Pathogenic proteotoxicity of cryptic splicing is alleviated by ubiquitination and ER-phagy.

Prieto-Garcia C, Matkovic V, Mosler T, Li C, Liang J, Oo JA, Haidle F, Mačinković I, Cabrera-Orefice A, Berkane R, Giuliani G, Xu F, Jacomin AC, Tomaskovic I, Basoglu M, Hoffmann ME, Rathore R, Cetin R, Boutguetait D, Bozkurt S, Hernández Cañás MC, Keller M, Busam J, Shah VJ, Wittig I, Kaulich M, Beli P, Galej WP, Ebersberger I, Wang L, Münch C, Stolz A, Brandes RP, Tse WKF, Eimer S, Stainier DYR, Legewie S, Zarnack K, Müller-McNicoll M, Dikic I

Science (New York, N.Y.), 2024

doi:10.1126/science.adi5295.

Structural basis of the Integrator complex assembly and association with transcription factors.

Razew M, Fraudeau A, Pfleiderer MM, Linares R, Galej WP

Molecular cell, 2024

doi:10.1016/j.molcel.2024.05.009.

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Gemin O, Armijo V, Hons M, Bissardon C, Linares R, Bowler MW, Wolff G, Kovalev K, Babenko A, Salo VT, Schneider S, Rossi C, Lecomte L, Deckers T, Lauzier K, Janocha R, Felisaz F, Sinoir J, Galej W, Mahamid J, Müller CW, Eustermann S, Mattei S, Cipriani F, Papp G

bioRxiv, 2024

doi:10.1101/2024.01.18.576170.

Structure of the human 20S U5 snRNP.

Schneider S, Brandina I, Peter D, Lagad S, Fraudeau A, Portell-Montserrat J, Tholen J, Zhao J, Galej WP

Nature structural & molecular biology, 2024

doi:10.1038/s41594-024-01250-5.

Monovalent metal ion binding promotes the first transesterification reaction in the spliceosome.

Aupič J, Borišek J, Fica SM, Galej WP, Magistrato A

Nature communications, 2023

doi:10.1038/s41467-023-44174-2.

Characterization of the SF3B1-SUGP1 interface reveals how numerous cancer mutations cause mRNA missplicing.

Zhang J, Xie J, Huang J, Liu X, Xu R, Tholen J, Galej WP, Tong L, Manley JL, Liu Z

Genes & development, 2023

doi:10.1101/gad.351154.123.

PIM1 controls GBP1 activity to limit self-damage and to guard against pathogen infection.

Fisch D, Pfleiderer MM, Anastasakou E, Mackie GM, Wendt F, Liu X, Clough B, Lara-Reyna S, Encheva V, Snijders AP, Bando H, Yamamoto M, Beggs AD, Mercer J, Shenoy AR, Wollscheid B, Maslowski KM, Galej WP, Frickel EM

Science (New York, N.Y.), 2023

doi:10.1126/science.adg2253.

A combinatorial approach to uncover an additional Integrator subunit.

Offley SR, Pfleiderer MM, Zucco A, Fraudeau A, Welsh SA, Razew M, Galej WP, Gardini A

Cell reports, 2023

doi:10.1016/j.celrep.2023.112244.

Structural studies of the spliceosome: bridging the gaps.

Tholen J, Galej WP

Current opinion in structural biology, 2022

doi:10.1016/j.sbi.2022.102461.

What's NEXT for the exosome?

Razew M, Galej WP

Molecular cell, 2022

doi:10.1016/j.molcel.2022.06.017.

Emerging insights into the function and structure of the Integrator complex.

Pfleiderer MM, Galej WP

Transcription, 2022

doi:10.1080/21541264.2022.2047583.

Structural basis of branch site recognition by the human spliceosome.

Tholen J, Razew M, Weis F, Galej WP

Science (New York, N.Y.), 2021

doi:10.1126/science.abm4245.

Structural basis for conformational equilibrium of the catalytic spliceosome.

Wilkinson ME, Fica SM, Galej WP, Nagai K

Molecular cell, 2021

doi:10.1016/j.molcel.2021.02.021.

Structure of the catalytic core of the Integrator complex.

Pfleiderer MM, Galej WP

Molecular cell, 2021

doi:10.1016/j.molcel.2021.01.005.

Inhibition of bacterial ubiquitin ligases by SidJ-calmodulin-catalysed glutamylation.

Bhogaraju S, Bonn F, Mukherjee R, Adams M, Pfleiderer MM, Galej WP, Matkovic V, Lopez-Mosqueda J, Kalayil S, Shin D, Dikic I

Nature, 2019

doi:10.1038/s41586-019-1440-8.

Structural studies of the spliceosome: past, present and future perspectives.

Galej WP

Biochemical Society transactions, 2018

doi:10.1042/BST20170240.

Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures.

Galej WP, Toor N, Newman AJ, Nagai K

Chemical reviews, 2018

doi:10.1021/acs.chemrev.7b00499.

Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.

Wilkinson ME, Fica SM, Galej WP, Norman CM, Newman AJ, Nagai K

Science (New York, N.Y.), 2017

doi:10.1126/science.aar3729.

Structure of a spliceosome remodelled for exon ligation.

Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K

Nature, 2017

doi:10.1038/nature21078.

Cryo-EM structure of the spliceosome immediately after branching.

Galej WP, Wilkinson ME, Fica SM, Oubridge C, Newman AJ, Nagai K

Nature, 2016

doi:10.1038/nature19316.

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 A resolution.

Nguyen TH, Galej WP, Bai XC, Oubridge C, Newman AJ, Scheres SH, Nagai K

Nature, 2016

doi:10.1038/nature16940.

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.

Nguyen TH, Galej WP, Fica SM, Lin PC, Newman AJ, Nagai K

Current opinion in structural biology, 2016

doi:10.1016/j.sbi.2015.12.005.

The architecture of the spliceosomal U4/U6.U5 tri-snRNP.

Nguyen TH, Galej WP, Bai XC, Savva CG, Newman AJ, Scheres SH, Nagai K

Nature, 2015

doi:10.1038/nature14548.

Structural studies of the spliceosome: zooming into the heart of the machine.

Galej WP, Nguyen TH, Newman AJ, Nagai K

Current opinion in structural biology, 2014

doi:10.1016/j.sbi.2013.12.002.

Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.

Nguyen TH, Li J, Galej WP, Oshikane H, Newman AJ, Nagai K

Structure (London, England : 1993), 2013

doi:10.1016/j.str.2013.04.017.

Crystal structure of Prp8 reveals active site cavity of the spliceosome.

Galej WP, Oubridge C, Newman AJ, Nagai K

Nature, 2013

doi:10.1038/nature11843.