Our unit operates on a complete-service fully costed model, tailoring our charges based on researcher affiliation.
For advice on using CRISPR/Cas9 in your research or should you require a novel transgenic mouse or cell line, please email: gevf@embl.it
For advice on using viruses for your research or ordering a virus, please email: viruses@embl.it
Mouse and cell service elements include:
- CRISPR/Cas9 project design and reagent sourcing
- Zygote pronuclear microinjection
- Surgical embryo implantation
- Microinjection of targeted ES cells into host 8-cell embryos
- Sequence confirmation of F1 genotype
- Cryopreservation of embryos and/or sperm
IVF rederivation
- Import and rederivation of mouse lines into the LAR facility
Virus service elements include:
Production of custom-made purified and quantitated viral stocks that are ready for cargo delivery to the desired cell type.
Recombinant Adeno-Associated Virus (rAAV)
- Vector capacity: 4.7Kb
- Cargo remains episomal
- Current serotypes in stock include rAAV1, 2, 1/2 chimeric, 2-retro, DJ, DJ/8, DJ/9, 5, 6, 7, 8, 8BP2, 9, PHP.S, PHP.EB, B10, and 7m8.
- Please let us know if you require another serotype and we will do what we can to obtain the necessary reagents.
- Typically provide at least 200μl of ≥1×1012 VG/ml but smaller or larger quantities can be provided at request.
Lentivirus
- Vector capacity: 8Kb
- Cargo integrates
- Currently, we utilise VSV-G due to its wide range of infectivity. Other envelope proteins could be offered if requested. Currently, viruses are produced using either the 2nd or 3rd generation.
Herpes Simplex Virus (HSV)
- Vector capacity: 30Kb
- Cargo remains episomal
DNA constructs
- Long ssDNA (up to ~5kb) for CRISPR/Cas9-mediated knock-in
- Modification of DNA, precise to the base pair
- Targeting construct to make a knock-in or knockout
- General sub-cloning service