Hands-on Course

In situ sequencing (ISS) is a spatial transcriptomic method to analyse gene expression in large tissue sections at single cell resolution (1, 2, 3). Using combinatorial barcoding, ISS can identify up to ~300 genes simultaneously, being an ideal tool for the discovery of new cell types, understanding cell-cell heterogeneity, generation of single cell atlases, and more.

Contents

• An overview of spatial transcriptomics
• An introduction to ISS 
• A step-by-step protocol 
• Insights into probe design 
• Information on the ideal number of imaging cycles 
• Hands-on experience on ISS applications (case study)

Final program 2024