Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.

Defelipe LA, Veith K, Burastero O, Kupriianova T, Bento I, Skruzny M, Kölbel K, Uetrecht C, Thuenauer R, García-Alai MM

Nature communications, 2024

doi:10.1038/s41467-024-54037-z.

Corrigendum to "SARS-CoV-2 Mpro oligomerization as a potential target for therapy" [Int. J. Biol. Macromol. 267 (Part 1) (May 2024) 131392].

Lis K, Plewka J, Menezes F, Bielecka E, Chykunova Y, Pustelny K, Niebling S, Garcia AS, Garcia-Alai M, Popowicz GM, Nowak JS, Czarna A, Kantyka T, Pyrc K

International journal of biological macromolecules, 2024

doi:10.1016/j.ijbiomac.2024.133473.

Raynals, an online tool for the analysis of dynamic light scattering.

Burastero O, Draper-Barr G, Raynal B, Chevreuil M, England P, Garcia Alai M

Acta crystallographica. Section D, Structural biology, 2023

doi:10.1107/s2059798323004862.

Biophysical characterization of membrane proteins.

Niebling S, Burastero O, García-Alai M

Methods in molecular biology (Clifton, N.J.), 2023

doi:10.1007/978-1-0716-3147-8_12.

Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans.

Hassani Nia F, Woike D, Bento I, Niebling S, Tibbe D, Schulz K, Hirnet D, Skiba M, Hönck HH, Veith K, Günther C, Scholz T, Bierhals T, Driemeyer J, Bend R, Failla AV, Lohr C, Alai MG, Kreienkamp HJ

Molecular psychiatry, 2022

doi:10.1038/s41380-022-01882-3.

New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain.

Sander S, Müller I, Alai MG, Nicke A, Tidow H

The Journal of biological chemistry, 2022

doi:10.1016/j.jbc.2022.102495.

Antiviral activity of natural phenolic compounds in complex at an allosteric site of SARS-CoV-2 papain-like protease.

Srinivasan V, Brognaro H, Prabhu PR, de Souza EE, Günther S, Reinke PYA, Lane TJ, Ginn H, Han H, Ewert W, Sprenger J, Koua FHM, Falke S, Werner N, Andaleeb H, Ullah N, Franca BA, Wang M, Barra ALC, Perbandt M, Schwinzer M, Schmidt C, Brings L, Lorenzen K, Schubert R, Machado RRG, Candido ED, Oliveira DBL, Durigon EL, Niebling S, Garcia AS, Yefanov O, Lieske J, Gelisio L, Domaracky M, Middendorf P, Groessler M, Trost F, Galchenkova M, Mashhour AR, Saouane S, Hakanpää J, Wolf M, Alai MG, Turk D, Pearson AR, Chapman HN, Hinrichs W, Wrenger C, Meents A, Betzel C

Communications biology, 2022

doi:10.1038/s42003-022-03737-7.

The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils.

Bücker R, Seuring C, Cazey C, Veith K, García-Alai M, Grünewald K, Landau M

Nature communications, 2022

doi:10.1038/s41467-022-32039-z.

Cosolvent sites-based discovery of Mycobacterium tuberculosis protein kinase G inhibitors.

Burastero O, Defelipe LA, Gola G, Tateosian NL, Lopez ED, Martinena CB, Arcon JP, Traian MD, Wetzler DE, Bento I, Barril X, Ramirez J, Marti MA, Garcia-Alai MM, Turjanski AG

Journal of medicinal chemistry, 2022

doi:10.1021/acs.jmedchem.1c02012.

Biophysical screening pipeline for cryo-EM grid preparation of membrane proteins.

Niebling S, Veith K, Vollmer B, Lizarrondo J, Burastero O, Schiller J, Struve García A, Lewe P, Seuring C, Witt S, García-Alai M

Frontiers in molecular biosciences, 2022

doi:10.3389/fmolb.2022.882288.

Opening opportunities for Kd determination and screening of MHC peptide complexes.

Kopicki JD, Saikia A, Niebling S, Günther C, Anjanappa R, Garcia-Alai M, Springer S, Uetrecht C

Communications biology, 2022

doi:10.1038/s42003-022-03366-0.

Hydrazones and thiosemicarbazones targeting protein-protein-Interactions of SARS-CoV-2 papain-like protease.

Ewert W, Günther S, Miglioli F, Falke S, Reinke PYA, Niebling S, Günther C, Han H, Srinivasan V, Brognaro H, Lieske J, Lorenzen K, Garcia-Alai MM, Betzel C, Carcelli M, Hinrichs W, Rogolino D, Meents A

Frontiers in chemistry, 2022

doi:10.3389/fchem.2022.832431.

