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Electron Microscopy Core Facility

The facility provides advanced expertise in electron microscopy, from sample preparation to image analysis, for a wide variety of biological samples.

Equipment

Electron Microscopes (EMCF)

These microscopes are maintained and managed by the EM core facility. We provide training and assistance to users who wish to use any of the equipment listed below.

MicroscopeFeaturesRoom
JEM 1400Flash JEOLMicroscope for screening and 2D acquisition. Flexible operation from 80-200kV.316
JEM 2100+
JEOL		Microscope for large-scale 2D screening and tomography. Flexible operation from 80-200kV.317
TECNAI F30 300kV FEG
TFS		tomography microscope (plastic samples), and cryomicroscope, primarily for cellular tomography application322
CrossBeam 550 ZeissFocused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging.311
CrossBeam 540 ZeissFocused Ion Beam-Scanning Electron Microscope (FIB-SEM) for large-volume 3D imaging.320
Gemini 450, Zeiss + 3View, GatanScanning electron microscope combined with a built-in microtome for slice and view 3D imaging of large volumes.IC 15-142

Ultramicrotomes

For plastic sections

Conventional UltramicrotomesRoom
UC7, Leicaplastic-1325
UC7, Leicaplastic-2325

For cryosections

Cryo-ultra-microtomesRoom
UC6, Leicacryo-3, for Tokuyasu cryosections325

Sample preparation

High Pressure Freezer – room 327

Freeze Substitution Devices (FS) ─ room 305A

  • AFS-2a, Leica
    • process samples for manual freeze-substitution and plastic-embedding
  • AFS-2b, Leica
    • process samples for manual freeze-substitution and plastic-embedding
  • AFS-2c, Leica
    • process samples for manual freeze-substitution and plastic-embedding, acquired with the support of the HMLS

Accessory equipment

Leica CPD critical point dryer (room 312)
Bruker Skyscan 1270 MicroCT (room 312)

Leica EM ACE 600 carbon coater (room 312)
Pelco EasiGlow glow discharge machine (room 324)

Computing

Image analysis software

PPMS Online booking

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