inhomogeneities and refractive-index mismatch. The STED WHITE objective lenses provide a working distance of 300 µm:
  • HC PL APO 86x/1.20 W motCORR STED WHITE
  • HC PL APO 93x/1.30 GLYC motCORR STED WHITE
  • HC PL APO 100x/1.40 OIL STED WHITE