Dissociation of β2m from MHC class I Triggers formation of Noncovalent, transient heavy chain dimers.

Dirscherl C, Löchte S, Hein Z, Kopicki JD, Harders AR, Linden N, Karner A, Preiner J, Weghuber J, Garcia-Alai M, Uetrecht C, Zacharias M, Piehler J, Lanzerstorfer P, Springer S

Journal of cell science, 2022

doi:10.1242/jcs.259498.

Cyclohexyl-α maltoside as a highly efficient tool for membrane protein studies.

Missel JW, Salustros N, Becares ER, Steffen JH, Laursen AG, Garcia AS, Garcia-Alai MM, Kolar Č, Gourdon P, Gotfryd K

Current research in structural biology, 2021

doi:10.1016/j.crstbi.2021.03.002.

Correction to: reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.

López-Méndez B, Baron B, Brautigam CA, Jowitt TA, Knauer SH, Uebel S, Williams MA, Sedivy A, Abian O, Abreu C, Adamczyk M, Bal W, Berger S, Buell AK, Carolis C, Daviter T, Fish A, Garcia-Alai M, Guenther C, Hamacek J, Holková J, Houser J, Johnson C, Kelly S, Leech A, Mas C, Matulis D, McLaughlin SH, Montserret R, Nasreddine R, Nehmé R, Nguyen Q, Ortega-Alarcón D, Perez K, Pirc K, Piszczek G, Podobnik M, Rodrigo N, Rokov-Plavec J, Schaefer S, Sharpe T, Southall J, Staunton D, Tavares P, Vanek O, Weyand M, Wu D

European biophysics journal : EBJ, 2021

doi:10.1007/s00249-021-01573-x.

eSPC: an online data-analysis platform for molecular biophysics.

Burastero O, Niebling S, Defelipe LA, Günther C, Struve A, Garcia Alai MM

Acta crystallographica. Section D, Structural biology, 2021

doi:10.1107/S2059798321008998.

Deamidation drives molecular aging of the SARS-CoV-2 spike protein receptor-binding motif.

Lorenzo R, Defelipe LA, Aliperti L, Niebling S, Custódio TF, Löw C, Schwarz JJ, Remans K, Craig PO, Otero LH, Klinke S, García-Alai M, Sánchez IE, Alonso LG

The Journal of biological chemistry, 2021

doi:10.1016/j.jbc.2021.101175.

Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.

Lizarrondo J, Klebl DP, Niebling S, Abella M, Schroer MA, Mertens HDT, Veith K, Thuenauer R, Svergun DI, Skruzny M, Sobott F, Muench SP, Garcia-Alai MM

Nature communications, 2021

doi:10.1038/s41467-021-23151-7.

Quality control of protein reagents for the improvement of research data reproducibility.

de Marco A, Berrow N, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Uebel S, Raynal B

Nature communications, 2021

doi:10.1038/s41467-021-23167-z.

FoldAffinity: binding affinities from nDSF experiments.

Niebling S, Burastero O, Bürgi J, Günther C, Defelipe LA, Sander S, Gattkowski E, Anjanappa R, Wilmanns M, Springer S, Tidow H, García-Alai M

Scientific reports, 2021

doi:10.1038/s41598-021-88985-z.

Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale survey.

Berrow N, de Marco A, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Uebel S, Raynal B

European biophysics journal : EBJ, 2021

doi:10.1007/s00249-021-01528-2.

Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study.

López-Méndez B, Baron B, Brautigam CA, Jowitt TA, Knauer SH, Uebel S, Williams MA, Sedivy A, Abian O, Abreu C, Adamczyk M, Bal W, Berger S, Buell AK, Carolis C, Daviter T, Fish A, Garcia-Alai M, Guenther C, Hamacek J, Holková J, Houser J, Johnson C, Kelly S, Leech A, Mas C, Matulis D, McLaughlin SH, Montserret R, Nasreddine R, Nehmé R, Nguyen Q, Ortega-Alarcón D, Perez K, Pirc K, Piszczek G, Podobnik M, Rodrigo N, Rokov-Plavec J, Schaefer S, Sharpe T, Southall J, Staunton D, Tavares P, Vanek O, Weyand M, Wu D

European biophysics journal : EBJ, 2021

doi:10.1007/s00249-021-01532-6.

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease.

Günther S, Reinke PYA, Fernández-García Y, Lieske J, Lane TJ, Ginn HM, Koua FHM, Ehrt C, Ewert W, Oberthuer D, Yefanov O, Meier S, Lorenzen K, Krichel B, Kopicki JD, Gelisio L, Brehm W, Dunkel I, Seychell B, Gieseler H, Norton-Baker B, Escudero-Pérez B, Domaracky M, Saouane S, Tolstikova A, White TA, Hänle A, Groessler M, Fleckenstein H, Trost F, Galchenkova M, Gevorkov Y, Li C, Awel S, Peck A, Barthelmess M, Schluenzen F, Lourdu Xavier P, Werner N, Andaleeb H, Ullah N, Falke S, Srinivasan V, França BA, Schwinzer M, Brognaro H, Rogers C, Melo D, Zaitseva-Doyle JJ, Knoska J, Peña-Murillo GE, Mashhour AR, Hennicke V, Fischer P, Hakanpää J, Meyer J, Gribbon P, Ellinger B, Kuzikov M, Wolf M, Beccari AR, Bourenkov G, von Stetten D, Pompidor G, Bento I, Panneerselvam S, Karpics I, Schneider TR, Garcia-Alai MM, Niebling S, Günther C, Schmidt C, Schubert R, Han H, Boger J, Monteiro DCF, Zhang L, Sun X, Pletzer-Zelgert J, Wollenhaupt J, Feiler CG, Weiss MS, Schulz EC, Mehrabi P, Karničar K, Usenik A, Loboda J, Tidow H, Chari A, Hilgenfeld R, Uetrecht C, Cox R, Zaliani A, Beck T, Rarey M, Günther S, Turk D, Hinrichs W, Chapman HN, Pearson AR, Betzel C, Meents A

Science (New York, N.Y.), 2021

doi:10.1126/science.abf7945.

In-depth interrogation of protein thermal unfolding data with MoltenProt.

Kotov V, Mlynek G, Vesper O, Pletzer M, Wald J, Teixeira-Duarte CM, Celia H, Garcia-Alai M, Nussberger S, Buchanan SK, Morais-Cabral JH, Loew C, Djinovic-Carugo K, Marlovits TC

Protein science : a publication of the Protein Society, 2020

doi:10.1002/pro.3986.

Structures of peptide-free and partially loaded MHC class I molecules reveal mechanisms of peptide selection.

Anjanappa R, Garcia-Alai M, Kopicki JD, Lockhauserbäumer J, Aboelmagd M, Hinrichs J, Nemtanu IM, Uetrecht C, Zacharias M, Springer S, Meijers R

Nature communications, 2020

doi:10.1038/s41467-020-14862-4.

Further insights from structural mass spectrometry into endocytosis adaptor protein assemblies.

Heidemann J, Kolbel K, Konijnenberg A, Van Dyck J, Garcia-Alai M, Meijers R, Sobott F, Uetrecht C

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2020

doi:10.1016/j.ijms.2019.116240.

Quality control of purified proteins to improve research data reproducibility.

Uebel S, de Marco A, Berrow N, Lebendiker M, Garcia-Alai M, Knauer SH, Lopez-Mendez B, Matagne A, Parret A, Remans K, Raynal B

Journal of biomolecular techniques : JBT, 2019

doi:.

High-throughput peptide–MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules.

Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D

Science immunology, 2019

doi:10.1126/sciimmunol.aav0860.

Empty peptide-receptive MHC class I molecules for efficient detection of antigen-specific T cells.

Saini SK, Tamhane T, Anjanappa R, Saikia A, Ramskov S, Donia M, Svane IM, Jakobsen SN, Garcia-Alai M, Zacharias M, Meijers R, Springer S, Hadrup SR

Science immunology, 2019

doi:10.1126/sciimmunol.aau9039.

High-throughput stability screening for detergent-solubilized membrane proteins.

Kotov V, Bartels K, Veith K, Josts I, Subhramanyam UKT, Günther C, Labahn J, Marlovits TC, Moraes I, Tidow H, Löw C, Garcia-Alai MM

Scientific reports, 2019

doi:10.1038/s41598-019-46686-8.

Novel CaM-binding motif in its NudT9H domain contributes to temperature sensitivity of TRPM2.

Gattkowski E, Johnsen A, Bauche A, Möckl F, Kulow F, Garcia Alai M, Rutherford TJ, Fliegert R, Tidow H

Biochimica et biophysica acta. Molecular cell research, 2018

doi:10.1016/j.bbamcr.2018.12.010.

Epsin and Sla2 form assemblies through phospholipid interfaces.

Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R

Nature communications, 2018

doi:10.1038/s41467-017-02443-x.

Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit.

Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H

Acta crystallographica. Section F, Structural biology communications, 2017

doi:10.1107/S2053230X17017381.

Identification of the first small-molecule ligand of the neuronal receptor sortilin and structure determination of the receptor-ligand complex.

Andersen JL, Schrøder TJ, Christensen S, Strandbygård D, Pallesen LT, García-Alai MM, Lindberg S, Langgård M, Eskildsen JC, David L, Tagmose L, Simonsen KB, Maltas PJ, Rønn LC, de Jong IE, Malik IJ, Egebjerg J, Karlsson JJ, Uppalanchi S, Sakumudi DR, Eradi P, Watson SP, Thirup S

Acta crystallographica. Section D, Biological crystallography, 2014

doi:10.1107/S1399004713030149.

Genetically directing ɛ-N, N-dimethyl-L-lysine in recombinant histones.

Nguyen DP, Garcia Alai MM, Virdee S, Chin JW

Chemistry & biology, 2010

doi:10.1016/j.chembiol.2010.07.013.

The structure of the FYR domain of transforming growth factor beta regulator 1.

García-Alai MM, Allen MD, Joerger AC, Bycroft M

Protein science : a publication of the Protein Society, 2010

doi:10.1002/pro.404.

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome.

Neumann H, Wang K, Davis L, Garcia-Alai M, Chin JW

Nature, 2010

doi:10.1038/nature08817.

The human papillomavirus E7-E2 interaction mechanism in vitro reveals a finely tuned system for modulating available E7 and E2 proteins.

Smal C, Wetzler DE, Dantur KI, Chemes LB, Garcia-Alai MM, Dellarole M, Alonso LG, Gaston K, de Prat-Gay G

Biochemistry, 2009

doi:10.1021/bi901415k.

Genetically encoding N(epsilon)-methyl-L-lysine in recombinant histones.

Nguyen DP, Garcia Alai MM, Kapadnis PB, Neumann H, Chin JW

Journal of the American Chemical Society, 2009

doi:10.1021/ja906603s.

The novel p53 isoform "delta p53" is a misfolded protein and does not bind the p21 promoter site.

García-Alai MM, Tidow H, Natan E, Townsley FM, Veprintsev DB, Fersht AR

Protein science : a publication of the Protein Society, 2008

doi:10.1110/ps.036996.108.

The N-terminal module of HPV16 E7 is an intrinsically disordered domain that confers conformational and recognition plasticity to the oncoprotein.

García-Alai MM, Alonso LG, de Prat-Gay G

Biochemistry, 2007

doi:10.1021/bi7007917.

High-risk HPV E6 oncoproteins assemble into large oligomers that allow localization of endogenous species in prototypic HPV-transformed cell lines.

García-Alai MM, Dantur KI, Smal C, Pietrasanta L, de Prat-Gay G

Biochemistry, 2007

doi:10.1021/bi602457q.

Molecular basis for phosphorylation-dependent, PEST-mediated protein turnover.

García-Alai MM, Gallo M, Salame M, Wetzler DE, McBride AA, Paci M, Cicero DO, de Prat-Gay G

Structure (London, England : 1993), 2006

doi:10.1016/j.str.2005.11.012.

Chaperone holdase activity of human papillomavirus E7 oncoprotein.

Alonso LG, Smal C, Garcia-Alai MM, Chemes L, Salame M, de Prat-Gay G

Biochemistry, 2006

doi:10.1021/bi0522549.

Casein Kinase II phosphorylation-induced conformational switch triggers degradation of the papillomavirus E2 protein.

Penrose KJ, Garcia-Alai M, de Prat-Gay G, McBride AA

The Journal of biological chemistry, 2004

doi:10.1074/jbc.M314340200.

The HPV16 E7 viral oncoprotein self-assembles into defined spherical oligomers.

Alonso LG, García-Alai MM, Smal C, Centeno JM, Iacono R, Castaño E, Gualfetti P, de Prat-Gay G

Biochemistry, 2004

doi:10.1021/bi036037o.

High-risk (HPV16) human papillomavirus E7 oncoprotein is highly stable and extended, with conformational transitions that could explain its multiple cellular binding partners.

Alonso LG, García-Alai MM, Nadra AD, Lapeña AN, Almeida FL, Gualfetti P, Prat-Gay GD

Biochemistry, 2002

doi:10.1021/bi025579n.

Andes virus and first case report of Bermejo virus causing fatal pulmonary syndrome.

Padula P, Della Valle MG, Alai MG, Cortada P, Villagra M, Gianella A

Emerging infectious diseases, 2002

doi:10.3201/eid0804.010300.